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毕克齐长山药节间组织培养及增殖的研究
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作者 白瑞琴 石岭 +3 位作者 陈贵华 杨玉林 赵卫国 王秀云 《中国蔬菜》 北大核心 2006年第B10期47-49,共3页
以毕克齐长山药节间茎段为外植体进行组织培养,探讨不同激素、N素处理对不定芽的形成及继代增殖的影响。结果表明:0.1mg·L^-1以下低浓度的6-BA对不定芽的形成有促进作用,其中MS+6-BA0.1mg·L^-1+NAA0.01mg·L^-1... 以毕克齐长山药节间茎段为外植体进行组织培养,探讨不同激素、N素处理对不定芽的形成及继代增殖的影响。结果表明:0.1mg·L^-1以下低浓度的6-BA对不定芽的形成有促进作用,其中MS+6-BA0.1mg·L^-1+NAA0.01mg·L^-1为适宜培养基,不定芽的形成率达75%;不定芽增殖适宜培养基为MS+6-BA2.0mg·L^-1+NAA0.5mg·L^-1,平均每个外植体再生芽数7.6个;培养基MS+IBA0.5mg·L^-1生根率可达100%。 展开更多
关键词 毕克齐长山药 节间组织培养 增殖
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毛竹快速拔节过程节间组织cDNA文库的构建与分析 被引量:2
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作者 郑丽娜 周明兵 《福建林业科技》 北大核心 2012年第3期19-23,共5页
以同一棵毛竹笋上快速伸长生长的节间组织和基本停止伸长生长的节间组织为材料,分别提取总RNA,纯化成mR-NA后合成cDNA双链;以Uni-ZAP XR vector为载体,构建了2个cDNA文库。快速伸长生长的、停止伸长生长的节间组织cDNA文库的滴度分别为9... 以同一棵毛竹笋上快速伸长生长的节间组织和基本停止伸长生长的节间组织为材料,分别提取总RNA,纯化成mR-NA后合成cDNA双链;以Uni-ZAP XR vector为载体,构建了2个cDNA文库。快速伸长生长的、停止伸长生长的节间组织cDNA文库的滴度分别为9.7×109、8.4×109pfu.mL-1,含插入片段的频率均达到99%以上,插入片段的大小均在500~3000 bp。cDNA文库的成功构建为探究毛竹快速生长的分子机理奠定了基础。 展开更多
关键词 毛竹 节间组织 CDNA文库
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蔗糖分子完整地进入甘蔗节间贮藏组织的证据
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作者 Ling.,SE 何明 《国外农学(甘蔗)》 1990年第2期24-26,共3页
关键词 甘蔗 蔗糖 贮藏组织
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Analyses of influence of residential buildings' space organization on heating energy consumption in Lhasa 被引量:7
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作者 Li En 《Journal of Southeast University(English Edition)》 EI CAS 2017年第4期457-465,共9页
The heating load simulation models of the residential buildings in Lhasa are established for enhancing the space organization’s adaptability to climate and radiation and improving its energy saving performance.The sp... The heating load simulation models of the residential buildings in Lhasa are established for enhancing the space organization’s adaptability to climate and radiation and improving its energy saving performance.The space organization items a e analyzed for both the existing buildings without insulation and new buildings with good insulation.The items include orientation design,south a d north balcony design,the north and south partition wall’s position design,storey height design and window-wall ratio design.Simulation results show that orientation is the key design element for energy saving design,and adverse orientation can obviouslyincrease heating energy consumption;south and north balconies can reduce winter heating energy consumption;partition walls move to the north,which means that the south room’s big depth design leads to less heating energy consumption,but the effect is not inconspicuous;smaier storey height results in less heating load.For the existing buildings,the window-wall ratio of south side has a balance point for energy saving design in the calculation condition.For the new buildings with good insulation,enlarging the south window-wal ratio can continuously reduce heating energy consumption,but the energy saving rate between models gets smaier.The heating energy consumption comparison study between the common model and optimal space design model demonstrates that the energy saving design can significantly reduce heating energy consumption 展开更多
关键词 residential building space organization heating energy consumption energy saving design
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The effect of spleen on the change of Treg cells during mouse pregnancy
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作者 GAO Qing LI Mu +3 位作者 ZHANG Shu ZHOU Rui WU Jinfang LI Zongfang 《Journal of Medical Colleges of PLA(China)》 CAS 2013年第5期273-280,共8页
Objective:To analyze the effect of spleen on the Treg cells during pregnancy. Methods: The mononuclear cells were separated from the peripheral, spleen and uterus or placenta blood. Flow cytometry was employed to anal... Objective:To analyze the effect of spleen on the Treg cells during pregnancy. Methods: The mononuclear cells were separated from the peripheral, spleen and uterus or placenta blood. Flow cytometry was employed to analyze the percent of Treg cells in total T cells in different stages of pregnancy. Immunohistochemical staining was used to make sure the distribution of Treg in spleen in different stages of pregnancy. Results: The results of immunohistochemical staining showed that compared with spleen Treg cells in normal unpregnant mice, spleen Treg cells on day 7 and 14 of pregnancy significantly increased. After splenectomy, peripheral blood and placenta Treg cells on day 7 of pregnancy markedly decreased as compared with the normal pregnancy(P<0.01). And the cells on day 14of pregnancy were markedly recovered as compared with the normal pregnancy. Conclusion: Our study indicated that the spleen and its Treg cells might play important roles in transient tolerance during pregnancy. 展开更多
关键词 SPLEEN Treg cells SPLENECTOMY PREGNANCY MOUSE
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