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人工神经网络法预测二维色谱柱效 被引量:11
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作者 黄俊 周申范 +1 位作者 国振双 许国旺 《色谱》 CAS CSCD 北大核心 2000年第1期1-4,共4页
采用基于变步长BP算法的人工神经网络,对高效微填充柱毛细管柱构成的二维柱色谱系统建立了柱效与影响因素的权接拓扑模型,并用于不同操作条件下二维柱系统的柱效预测中,取得了较好的效果。
关键词 二维 人工神经网络 BP算法 色谱柱效
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色谱柱效的评价 被引量:1
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作者 于峰 《浙江化工》 CAS 1991年第3期8-11,共4页
本文综评了色谱理论中的各种典型柱效参数,并对这些参数以及随之产生有密切关系的t_R与W_(1/2)之间的关系进行了一些比较和探讨。
关键词 色谱柱效
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一种新的色谱柱效评价方法-跃迁次数m评价法
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作者 李英杰 苏立强 王玫 《齐齐哈尔大学学报(自然科学版)》 2001年第2期1-2,共2页
依据色谱柱分离过程的驰豫理论 ,得出了色谱流出曲线方程及其一阶原点矩 ,二、三阶中心矩的表达式 ,验证了驰豫理论所提出的跃迁次数的意义 ,讨论了m与组分、半高峰宽、流动相速度的关系 。
关键词 驰豫理论 色谱柱效 跃迁次数
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基于柱效指数监测的双波长定量比率指纹图谱评价朱砂安神丸 被引量:2
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作者 孙国祥 尹霞 +1 位作者 高嘉悦 张建青 《中南药学》 CAS 2014年第9期833-836,共4页
目的建立色谱指纹图谱柱效指数模型,用以检测定量指纹图谱的变化。方法用高效液相色谱二极管阵列检测器同时测定12批朱砂安神丸(ZSASP)在203 nm和286 nm指纹谱(NFP)和比率指纹图谱(ROFP),分别以均值法整合同波长下指纹图谱,最后进行双... 目的建立色谱指纹图谱柱效指数模型,用以检测定量指纹图谱的变化。方法用高效液相色谱二极管阵列检测器同时测定12批朱砂安神丸(ZSASP)在203 nm和286 nm指纹谱(NFP)和比率指纹图谱(ROFP),分别以均值法整合同波长下指纹图谱,最后进行双波长指纹图谱的二次均值整合,计算双波长指纹图谱柱效指数并进行比较。结果以小檗碱(BBR)为参照物峰,确定57(203 nm)和50(286 nm)个共有指纹峰,建立了双波长ZSASP-HPLC指纹图谱和比率指纹图谱。首先将同波长下的NFP和ROFP进行均值整合,之后进行双波长二次均值整合鉴定出S1、S4、S5、S6、S9和S10质量极好(1级),S2、S3、S11和S12质量很好(2级),S7和S8质量次(7级)。在203 nm,S4、S5、S6、S9柱效指数较高,而在286 nm下四者柱效指数较低造成两者差值很大,S8呈现上述反变化过程造成较大差异值。结论色谱指纹图谱柱效指数能监测中药定量指纹图谱的柱效关联分离度后的变化情况,因此柱效指数标志着信号均化性和分离度均化性以及峰宽变动情况。 展开更多
关键词 指纹图指数 指纹图全分离指数 双波长定量比率指纹图 朱砂安神丸
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甲烷化微量一氧化碳和二氧化碳色谱分析方法的改进
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作者 尚杰峰 潘瑞华 韩萍 《大氮肥》 CAS 2016年第3期211-213,共3页
改进一种使用固定相Porapak-Q长4 m的不锈钢填充柱,选择适当的载气压力、温度等操作条件和主要参数的气相色谱分析方法,实现合成甲烷化气体样品中微量CO和CO_2的色谱分析。该分析方法基线稳定、组分分离完全、色谱柱效高、检测灵敏度好... 改进一种使用固定相Porapak-Q长4 m的不锈钢填充柱,选择适当的载气压力、温度等操作条件和主要参数的气相色谱分析方法,实现合成甲烷化气体样品中微量CO和CO_2的色谱分析。该分析方法基线稳定、组分分离完全、色谱柱效高、检测灵敏度好、定量准确,取得了良好的试验结果。 展开更多
关键词 微量CO、CO2 固定相 色谱柱效
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Polyphenols from Vitis thunbergii Sieb. et Zucc. 被引量:1
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作者 窦德强 任杰 +7 位作者 Maggie Cooper 何曜华 裴玉萍 Yoshiaki Takaya Masatake Niwa 陈英杰 姚新生 周仁平 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第2期57-59,共3页
Aim Isolation and structural elucidation of the constituents from the aerial part of Vitis thunbergii . Methods To isolate chemical constituents, column chromatography and HPLC were used. Physico chemical ... Aim Isolation and structural elucidation of the constituents from the aerial part of Vitis thunbergii . Methods To isolate chemical constituents, column chromatography and HPLC were used. Physico chemical characterization and spectroscopic analysis were employed for structural identification. Results Eleven polyphenols were isolated and identified. Conclusion Compound 1 is a new compound and its structure was characterized to be 3,5 dimethoxyl 4 hydroxyl phenylpropanol 9 O β D glycopyranoside. 展开更多
关键词 Vitis thunbergii phenylpropanol glycoside POLYPHENOL
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Determination of Lansoprazole by Direct Injection of Plasma and High Performance Liquid Chromatography with Column Switching 被引量:2
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作者 叶利民 李章万 +1 位作者 洪诤 钱广生 《Journal of Chinese Pharmaceutical Sciences》 CAS 1998年第4期30-33,共4页
High performance liquid chromatography with column switching has been developed for the determination of lansoprazole in plasma. The plasma samples were injected onto a pretreatment column packed with LiChromprep RP2 ... High performance liquid chromatography with column switching has been developed for the determination of lansoprazole in plasma. The plasma samples were injected onto a pretreatment column packed with LiChromprep RP2 (25~40 mm) after simple dilution with distilled water. Distilled water was used to wash out protein and other polar components in plasma. After switching, the concentrated lansoprazole was eluted in the backflush mode onto a Shimpack CLC ODS column with methanol 0 2 mol·L 1 ammonium acetate (65:35) as mobile phase. Purge solutions were used for clean up and for regenerating the pretreatment column. The method showed good precision and recovery. The detection limit was 0 005 mg·L -1 plasma. The RSD’s (intra and interday) were less than 2 5% and 5 3% respectively. The method has been successfully used to determine pharmacokinetics of lansoprazole in Chinese volunteers. 展开更多
关键词 LANSOPRAZOLE HPLC PHARMACOKINETICS Column switching technique
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Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC 被引量:6
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作者 张晶晶 张全斌 +2 位作者 王晶 石学连 张忠山 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第3期578-582,共5页
We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the m... We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaecharide derivatives, obtained with 1-Phenyl-3-methyl-5- pyrazolone (PMP), were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1-2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration (r^2〉0.998). The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans. 展开更多
关键词 FUCOIDAN MONOSACCHARIDE DERIVATION HPLC
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Combination of reversed phase liquid chromatography and zwitterion exchange-reversed phase-hydrophilic interaction mixed-mode liquid chromatography coupled with mass spectrometry for the analysis of antibiotics and their impurities 被引量:1
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作者 卢丽 李进 +1 位作者 金少鸿 胡昌勤 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2014年第2期106-117,共12页
In this study, a system involving two-dimensional, column-switching high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (LC-MS/MS) was developed and optimized for the analysis of an... In this study, a system involving two-dimensional, column-switching high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (LC-MS/MS) was developed and optimized for the analysis of antibiotics and their related substances. In the first-dimensional chromatography, the analytes were separated on a zwitterion exchange-reversed phase-hydrophitic interaction (ZIC-RP-HILIC) mixed-mode column coupled with tandem mass spectrometry (LC-MS/MS). A commonly used reversed phase LC column was employed in the second-dimensional chromatography. The mobile phase for the ZIC-RP-HILIC mixed-mode chromatography consisted of a volatile buffer that was compatible with LC-MS/MS, which enhanced the efficiency of ionization for structure elucidation. The antibiotic impurities eluted in the ion-pairing reversed phase chromatography were directly characterized by the ZIC-RP-HILIC-MS system, and the orthogonal separation of ZIC-RP-HILIC mixed-mode chromatography and reversed phase LC provided extra confidence that no impurity was missed. The efficiency of this method was demonstrated in the analysis of penicillin V potassium, oxacillin sodium, ceftriaxone sodium, and their impurities. In addition, this method is convenient for impurity profiling of antibiotics, and may be used for the analysis of other pharmaceutical ingredients. 展开更多
关键词 Impurity profiling Two-dimensional chromatography Colunm-switching Mixed-mode chromatography LC/MSm
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