High Performance Liquid Chromatography with Fluorescence Detection (HPLC-FD) and Mass Spectrometry (MS) have been used to analyze the purified Gonyautoxin2,3 (GTX2,3) and Gonyautoxin1,4 (GTX1,4), the compound in the f...High Performance Liquid Chromatography with Fluorescence Detection (HPLC-FD) and Mass Spectrometry (MS) have been used to analyze the purified Gonyautoxin2,3 (GTX2,3) and Gonyautoxin1,4 (GTX1,4), the compound in the family of toxins responsible for paralytic shellfish poisoning (PSP), from a strain of the dinoflagellate Alexandrium tamarense. The results showed that the retention time of the purified GTX2,3 and the purified GTX1,4 was the same as that of the standard GTX2,3 and the standard GTX1,4 by the HPLC method. Combining the purified GTX2,3 and the purified GTX1,4 showed the typical poisoning symptom of PSP toxins by mouse bioassay, the author thought the purified toxins were GTX2,3 and GTX1,4. The positive ionization produced the typical [M+H]+ molecular ion of the purified GTX2,3 and the purified GTX1,4 (m/z 396 and 412), which was the same as that of the standard GTX2,3 and the standard GTX1,4 by MS method. The fragment ions of the purified GTX2,3 (m/z 378, 316, 298) and the purified GTX1,4 (m/z 394, 332, 314), generated by Collision Induced Dissociations (CID) of their respective [M+H]+ ions, were also identical to those obtained for the standard GTX2,3 and the standard GTX1,4. They were further confirmed that the purified toxins were GTX2,3 and GTX1,4. Of course, the Nuclear Magnetic Resonance (NMR) and Infrared (IR) are needed to fully confirm the results.展开更多
文摘High Performance Liquid Chromatography with Fluorescence Detection (HPLC-FD) and Mass Spectrometry (MS) have been used to analyze the purified Gonyautoxin2,3 (GTX2,3) and Gonyautoxin1,4 (GTX1,4), the compound in the family of toxins responsible for paralytic shellfish poisoning (PSP), from a strain of the dinoflagellate Alexandrium tamarense. The results showed that the retention time of the purified GTX2,3 and the purified GTX1,4 was the same as that of the standard GTX2,3 and the standard GTX1,4 by the HPLC method. Combining the purified GTX2,3 and the purified GTX1,4 showed the typical poisoning symptom of PSP toxins by mouse bioassay, the author thought the purified toxins were GTX2,3 and GTX1,4. The positive ionization produced the typical [M+H]+ molecular ion of the purified GTX2,3 and the purified GTX1,4 (m/z 396 and 412), which was the same as that of the standard GTX2,3 and the standard GTX1,4 by MS method. The fragment ions of the purified GTX2,3 (m/z 378, 316, 298) and the purified GTX1,4 (m/z 394, 332, 314), generated by Collision Induced Dissociations (CID) of their respective [M+H]+ ions, were also identical to those obtained for the standard GTX2,3 and the standard GTX1,4. They were further confirmed that the purified toxins were GTX2,3 and GTX1,4. Of course, the Nuclear Magnetic Resonance (NMR) and Infrared (IR) are needed to fully confirm the results.
