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基于数字图像技术的薄层色谱定量分析方法研究
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作者 张岳 《广西轻工业》 2010年第6期30-31,共2页
尝试用普通扫描仪进行薄层色谱法定量分析,用普通扫描仪将显色后的硅胶薄层板的图像记录下来,再通过计算机对色谱的数字图像进行处理,挖掘数字图像中所含信息,计算斑点中样品的含量,来实现对样品的定量分析的目的。
关键词 图像技术 薄层色谱定量分析 回收率
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气相色谱分析学习指导
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作者 邱红心 《高等函授学报(自然科学版)》 1997年第4期51-53,共3页
关键词 气相分析 学习指导 仪器分析 固定相 色谱定量分析
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偏苯三甲酸三(异)辛酯中2-乙基己醇的色谱分析 被引量:1
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作者 柴祖明 周玉成 《精细石油化工》 CAS CSCD 1990年第1期59-60,共2页
阐述了选用癸醇作内标物对偏苯三甲酸三(异)辛酪中2-乙基己醇进行定量的色谱分析方法.本法快速、准碗、灵敏、适用于生产控制分析. 一、前言偏苯三甲酸三(异)辛酯(简称三辛酯,缩写为TOTM),是一种性能优良的高温增塑剂。以偏苯三甲酸酐... 阐述了选用癸醇作内标物对偏苯三甲酸三(异)辛酪中2-乙基己醇进行定量的色谱分析方法.本法快速、准碗、灵敏、适用于生产控制分析. 一、前言偏苯三甲酸三(异)辛酯(简称三辛酯,缩写为TOTM),是一种性能优良的高温增塑剂。以偏苯三甲酸酐和2-乙基己醇合成TOTM时。 展开更多
关键词 乙基己醇 偏苯三甲酸 邻苯二甲酸 生产控制 内标物 色谱定量分析 内标法 汽化温度 定量 气相
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1%农乐霉素悬浮剂的高效液相色谱分析 被引量:4
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作者 赵莉 陈雪松 《农药》 CAS 北大核心 2005年第2期72-73,共2页
研究用高效液相色谱法分析1%农乐霉素中有效成分的含量,采用梯度流动相,检测波长为250nm。该方法在50 ̄1000mg/L的进样范围内和峰面积呈线性,相关系数为0.9997。回收率一般在98.5% ̄101.3%之间,变异系数为1.66%。
关键词 农乐霉素 定量分析 高效液相
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多维气相色谱法检测酒中甲醇研究
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作者 刘星辰 《食品界》 2017年第9期74-74,共1页
实验目的(1)掌握色谱定量分析的原理和方法(外标法);(2)了解气相色谱仪氢火焰离子检测器FID的性能和操作方法;(3)了解气相色谱法在产品质量控制中的应用;(4)学习气相色谱法测定甲醇含量的分析方法。实验原理甲醇、乙醇:一般... 实验目的(1)掌握色谱定量分析的原理和方法(外标法);(2)了解气相色谱仪氢火焰离子检测器FID的性能和操作方法;(3)了解气相色谱法在产品质量控制中的应用;(4)学习气相色谱法测定甲醇含量的分析方法。实验原理甲醇、乙醇:一般白酒酿制过程中不可避免地会有甲醇产生。甲醇是一种无色透明的液体,其性质具有高度的挥发性能,对人体的危害很大。甲醇可以被人体吸收, 展开更多
关键词 多维气相 气相 外标法 离子检测器 操作方法 氢火焰 体吸收 色谱定量分析 操作条件 组分含量
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兽药残留检测方法验证探究
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作者 任展 夏梦雯 《南方农业》 2021年第18期187-189,共3页
实验室引入新标准方法开展兽药残留检测时,应在实验室内对标准方法进行验证。因此,根据农业部2386号公告、NY/T 1896-2010、GB/T 27417-2017、GB/T 35655-2017介绍了如何对兽药残留色谱定量分析方法进行验证,以期为县级农产品质量安全... 实验室引入新标准方法开展兽药残留检测时,应在实验室内对标准方法进行验证。因此,根据农业部2386号公告、NY/T 1896-2010、GB/T 27417-2017、GB/T 35655-2017介绍了如何对兽药残留色谱定量分析方法进行验证,以期为县级农产品质量安全检测实验室开展兽药残留检测工作提供参考。 展开更多
关键词 兽药残留检测 标准方法 方法验证 色谱定量分析
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Determination of anthraquinone prodrug and its hydrolytically active compounds using RP-HPLC
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作者 段艳冰 余佳 +1 位作者 刘海峰 吉民 《Journal of Southeast University(English Edition)》 EI CAS 2009年第1期128-131,共4页
In order to study the hydrolytic characterization of an anti-inflammatory prodrug ( RI-1 ) in vitro, an effective, accurate and reliable method for the simultaneous determination of the prodrug and its two hydrolyti... In order to study the hydrolytic characterization of an anti-inflammatory prodrug ( RI-1 ) in vitro, an effective, accurate and reliable method for the simultaneous determination of the prodrug and its two hydrolytic active compounds is developed using reverse phase high-performance liquid chromatography (RP-HPLC). The chromatographic separation is performed on an ODS-2 C18 column (250 mm × 4. 6 mm, 5.0 μm particle size) with a simple elution program. The mobile phase is V( methanol) : V(0. 1% phosphoric acid solution) =90:10 (adjust pH to 2. 3). A wavelength of 225 nm and a mobile phase flow rate of 1.0 mL/min are utilized for the quantitative analysis. Excellent linear behaviors over the investigated concentration ranges are observed with values of R2 higher than 0. 999 for all the analytes. The validated method is successfully applied to the simultaneous determination of the prodrug and its active components can be used to detect hydrolytic characterization in vitro. 展开更多
关键词 high-performance liquid chromatography (HPLC) quantitative analysis anthraquinone prodrug active components
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Analysis of phospholipids in microalga Nitzschia closterium by UPLC-Q-TOF-MS 被引量:6
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作者 严小军 李海英 +1 位作者 徐继林 周成旭 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第1期106-112,共7页
Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectr... Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts.Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode.Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode.The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode;the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances.As a result,18 lipid molecular species were identified for the first time in this microalga,comprising seven phosphatidylcholines (PC),two phosphatidylethanolamines (PE),two phosphatidylinositols (PI),and seven phosphatidylglycerols (PG).Lipid standards of PC,PE,PI,and PG were added to the total lipids as internal standards for semiquantitative analysis,revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg.This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time. 展开更多
关键词 Nitzschia closterium PHOSPHOLIPIDS UPLC-Q-TOF-MS
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Analysis of Nitrosamines in Organic Foods
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作者 L. Peykova D. Obreshkova I. Pencheva 《Journal of Chemistry and Chemical Engineering》 2011年第11期1014-1019,共6页
Nitrosamines are classified by IARC as Group 2B carcinogens. Usually they might be present in organic foods as products of reaction between secondary amines and nitrosation system. The aim of the study was to test the... Nitrosamines are classified by IARC as Group 2B carcinogens. Usually they might be present in organic foods as products of reaction between secondary amines and nitrosation system. The aim of the study was to test the concentration of nitrosamines in Bulgarian products. High performance liquid chromatography with UV detector was used for identification and quantitation. A standard solution of N-nitrosodiethanolamine was used as a reference substance and in the validation procedure of samples. The limit of detection of the method was determined to 14× 10^-9 g/mL. The results of the testing showed that analyzed organic foods produced in Bulgaria did not contain nitrosamines above the limit of detection of the method. 展开更多
关键词 HPLC organic foods NITROSAMINES N-nitrosodiethanolamine
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Qualitative and Quantitative Analysis of Linoleic Acid in Polygonati Rhizoma 被引量:1
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作者 ZHOU Zhun YUAN Han-Wen +8 位作者 PENG Cai-Yun JIAN Yu-Qing ZHOU Xu-Dong LI Bin SHENG Wen-Bing GONG Li-Min HE Shu-Jin WANG Wei LIU Chang-Xiao 《Digital Chinese Medicine》 2020年第3期180-187,共8页
Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently... Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精). 展开更多
关键词 Polygonati Rhizoma(Huang Jing 黄精) Linoleic acid Qualitative analysis Quantitative analysis Thin layer chromatography(TLC) High performance liquid chromatography(HPLC)
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Quantitative analysis of trace levels of β-ionone in water by liquid-liquidphase extraction-gas chromatography-mass spectrometry(LLE-GC-MS) 被引量:1
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作者 高梦鸿 高乃云 +3 位作者 谢茴茴 安娜 邓扬 戎文磊 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第2期472-477,共6页
A simple and rapid technique based on liquid-liquid extraction coupled to gas chromatography-mass spectrometric detection(LLE-GC-MS) was developed for analysis of taste and odour compound β-ionone in water. Instrumen... A simple and rapid technique based on liquid-liquid extraction coupled to gas chromatography-mass spectrometric detection(LLE-GC-MS) was developed for analysis of taste and odour compound β-ionone in water. Instrument parameters including programmed oven temperature, injection temperature and ion source temperature were evaluated and optimized. Effects of extraction time, ionic strength and p H on the detection efficiency were investigated and optimum conditions were 8 min of extraction time, without Na Cl addition at p H=9. Good linearity(R2=0.9997) was obtained when the linear range was 10-500 μg/L. The recoveries of β-ionone in ultrapure water and tap water samples were 88%-95% and 110%-114%, respectively. The relative standard deviations(RSD) were less than 10%. The method detection limit(MDL) and rejection quality level(RQL) were achieved at1.98 μg/L and 6.53 μg/L, respectively. LLE-GC-MS was demonstrated to be a rapid and convenient method for the determination ofβ-ionone in water samples. 展开更多
关键词 liquid-liquid extraction gas chromatography-mass spectrometry Β-IONONE WATER
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Principal Component Analysis (PCA) on Multivariate Data of Lard Analysis in Cooking Oil 被引量:1
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作者 Nor Aishah Mohd Salleh Mohd Sukri Hassan 《Journal of Mathematics and System Science》 2015年第7期300-306,共7页
Discrimination of fatty acids (FAs) of lard in used cooking oil is important in halal determination. The aim of this study was to find the information related to the changes FAs of lard when frying in cooking oil. Q... Discrimination of fatty acids (FAs) of lard in used cooking oil is important in halal determination. The aim of this study was to find the information related to the changes FAs of lard when frying in cooking oil. Quantitative analysis of FAs composition extracted from a series of experiments which involving frying cooking oil spiked with lard at three different parameters; concentration of spiked lard, heating temperatures and period of frying. The samples were analyzed using Gas Chromatography (GC) and Principal Components Analysis (PCA) technique. Multivariate data from chromatograms of FAs were standardized and computed using Unscrambler X10 into covariance matrix and eigenvectors correspond to Principal Components (PCs). Results have shown that the first and second PCs contribute to the FAs mapping which can be visualized by scores and loading plots to discriminate FAs of lard in used cooking oil 展开更多
关键词 Fatty acids LARD gas chromatography Principal Components Analysis
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芪葵颗粒定性定量方法研究 被引量:19
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作者 李爽 贾媛 +2 位作者 范玲 马兴苗 刘志辉 《药物分析杂志》 CAS CSCD 北大核心 2012年第9期1564-1568,1577,共6页
目的:建立芪葵颗粒的定性定量方法。方法:用薄层色谱法对该颗粒中的黄芪、黄蜀葵花、制何首乌进行定性鉴别。采用HPLC-ELSD法测定制剂中黄芪甲苷,使用Hedera ODS-2(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(32∶68)为流动相,流速1.0 m... 目的:建立芪葵颗粒的定性定量方法。方法:用薄层色谱法对该颗粒中的黄芪、黄蜀葵花、制何首乌进行定性鉴别。采用HPLC-ELSD法测定制剂中黄芪甲苷,使用Hedera ODS-2(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(32∶68)为流动相,流速1.0 mL.min-1,柱温30℃,Alltech 2000ES蒸发光散射检测器(漂移管温度102℃,载气流量2.8 L.min-1);采用HPLC法测定制剂中金丝桃苷、2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(简称二苯乙烯苷)的含量,使用HederaODS-2(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,流速1.0 mL.min-1,检测波长320 nm,柱温30℃。结果:在薄层色谱中均能检出黄芪、黄蜀葵花、制何首乌。黄芪甲苷、金丝桃苷、二苯乙烯苷线性范围分别为1.59~21.3μg(r=0.9997),0.048~0.567μg(r=0.9999),0.054~0.625μg(r=1.000);平均回收率(n=6)分别为95.0%(RSD=2.2%),104.0%(RSD=1.3%),101.5%(RSD=1.4%)。结论:所建方法简便、准确,重复性好,可作为复方芪葵颗粒的定性定量方法。 展开更多
关键词 中药制剂芪葵颗粒 黄芪 黄蜀葵花 制何首乌 黄芪甲苷 金丝桃苷 二苯乙烯苷 薄层定性鉴别 高效液相色谱定量分析
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Fast and simultaneous determination of ginsenosides by isocratic high-performance liquid chromatography
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作者 Da-Wei Lou 娄大伟 《Journal of Chinese Pharmaceutical Sciences》 CAS 2010年第5期393-399,共7页
A high-performance liquid chromatography method for the simultaneous determination of seven bioactive ginsenosides with diol stationary phase and isocratic elution was used to determin the ginsenosides in ginseng prod... A high-performance liquid chromatography method for the simultaneous determination of seven bioactive ginsenosides with diol stationary phase and isocratic elution was used to determin the ginsenosides in ginseng products. The optimization of the chromatographic separation was performed and the effect of temperature on separation was investigated. Using the validated procedure, the developed method was demonstrated to be more sensitive and effective than the conventional reversed-phase chromatography, where the chromatographic run is time-consuming to analyze a large number of ginsenosides. The results indicated that the developed method can be used for the quantitative determination of ginsenosides in complex ginseng samples. 展开更多
关键词 Liquid chromatography Pharmaceutical analysis Quantification GINSENOSIDES GINSENG
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Simultaneous quantification of lopinavir and ritonavir in human plasma by high performance liquid chromatography coupled with UV detection 被引量:1
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作者 KOU HuiJuan YE Min +5 位作者 FU Qiang HAN Yang DU XiaoLi XIE Jing ZHU Zhu LI TaiSheng 《Science China(Life Sciences)》 SCIE CAS 2012年第4期321-327,共7页
High performance liquid chromatography was coupled with UV detection for simultaneous quantification of lopinavir (LPV) and ritonavir (RTV) in human plasma. This assay was sensitive, accurate and simple, and only ... High performance liquid chromatography was coupled with UV detection for simultaneous quantification of lopinavir (LPV) and ritonavir (RTV) in human plasma. This assay was sensitive, accurate and simple, and only used 200μL of plasma sample. Samples were liquid-liquid extracted, and diazepam was used as an internal standard. The chromatographic separation was achieved on a C18 reversed-phase analytic column with a mobile phase of acetonitrile-sodium dihydrogen phosphate buffer (10 mmol L-1, pH 4.80) (60:40, v/v). UV detection was conducted at 205 nm and the column oven was set at 40℃. Calibration curves were constructed between 0,5-20 μg mL-1 for LPV and 0.05-5 μg mL-1 for RTV. The relative standard deviations were 2.16%-3.20% for LPV and 2.12%-2.60% for RTV for intra-day analysis, and 2.34%-4.04% for LPV and 0.31%-4.94% for RTV for inter-day analysis. The accuracy was within 100%+10%. The mean extraction recoveries were 79.17%, 52.26% and 91.35% for RTV, LPV and diazepam, respectively. This method was successfully applied to human plasma samples from patients orally administered a salvage regimen of lopinavir-ritonavir tablets. 展开更多
关键词 LOPINAVIR ritonavir HPLC UV detection human plasma
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