Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase...Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase(Mn P)from cassava residue by Phanerochaete chrysosporium in solid state fermentation, the stimulators of Mn P production were screened and their concentrations were optimized by one-at-a-time experiment and Box–Behnken design. The maximum Mn P activity of 186.38 nkat·g-1dry mass of the sample was achieved after 6 days of fermentation with the supplement of 79.5 mmol·L-1·kg-1acetic acid, 3.21 ml·kg-1soybean oil, and 28.5 g·kg-1alkaline lignin, indicating that cassava residue is a promising substrate for Mn P production in solid state fermentation. Meanwhile, in vitro decolorization of indigo carmine by the crude Mn P was also carried out, attaining the ratio of 90.18% after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by Mn P was proposed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandem mass spectrometry. Using the crude Mn P produced from cassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents.展开更多
The volatile chemical components of Radix Paeoniae Rubra (RPR) were analyzed by gas chromatography-mass spectrometry with the method of heuristic evolving latent projections and overall volume integration. The results...The volatile chemical components of Radix Paeoniae Rubra (RPR) were analyzed by gas chromatography-mass spectrometry with the method of heuristic evolving latent projections and overall volume integration. The results show that 38 volatile chemical components of RPR are determined, accounting for 95.21% of total contents of volatile chemical components of RPR. The main volatile chemical components of RPR are (Z, Z)-9,12-octadecadienoic acid, n-hexadecanoic acid, 2-hydroxy- benzaldehyde, 1-(2-hydroxy-4-methoxyphenyl)-ethanone, 6,6-dimethyl-bicyclo[3.1.1] heptane-2-methanol, 4,7-dimethyl-benzofuran, 4-(1-methylethenyl)-1-cyclohexene-1-carboxaldehyde, and cyclohexadecane.展开更多
Conversion of green house CO2 into fuel gas in the presence of reducing agent sources that are Fe and Zn powder, as well as hydrogen ions supplier such as nitric and acetic acids by photo catalytic reduction with wate...Conversion of green house CO2 into fuel gas in the presence of reducing agent sources that are Fe and Zn powder, as well as hydrogen ions supplier such as nitric and acetic acids by photo catalytic reduction with water vapor on TiO2 has been studied. The photo reduction was carried out by batch technique, using a UV lamp with a wave length of 290-400 nm, as a photon source. The gasses produced from the photo reduction were determined by gas chromatography. The research results indicate that the photo reduction of CO2 has successfully produced methane as a dominant product. The presence of the metals in addition to be able to improve the methane yield, it is also able to induce ethylene formation. Meanwhile the acids can considerably enhance the methane yield without formation of ethylene. Furthermore, the enhancement is observed to be controlled by mass of the metals, and the acid concentrations. It is also found that Zn and HNO3 show stronger effect in the increasing CO2 photo reduction.展开更多
基金Supported by the Science&Technology Program of Jiangsu Province(BE2011623)the Scientific Research Project of Provincial Environmental Protection Bureau of Jiangsu Province(2012047)
文摘Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase(Mn P)from cassava residue by Phanerochaete chrysosporium in solid state fermentation, the stimulators of Mn P production were screened and their concentrations were optimized by one-at-a-time experiment and Box–Behnken design. The maximum Mn P activity of 186.38 nkat·g-1dry mass of the sample was achieved after 6 days of fermentation with the supplement of 79.5 mmol·L-1·kg-1acetic acid, 3.21 ml·kg-1soybean oil, and 28.5 g·kg-1alkaline lignin, indicating that cassava residue is a promising substrate for Mn P production in solid state fermentation. Meanwhile, in vitro decolorization of indigo carmine by the crude Mn P was also carried out, attaining the ratio of 90.18% after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by Mn P was proposed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandem mass spectrometry. Using the crude Mn P produced from cassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents.
基金Project(20235020) supported by the National Natural Science Foundation of China
文摘The volatile chemical components of Radix Paeoniae Rubra (RPR) were analyzed by gas chromatography-mass spectrometry with the method of heuristic evolving latent projections and overall volume integration. The results show that 38 volatile chemical components of RPR are determined, accounting for 95.21% of total contents of volatile chemical components of RPR. The main volatile chemical components of RPR are (Z, Z)-9,12-octadecadienoic acid, n-hexadecanoic acid, 2-hydroxy- benzaldehyde, 1-(2-hydroxy-4-methoxyphenyl)-ethanone, 6,6-dimethyl-bicyclo[3.1.1] heptane-2-methanol, 4,7-dimethyl-benzofuran, 4-(1-methylethenyl)-1-cyclohexene-1-carboxaldehyde, and cyclohexadecane.
文摘Conversion of green house CO2 into fuel gas in the presence of reducing agent sources that are Fe and Zn powder, as well as hydrogen ions supplier such as nitric and acetic acids by photo catalytic reduction with water vapor on TiO2 has been studied. The photo reduction was carried out by batch technique, using a UV lamp with a wave length of 290-400 nm, as a photon source. The gasses produced from the photo reduction were determined by gas chromatography. The research results indicate that the photo reduction of CO2 has successfully produced methane as a dominant product. The presence of the metals in addition to be able to improve the methane yield, it is also able to induce ethylene formation. Meanwhile the acids can considerably enhance the methane yield without formation of ethylene. Furthermore, the enhancement is observed to be controlled by mass of the metals, and the acid concentrations. It is also found that Zn and HNO3 show stronger effect in the increasing CO2 photo reduction.
