高效液相色谱法分析色酒中有机酸的研究

酒中有机酸的种类、含最和构成不同,形成酒的各种风味和品质。适量的有机酸能促进食欲、助消化,益于健康。因而酒中有机酸的分析对酒的品评很有意义。

固相萃取-反相HPLC分析色酒中有机酸

以高效液相色谱法对三种有色酒中的七种有机酸进行了分析。采用 C18预处理柱进行样品前处理 ,固定相为 μBondapak C18柱 ,流动相为 p H2 .9的 5g· L-1磷酸氢二铵溶液 ,流速为1 ml· min-1,检测波长为 2 1 4nm,方法回收率为 96%～ 1 1 3% ,检出限为 2 0～ 1 2

浓浓乡色酒 悠悠思乡情——读舒兰《乡色酒》

半个多世纪以来，那“一湾浅浅的海峡（余光中《乡愁》）”，汇聚了多少思乡泪，隔断了多少血脉情。乡愁，是一种美丽的传染病，深深地感染了台岛的每一个有识之士。舒兰的《乡色酒》，道出了多少游子的心声。

一曲深情而优美的乡愁绝唱——《乡色酒》赏析

这是一首短诗，与余光中的《乡愁》相比，写得同样优秀。全诗总共两下十行，却别出心裁，诗味隽永。诗人把乡愁写得缠绵悱恻，令人唏嘘感叹。上节写三十年前，那时，月圆人也圆；下节写三十年后，这时，月满乡愁也满。

台湾乡愁诗两首赏析

自李白“举头望明月，低头思故乡”之后，诗人喜欢向月亮倾诉远离故土的乡愁。台湾诗人舒兰的《乡色酒》和李春生的《无月的望》也是同一题材。

诗以根土秀 月是故乡明——论舒兰的乡情书写

在当代华语诗坛上,舒兰以其对祖国大陆故乡的书写而留下颇具特色的身影,其诗作少而精,但学界对其缺乏应有的关注。本文以舒兰的诗集为考察文本,以故乡的书写为研究对象,从人伦之思、山水之思、家国之思三方面探讨舒兰浓郁乡情书写中的思乡情结,试图走进舒兰"掉头一去是风吹黑发,回首再来已雪满白头"的情感世界,挖掘其诗歌文本中深厚的家国情怀。

Analysis of Sugar Components of Fermented Rice Wine by Ion Chromatography

[Objective] The aim was to analyze sugar components in fermented rice wine by ion chromatography. [Method] The optimal condition for chromatography system of sugar analysis was selected by measuring sugars in fermented rice wine with ion chromatography and pulsed amperometric detection. [Result] The optimal measurement conditions were as follows： Leacheate （Leachate）, consisting of NaOH and CH3COONa, was eluted by gradient concentrations, with column temperature at 35 ℃ and flow rate at 0.4 ml/min. In the condition, sugars in rice wine were ana- lyzed and the results showed that the method is featured by low detection limit, good repetition and high recovery rate. [Conclusion] The research establishes and determines the approaches and optimum conditions for sugar analysis in rice wine by ion chromatography and pulsed amperometric detection, providing references for advancement of research on quality improvement of fermented rice wine.

Experiments on decolourization with 122-resin for extraction of gib-berellins from solid medium under different conditions

The extracting liquid of gibberellins (Gibberella fujikuroi) from solid medium for was decolorized separately with 75%, 95% alcohol, and distilled water in static adsorption and vibrating way for different durations. The results showed that the content of GA3 in efflux extracted with alcohol is 10% higher than that with distilled water. With the increase of the durations of extraction, the content of GA3 increases and the dissolution of pigments also increase. For extraction of GA3 cultured in solid medium, the best decolorizing result was obtained when it was extracted with 75%-95% alcohol in static way for 1-3 h, or in a vibrating way for 30-60 min, and then to decolor in a static way for 30-60 min in 122 resin column.

Role of iron in hepatic fibrosis: One piece in the puzzle

Iron is an essential element involved in various biological pathways. When present in excess within the cell, iron can be toxic due to its ability to catalyse the formation of damaging radicals, which promote cellular injury and cell death. Within the liver, iron related oxidative stress can lead to fibrosis and ultimately to cirrhosis. Here we review the role of excessive iron in the pathologies associated with various chronic diseases of the liver. We also describe the molecular mechanism by which iron contributes to the development of hepatic fibrosis.

Genome-wide differences in hepatitis C-vs alcoholism-associated hepatocellular carcinoma

AIM:To look at a comprehensive picture of etiology- dependent gene abnormalities in hepatocellular carcinoma in Western Europe. METHODS:With a liver-oriented microarray,transcript levels were compared in nodules and cirrhosis from a training set of patients with hepatocellular carcinoma (alcoholism,12;hepatitis C,10)and 5 controls.Loose or tight selection of informative transcripts with an abnormal abundance was statistically valid and the tightly selected transcripts were next quantified by qRTPCR in the nodules from our training set(12+10) and a test set(6+7). RESULTS:A selection of 475 transcripts pointed to significant gene over-representation on chromosome 8 (alcoholism)or-2(hepatitis C)and ontology indicated a predominant inflammatory response(alcoholism)or changes in cell cycle regulation,transcription factors and interferon responsiveness(hepatitis C).A stringent selection of 23 transcripts whose differences betweenetiologies were significant in nodules but not in cirrhotic tissue indicated that the above dysregulations take place in tumor but not in the surrounding cirrhosis.These 23 transcripts separated our test set according to etiologies. The inflammation-associated transcripts pointed to limited alterations of free iron metabolism in alcoholic vs hepatitis C tumors. CONCLUSION:Etiology-specific abnormalities(chromo- some preference;differences in transcriptomes and related functions)have been identified in hepatocellular carcinoma driven by alcoholism or hepatitis C.This may open novel avenues for differential therapies in this disease.

Hepatoprotective and antioxidant effects of lycopene on non-alcoholic fatty liver disease in rat

AIM To evaluate the hepatoprotective effect of lycopene(Ly) on non-alcoholic fatty liver disease(NAFLD) in rat. METHODS A rat model of NAFLD was first established by feeding a high-fat diet for 14 wk. Sixty-five rats were randomly divided into normal group, model group and Ly treatment groups. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), triglycerides(TG), total cholesterol(TC) in serum and low density lipoproteincholesterol(LDL-C), high density lipoprotein-cholesterol(HDL-C), free fatty acid(FFA), malondialdehyde(MDA), superoxide dismutase(SOD), glutathione(GSH) in liver tissue were evaluated, respectively. While the hepatoprotective effect was also confirmed by histopathological analysis, the expression levels of TNF-α and cytochrome P450(CYP) 2E1 in rat liver were determined by immunohistochemistry analysis.RESULTS A significant decrease was observed in the levels of serum AST(2.07-fold), ALT(2.95-fold), and the blood lipid TG(2.34-fold) and TC(1.66-fold) in the dose of 20 mg/kg Ly-treated rats(P < 0.01), compared to the model group. Pretreatment with 5, 10 and 20 mg/kg of Ly significantly raised the levels of antioxidant enzyme SOD in a dose-dependent manner,to 90.95 ± 9.56, 109.52 ± 11.34 and 121.25 ± 10.68(P < 0.05, P < 0.01), as compared with the model group. Similarly, the levels of GSH were significantly increased(P < 0.05, P < 0.01) after the Ly treatment. Meanwhile, pretreatment with 5, 10 and 20 mg/kg of Ly significantly reduced MDA amount by 30.87, 45.51 and 54.49% in the liver homogenates, respectively(P < 0.01). The Ly treatment group showed significantly decreased levels of lipid products LDL-C(P < 0.05, P < 0.01), improved HDL-C level and significantly decreased content of FFA, compared to the model group(P < 0.05, P < 0.01). Furthermore, the Ly-treated group also exhibited a down-regulated TNF-α and CYP2E1 expression, decreased infiltration of liver fats and reversed histopathological changes, all in a dosedependent manner(P < 0.05, P < 0.01). CONCLUSION This study suggests that Ly has a protective effect on NAFLD, down-regulates expression of TNF-α, and that CYP2E1 may be one of the action mechanisms for Ly.

FLASHLAMP- PUMPED PULSED DYE LASER IN TREATMENT OF PORT- WINE STAINS

To assess the effectiveness of the flashlamp- pumped pulsed dye laser (Photogeneca V, Synosure Corp, Boston, United States) in the treatment of port- wine stains. Methods. One hundred and ninety- four consecutive patients with port- wine stains were treated with a flashlamp- pumped pulsed dye laser in Peking Union Medical College Hospital from January 1998 to August 1999. Results. Of 194 patients who completed treatment, 56.2％ had more than 60％ fading of the lesion and only 6.7％ had less than 20％ fading. An average of 3.6 treatments were needed to achieve more than 60％ fading. The response was better in children than in adults, although the difference was not significant. Pigmentary change (usually transient) occurred in 3.1％ of patients. Conclusions. This study confirms the efficacy of the flashlamp- pumped pulsed dye laser in the treatment of port- wine stains in children and adults.

Mechanical Circulatory Support in Patients with Sepsis： A Case Series

MCS （mechanical circulatory support） has shown great efficacy in treatment of respiratory mid cardiovascular diseases when conventional therapy is insufficient. The use of ECMO （extracolporeal membrane oxygenation） for the treatment of cardiogenic shock secondary to sepsis is still controversial. Studies have shown that ECMO should be considered as a valuable therapeutic option in cases of refractory cardiovascular dysfunction in severe septic patients, although more studies ale needed to consolidate its efficacy. We report the case of two patients, without previous history of cardiopathy, subjected to temporary MCS, with use of ECMO for the treatment of refractory cardiogenic shock secondary to sepsis.