稳定性重氮色盐对蚕丝的低温偶合染色性能

为了研究高耐湿处理色牢度蚕丝低温染色方法,文章制备了两种色基对应的四氟硼酸重氮色盐并成功分离,通过1H-NMR、FT-IR验证了所合成重氮色盐分子的化学结构。通过测定不同存储方式下重氮液及色盐溶液的紫外—可见光吸收光谱来计算吸光度的变化率,定量分析并证明了所合成的色盐具有较好的存储稳定性。通过对比不同染色工艺和染色对象的K/S值,揭示了重氮色盐单独使用或在色酚存在下对蚕丝的偶合显色机理,证明了稳定性四氟硼酸重氮色盐可用于蚕丝室温条件下染色,所染蚕丝织物的耐皂洗色牢度和耐摩擦色牢度均在4级以上,可为开发高品质蚕丝低温染色技术提供一条新的途径。

反相高效液相色谱法测定色盐杆菌新种ST307的DNA G+C mol%含量

目的:使用反相高效液相色谱法测定色盐杆菌新种ST307的DNAG+Cmol%含量。方法:以Escherichia coli DH5α为标准菌株,采用90%重蒸水10%甲醇为流动相,检测波长260nm,流速1ml.min-1,在VenusilM P C18柱上对四种碱基进行分离。结果:DNA碱基分离效果好,以外标法计算得到标准菌株DH5α的DNAG+Cmol%含量为50.3%,待测菌株ST307的DNAG+Cmol%含量为60.5%。结论:采用反向高效液相色谱法测定色盐杆菌的DNAG+Cmol%含量准确可靠。

色盐蓝VB眼烧伤的探讨

总结了30年来治疗色盐蓝VB眼烧伤的经验,观察了本病的症状、体征及治疗效果。并做了兔眼实验性的病理观察,通过色盐蓝VB的化学反应探讨了其致病机理,指出色盐蓝VB眼烧伤的预后是良好的伤眼,无永久性视力损害。

李春平《盐色》中“盐道”文学空间的建构

《盐色》是陕西作家李春平“盐道三部曲”的第三部,它在《盐道》《盐味》已构建的文学地理空间之下继续对地理真实与文学真实的动态和谐展开书写,作者在对自身地理经验、地理情感的合理抒发中,用更丰富的史料研究、更充实的现地研究、更明确的文学本体意识、政治立场,续写了在真实地理空间中构建生动文学地理空间的又一范本,扩宽了文学地理学理论研究空间,为研究文学家地理经验与其文学创作的内在关联提供了样本,也为同类文学创作的发展给出了可资借鉴的实例。

“单纯”与“静穆”:李春平《盐色》及其系列小说的美学特征

李春平新作《盐色》与之前的《盐道》《盐味》在审美取向上是统一的。在整体叙事中形成了“单纯”“静穆”的美学特质。围绕陕南特定地域和历史文化特征,营造小说叙事空间和时间;以中国民间社会纯正精神和价值观为小说人物塑造的审美底色。小说文体上的“散文化”“诗化”追求,小说中稳定的叙事空间,静邃的自然书写,形成了此系列小说特有的趋静、审美静观的“静穆”美学特征。

山柰挥发油化学成分的气相色谱/质谱法分析

采用水蒸气蒸馏法提取山柰中的挥发油,经过气相色谱/质谱(GC/MS)联用法分析,鉴定了挥发油中的28个化学成分,其中主要成分有对甲氧基肉桂酸乙酯(59.96%)、肉桂酸乙酯(23.68%)、δ-3-蒈烯(6.61%)等,占挥发油总量的90.25%。

极端嗜盐菌筛选鉴定及诱导碳酸盐矿化研究

以胶南尹家山盐场采集的盐泥样品为基础,用标准方法筛选得到7株到耐25%盐度的菌,选取生长较好的嗜盐菌LMZ2作为研究对象,分别对菌株进行了形态学、生理生化和分子生物学鉴定,并测定了生长曲线,pH曲线及碳酸酐酶曲线,设置不同镁钙比(分别为0、3、5、7、9),以嗜盐菌LMZ2为工作菌株,在25%盐度,pH 7.2条件下进行了较系统的碳酸盐微生物诱导矿化实验。结果表明,嗜盐菌LMZ2 (Genbank登录号:MH430039)为革兰氏阴性菌,不产芽孢,产氨气,产接触酶,经综合鉴定LMZ2为一株以色列色盐杆菌(Chromohalobacter israelensis)。LMZ2在150小时后OD值可达到0.8,在225小时pH上升至8.0左右,不产碳酸酐酶。镁钙比为0时只有方解石,镁钙比在3、5、7可以诱导出组合为单水方解石、富镁方解石和碳酸钙镁石等含镁碳酸盐矿物,镁钙比9时为单水方解石。当有镁离子存在的情况下,嗜盐菌诱导的矿物晶粒和结晶度更好。该研究表明极端嗜盐菌在高盐环境中和高镁因素叠加有力促进了含镁碳酸盐矿物和亚稳定碳酸盐矿物的生成,为解释地质记录中相关碳酸盐矿物的形成机理提供了重要的参考。

克服滚筒印花的传色

滚筒印花法中的传色是影响产、质量的重要技术关键,它往往是确定工艺的重要因素之一。防止传色目前所采用的措施主要有:使用小刀、消色剂和光板滚筒。本文拟着重从小刀和消色剂的使用,结合实际作肤浅的讨论。 一、防止传色的方法及其比较 1.小刀 小刀是防止传色最有效的措施,劳动强度比较轻,操作比较简便,使用上基本不受限制,既不影响对花,又不增加机器负荷,

四氢嘧啶羟化酶的克隆表达、酶学性质及其在羟基四氢嘧啶合成中的研究

四氢嘧啶羟化酶(EctD)是双加氧酶超家族的重要成员,其底物四氢嘧啶和产物羟基四氢嘧啶的应用广泛,因此EctD在生物制造领域具有重要的应用价值。从来源于新疆盐湖的需盐色盐杆菌(Chromohalobacter salexigens)中获得四氢嘧啶羟化酶基因(ectD),并在E.coli BL21(DE3)中进行表达,对异源表达的EctD酶学性质进行研究。结果表明:EctD的最适温度是30℃、最适pH是7.5,在30℃、pH 6.5~8.0条件下具有较好的稳定性;Fe^(2+)、Ba^(2+)、Mn^(2+)、Mg^(2+)、Fe^(3+)和Ca^(2+)离子可增强EctD的酶活,而CO_(2)+、Zn^(2+)和Cu^(2+)离子明显抑制EctD的酶活;动力学参数为K_(m)=7.63 mmol/L、k_(cat)/K_(m)=1.011 L/(mmol·s)、V_(max)=6.75μmol/(mL·min)。在最适条件下,该酶以70 mmol/L四氢嘧啶为底物,催化反应25 h后,可生成68 mmol/L的羟基四氢嘧啶,底物转化率可达98%。本研究通过对EctD的表达和表征,为酶法合成羟基四氢嘧啶奠定基础。

骨和软骨的组织化学方法——2磷酸酶

显示酸性及碱性磷酸酶的方法很多。但是经典的金属盐沉淀法由于受到多方面因素的影响可以出现假阴性、假阳性以及错误的定位(1),较理想的还是用偶氮法(2,3)。然而偶氮法由于底质及重氮盐的不同,反应结果也不一致。萘酚AS磷酸酯是一种取代萘酚(substituted naphthols)。

Gel Filtration Chromatography Combined with Bradford Method for Determination of Total Residual Protein in Ferment Antibiotics

Aim A novel method has been developed for evaluation of the levels of total residual protein in antibiotics produced by fermentation using gel filtration chromatography (GFC) combined with Bradford assay based on determination of residual protein in lincomycin hydrochloride. Methods The chromatographic conditions were SuperdexTM peptide column, 0.01 mol*L-1 phosphate buffer solution as mobile phase, and flow rate of 1 mL·min-1. Five hundred microliters of lincomycin hydrochloride solution (3 g of lincomycin hydrochloride dissolved in 10 mL of mobile phase) was injected into the chromatograph and the eluted solution was collected between 6 min and 14.5 min (protein eluted from column within this period), and the residual content of total protein in the eluted solution was assayed using Bradford assay method. Results The average recovery was more than 90% for bovine serum albumin, the calibration equation for the range of 0-12 μg·mL-1 of protein was y=-0.002 4x2+0.064 2x+0.002 9, r2=0.999 9, RSD=0.1%-0.9%, and the LOD and LOQ were 3 and 10 ng·mL-1 of protein, respectively. Conclusion The novel method for determining the residual protein in ferment antibio-tics is simple, rapid, and precise.

Effects of Salt Stress on Root Vitality of Limonium bicolor (Bunge) Kuntze

[Objective] This study aimed to investigate the effects of different salt concentrations on the root vitality of Limonium bicolor （Bunge） Kuntze. [Method] Limonium bicolor （Bunge） Kuntze was treated with 0, 100, 200 and 400 mmol/L NaCl, respectively. After two weeks, root vitality, biomass and other physiological indicators were measured. [Result] Salt stress had significant influences on the growth of roots. Root vitality of Limonium bicolor increased firstly and reduced gradually with the increase of salt concentration. [Conclusion] The results indicate that Limonium bicolor has certain salt tolerance ability at low salt concentrations; under relatively high salt concentrations, Limonium bicolor roots can maintain high vitality; however, with the continuous increase of salt concentration, Limonium bicolor roots are damaged, with decreasing vitality.

HTK液与含血心脏停搏液对复杂冠状动脉病变合并左心功能不全心肌的保护作用

目的比较组氨酸—色氨酸—酮戊二酸盐液(HTK液)与含血心脏停搏液对复杂冠状动脉病变合并左心功能不全心肌的保护作用。方法采用随机分组的方法将134例复杂冠状动脉病变合并左心功能不全的患者分为两组,每组67例,分别使用HTK液和含血心脏停搏液,比较两组手术前后血清心肌损伤标志物水平、钾、钠离子水平、心功能以及不良事件发生率。结果两组患者治疗前后以及主动脉开放12h、24h、72h肌钙蛋白、肌酸激酶同工酶、钾离子、钠离子、左心室射血分数、左心室收缩末期容积指数、左心室舒张末期容积指数等指标的差异具有统计学意义(P<0.05);两组患者主动脉阻断时间、主动脉开放到心脏复跳、体外循环时间、多巴胺用量比较差异有统计学意义(P<0.01);两组患者围术期不良事件发生情况差异有统计学意义(P<0.01)。结论 HTK液与含血心脏停搏液均可对复杂冠状动脉病变合并左心功能的不全的患者提供安全有效的心肌保护效果,但是HTK液的效果优于含血心脏停搏液,不良事件的发生率更低,安全性更高。

Chromosomes and Karyotypes of Three Seagrass Species of Hydrocharitaceae

[Objective] This study aimed to investigate the chromosomes and karyotypes of three seagrass species of hydrocharitaceae-Enhalus acoroides, Thalassia hemprichii and Halophila minor collected from Li’an Lagoon, Hainan Province, China. [Method] The root-tips of E. acoroides, T. hemprichii and the axillary buds of H. minor were selected as the materials in this study. The conventional crushing method was used to prepare the slice specimens of chromosomes, and the karyotypes of the three species were analyzed. [Result] The chromosome numbers of E. acoroides, T. hemprichii and H. minor were 2n=18, 18, 28, respectively. The karyotype formulas were K （2n）=18= 12m＋6sm, K（2n）=18=12m＋6sm and K（2n）=28=16m＋8sm＋4st, respectively. According to the standard of Stebbins, the karyotypes of E. acoroides and T. hemprichii were 2A, and that of H. minor was 2B. There was no B chromosome and satellite in these three species. [Conclusion] According to the comparison on the karyotypes of this three species, E. acoroides and T. hemprichii are similar in karyotypes to each other, indicating that there is close, inter-relationship between E. acoroides and T. hemprichii.

《黑龙江外纪》所反映的清代黑龙江社会经济

一、《黑龙江外纪》的主要内容 《黑龙江外纪》为清人西清所撰。该书凡八卷,卷一述山川形势沿革;卷二述城堡台站卡伦;卷三述部落、种族、户口、官制、兵制;卷四述俸饷、钱粮、出入款项;卷五述贡品、风俗、刑律、互市;卷六述谪戍、方言、服食及红白事件;卷七述历任职官及流徙谪籍人物;卷八达五谷、果蔬、物产。 作者西清系鄂尔泰之后裔。

QTLs Mapping for Salinity Tolerance at Seedling Stage or Rice(Oryza sativa L.) Using Chromosome Segment Substitution Lines

In this study, a population of chromosome segment substitution lines （CSSLs） derived from the cross between 9311 （indica） and Nipponbare （japonica） was employed to map the quantitative trait loci （QTLs） for salt tolerance under the salt stress simulated with 0.5% NaCI, using survival rate as the index. The data were analyzed by QTL IciMapping v3.1, and the results showed that one QTL （QSsr3） related to salt tolerance was located in the vicinity of the marker RM1350 on chromosome 3, into a genetic interval of 113.2-132.8 cM, with a contribution rate of 17.75%. The additive effect was 10.9, indicating that the QTL derived from the parent Nipponbare improved the salt tolerance of rice at seedling stage. This study will provide a theoretical basis for the selection of salt tolerant rice germplasm.

Determination of penehyclidine hydrochloride in beagle dog plasma by liquid chromatography-electrospray ionization mass spectrometry and the pharmacokinetic study

To develop a fast and sensitive liquid chromatography-mass spectrometry method for the determination of penehyclidine hydrochloride （PH） in beagle dog plasma. PH and diphenhydramine hydrochloride （internal standard, IS） were extracted with a solvent mixture of petroleum ether-ethyl ether （7：3）. Chromatographic separation was achieved on a reversed-phase Eclipse XDB-C18 column （4.6 mm × 150 mm, 5 um） using the eluent of methanol-water （5 mmol/L ammonium acetate） （90：10, v/v, pH 5.8） as mobile phase. The electrospray ionization source was set at the positive multiple reaction monitoring （MRM） mode. This method involved the use of the [M＋H]^＋ ions of PH and diphenhydramine hydrochloride at m/z 316.4- 128.2 and m/z 256.4-167.2. The calibration curve was linear in the range of 1-1000 ng/mL with a correlation coefficient of 0.9988. The lower limit of quantification was 0.05 ng/mL. The precision, accuracy and recovery of the method were acceptable. Following intravenous injection admires＇ tration at doses of 0.5, 1 and 5 mg/kg PH, the main pharmacokinetic parameters were as the followings, t1/2a 0.33 h, t1/2β 2.44 1% tmax 0.058 1% AUC and Cmax exhibited a linear increase along with the increase of dose. The two-compartment model fit the three dose groups. This method was sensitive, accurate and fast for the determination of concentration of PH in beagle dog plasma. It could be used in pharmacokinetic studies of PH.

Determination of Dehydroepiandrosterone Sulfate with High Performance Liquid Chromatography

本文报道了用高效液相色谱-示差检测法(HPLC-RI)测定去氢表雄酮硫酸酯钠盐(DHA-S)及其制剂含量的方法。采用反相 ODS C_(18)色谱柱,乙腈-水(含0.8%磷酸和10μmol/L 四丁基氢氧化铵)作流动相,研究了色谱条件、回收率、检测限量及线性范围。

Application of Tertiary Amine Cationic Polyacrylamide with High Cationic Degree in Salt-free Dyeing of Reactive Dyes

Tertiary amine cationic polyacrylamide with high cationization degree was used as a new cationic agent to pretreat cotton with dip-pad-bake method. The obtained cationic cotton was dyed with reactive dyes in the ab-sence of electrolyte. The effects of the characteristics of the cationic agent and the pretreatment conditions on dye-ability of reactive dye were investigated. The results showed that the fixation and K/S values of the reactive dyes on the cationic cotton were improved compared with those on the untreated one in the presence of salt. Tests on fast-ness properties of the dyed cotton and fabric quality of the pretreated cotton were carried out and the results showed that wash and rub fastness of the salt-free dyeing were both satisfactory. And anti-crease property,tensile and tear strength,and handling of the cationic cotton were also good compared with that of the untreated one.

Synthesis and physico-chemical properties of new green electrolyte 1-butyl-3-methylimidazolium perchlorate

1-butyl-3-methylimidazolium perchlorate([BMIM]ClO4) was synthesized by two steps with N-methylimidazolium.Some physico-chemical properties,such as density,surface tension,viscosity,electrical conductivity as well as electrochemical window,were investigated and solvent performance was also studied.The results show that this kind of ionic liquid is an excellent electrolyte with low viscosity,high electrical conductivity and wide electrochemical window.In addition,[BMIM]ClO4 is soluble in most conventional solvents and some metal oxides have high solubility in it,which lays the foundation of direct electrolysis of metal oxides in this ionic liquid.