节头虫属(Genus Arthricocephalus)的概念一直较为混乱,在为它的模式种乔氏节头虫(Arthricocephalus chauveaui)指定"选模"后,又进一步加剧。经对A.chauveaui的原始手标本上保存的所有三叶虫研究后,发现命名者Bergeron所附A.c...节头虫属(Genus Arthricocephalus)的概念一直较为混乱,在为它的模式种乔氏节头虫(Arthricocephalus chauveaui)指定"选模"后,又进一步加剧。经对A.chauveaui的原始手标本上保存的所有三叶虫研究后,发现命名者Bergeron所附A.chauveaui的唯一图片,依据的是其中一枚背壳,特征吻合,从而认定该背壳是A.chauveaui的正模,代表了模式种和节头虫属的概念,是该种和该属名称的载体。也表明为A.chauveaui建立"选模",既无必要,也违背命名者的本意。而且选择一个不归属于A.chauveaui的标本来代表该种、该属的概念,就更不妥当。经研究,手标本上的三叶虫不是同一个种,而是隶属于三个不同种,这三个种又应归属于三个不同的属,即Arthricocephalus,Oryctocarella和Duyunaspis。A.chauveaui"选模"头盖并不属于该种,应归属于Oryctocarella duyunensis。研究还表明,有三个分类单元是Arthricocephalus的晚出异名,即Arthricocephalus(Arthricocephalites)Chien et Lin in Lu et al.,1974,Haliplanktes Blaker et Peel,1997,和Arthricocephalites Chien et Lin sensu Yuan et al.,2002。展开更多
AIM： To examine the effects of 10% diluted honey, which has been shown to be scolicidal, on the liver and biliary system and determine whether it could be used as a scolicidal agent in the presence of biliary-cystic ...AIM： To examine the effects of 10% diluted honey, which has been shown to be scolicidal, on the liver and biliary system and determine whether it could be used as a scolicidal agent in the presence of biliary-cystic communication. METHODS： Thirty Wistar-Albino rats were divided into two groups. Honey with 10% dilution in the study group and 0.9% saline （NaCI） in the control group were injected into the common bile ducts of rats through a 3-mm duodenotomy. The animals were sacrificed 6 mo alter the procedure. Histopathological, biochemical, and radiological examinations were performed for evaluation of side effects. RESULTS： At the end of the sixth month, liver function tests were found to be normal in both groups. The tissue samples of liver and ductus choledochus of the honey group showed no histomorphologic difference from the control group. No stricture on the biliary tree was detected on the retrograde cholangiograms. CONCLUSION： According to these results, we concluded that 10% diluted honey could be used as scolicidal agent safely in the presence of biliary-cystic communication.展开更多
Colorectal cancer screening can be performed by fecal occult blood testing, sigmoidoscopy, double contrast barium enema or colonoscopy. Colonoscopy has the significant advantage that polyps can be removed during the s...Colorectal cancer screening can be performed by fecal occult blood testing, sigmoidoscopy, double contrast barium enema or colonoscopy. Colonoscopy has the significant advantage that polyps can be removed during the same procedure. Occasionally, colonoscopy can also reveal unexpected findings, including parasitic infections, even in asymptomatic patients. Tapeworms or cestodes are hermaphroditic parasites, which can live for considerable periods of time in the human gastrointestinal tract. Fish tapeworm (Diphyllobothrium species) are endemic in various parts of the world, the commonest species being Diphyllobothrium latum. Humans are the main definitive host for D. Latum and the majority of individuals harbouring the parasite are asymptomatic, while 40% of infected individuals may have low vitamin B12 levels. We describe a case of D. latum infection found on routine colonoscopic screening for colorectal cancer in an asymptomatic patient, which was successfully treated with praziquantel. The infection likely arose following raw fish (sushi) consumption.展开更多
Objective To construct a λgt11 cDNA expression library of Echinococcus multilocularis protoscolex isolated in China. Methods Echinococcus multilocularis protoscolex mRNA was extracted using a Quickprep MicromRNA pu...Objective To construct a λgt11 cDNA expression library of Echinococcus multilocularis protoscolex isolated in China. Methods Echinococcus multilocularis protoscolex mRNA was extracted using a Quickprep MicromRNA purification kit based on combining of the disruptive and protective properties of guanidinium thiocyanate (GTC) with the speed and selectivity of oligo (dT)-cellulose chromatography in a spum-column with some modification. Purified mRNA (1.8 μg) was submitted to reverse transcription using random hexamers ［pd(N6)］. The double-strand blunt-ended cDNAs were ligated with an EcoRI/NotI adaptor to form a cohesive EcoRI end. Subsequently the synthesized cDNA was inserted into vector λgt11 EcoRI arms. After being packaged in vitro, λgt11 was put to an infectious bacteria Echinococcus coli (E.coli) strain Y1090; the recombinants were screened by color selection. PCR amplification was performed to evaluate the size of insertion DNA fragments.Results The recombinant ratio was nearly 100% and approximately 1×106 clones could be derived from this λgt11 cDNA library. PCR results indicated that the insertion DNAs were about 1.48 kb. Conclusions A λgt11 cDNA expression library consisting of a million recombinant clones has been constructed from Echinococcus multicularis protoscolex mRNA. Further studies on this library are deserved.展开更多
文摘节头虫属(Genus Arthricocephalus)的概念一直较为混乱,在为它的模式种乔氏节头虫(Arthricocephalus chauveaui)指定"选模"后,又进一步加剧。经对A.chauveaui的原始手标本上保存的所有三叶虫研究后,发现命名者Bergeron所附A.chauveaui的唯一图片,依据的是其中一枚背壳,特征吻合,从而认定该背壳是A.chauveaui的正模,代表了模式种和节头虫属的概念,是该种和该属名称的载体。也表明为A.chauveaui建立"选模",既无必要,也违背命名者的本意。而且选择一个不归属于A.chauveaui的标本来代表该种、该属的概念,就更不妥当。经研究,手标本上的三叶虫不是同一个种,而是隶属于三个不同种,这三个种又应归属于三个不同的属,即Arthricocephalus,Oryctocarella和Duyunaspis。A.chauveaui"选模"头盖并不属于该种,应归属于Oryctocarella duyunensis。研究还表明,有三个分类单元是Arthricocephalus的晚出异名,即Arthricocephalus(Arthricocephalites)Chien et Lin in Lu et al.,1974,Haliplanktes Blaker et Peel,1997,和Arthricocephalites Chien et Lin sensu Yuan et al.,2002。
文摘AIM： To examine the effects of 10% diluted honey, which has been shown to be scolicidal, on the liver and biliary system and determine whether it could be used as a scolicidal agent in the presence of biliary-cystic communication. METHODS： Thirty Wistar-Albino rats were divided into two groups. Honey with 10% dilution in the study group and 0.9% saline （NaCI） in the control group were injected into the common bile ducts of rats through a 3-mm duodenotomy. The animals were sacrificed 6 mo alter the procedure. Histopathological, biochemical, and radiological examinations were performed for evaluation of side effects. RESULTS： At the end of the sixth month, liver function tests were found to be normal in both groups. The tissue samples of liver and ductus choledochus of the honey group showed no histomorphologic difference from the control group. No stricture on the biliary tree was detected on the retrograde cholangiograms. CONCLUSION： According to these results, we concluded that 10% diluted honey could be used as scolicidal agent safely in the presence of biliary-cystic communication.
文摘Colorectal cancer screening can be performed by fecal occult blood testing, sigmoidoscopy, double contrast barium enema or colonoscopy. Colonoscopy has the significant advantage that polyps can be removed during the same procedure. Occasionally, colonoscopy can also reveal unexpected findings, including parasitic infections, even in asymptomatic patients. Tapeworms or cestodes are hermaphroditic parasites, which can live for considerable periods of time in the human gastrointestinal tract. Fish tapeworm (Diphyllobothrium species) are endemic in various parts of the world, the commonest species being Diphyllobothrium latum. Humans are the main definitive host for D. Latum and the majority of individuals harbouring the parasite are asymptomatic, while 40% of infected individuals may have low vitamin B12 levels. We describe a case of D. latum infection found on routine colonoscopic screening for colorectal cancer in an asymptomatic patient, which was successfully treated with praziquantel. The infection likely arose following raw fish (sushi) consumption.
文摘Objective To construct a λgt11 cDNA expression library of Echinococcus multilocularis protoscolex isolated in China. Methods Echinococcus multilocularis protoscolex mRNA was extracted using a Quickprep MicromRNA purification kit based on combining of the disruptive and protective properties of guanidinium thiocyanate (GTC) with the speed and selectivity of oligo (dT)-cellulose chromatography in a spum-column with some modification. Purified mRNA (1.8 μg) was submitted to reverse transcription using random hexamers ［pd(N6)］. The double-strand blunt-ended cDNAs were ligated with an EcoRI/NotI adaptor to form a cohesive EcoRI end. Subsequently the synthesized cDNA was inserted into vector λgt11 EcoRI arms. After being packaged in vitro, λgt11 was put to an infectious bacteria Echinococcus coli (E.coli) strain Y1090; the recombinants were screened by color selection. PCR amplification was performed to evaluate the size of insertion DNA fragments.Results The recombinant ratio was nearly 100% and approximately 1×106 clones could be derived from this λgt11 cDNA library. PCR results indicated that the insertion DNAs were about 1.48 kb. Conclusions A λgt11 cDNA expression library consisting of a million recombinant clones has been constructed from Echinococcus multicularis protoscolex mRNA. Further studies on this library are deserved.
