Objective： To explore the construction of metastatic spinal cancer （MSC） tissue microarrays and validate its value in immunohistochemical study of MSC. Methods： Paraffin-embedded specimens from 71 MSC cases and 6 ...Objective： To explore the construction of metastatic spinal cancer （MSC） tissue microarrays and validate its value in immunohistochemical study of MSC. Methods： Paraffin-embedded specimens from 71 MSC cases and 6 primary tumor cases were selected as donor blocks and prepared into MSC tissue microarrays by tissue array arrangement, the steps of which included location, punching, sampling, sample seeding, and re-diagnosis by hematoxylin-eosin （HE） as well as MMP-9 and MMP-14 immunohistoehemical staining. Results： The MSC tissue microarrays thus constructed were intact and craekless, containing 154 complete and well arranged microarray points. None of the sectioned tissue microarrays was lost, and the results of HE staining was consistent with the primary pathologic diagnoses. Immunohistochemical staining was also good without non-specific or marginal effect. Conclusion： The MSC tissue microarrays have a high value in the immunohistochemical study of MSC.展开更多
In this paper, an electrohydrodynamic approach is used to model and study dynamics of evaporating microdroplets in digital microfluidic systems. A numerical eleetrohydrodynamic approach is used to calculate the drivin...In this paper, an electrohydrodynamic approach is used to model and study dynamics of evaporating microdroplets in digital microfluidic systems. A numerical eleetrohydrodynamic approach is used to calculate the driving force and shear force （due to the walls）. Effects of contact line pinning is considered by adding a three-phase contact line force, and also considering dynamic contact angle which modifies the mierodroplet boundary conditions. Since air is used as the filler fluid, the drag force is neglected. Although energy equation is not solved （constant temperature assumption）, effects of the evaporation is considered from two aspects： It is shown that an additional force is needed to balance the dynamic equation of the mierodroplet motion. Also, at each time step the microdroplet interface has to be deformed due to the change in the microdroplet radius. Important findings of the proposed model includes the transient velocity and displacement of the microdroplet as well as the driving and opposing forces acting on the microdroplet as functions of time. It is shown that mass loss due to evaporation tends to accelerate the droplet; whereas the competitive effect of the reduced driving force decelerates the droplet at the end of motion. The modeling results indicate that evaporation plays a crucial role in microdroplet motion by changing the force balance and the microdroplet boundary condition.展开更多
基金supported by the research funds of Changzheng Hospital,Shanghai,Chinathe research funds of health bureau in Shanghai (320.2745)medical and health science research program of People’s Liberation Army in the eleventh 5-year plan (06MB218)
文摘Objective： To explore the construction of metastatic spinal cancer （MSC） tissue microarrays and validate its value in immunohistochemical study of MSC. Methods： Paraffin-embedded specimens from 71 MSC cases and 6 primary tumor cases were selected as donor blocks and prepared into MSC tissue microarrays by tissue array arrangement, the steps of which included location, punching, sampling, sample seeding, and re-diagnosis by hematoxylin-eosin （HE） as well as MMP-9 and MMP-14 immunohistoehemical staining. Results： The MSC tissue microarrays thus constructed were intact and craekless, containing 154 complete and well arranged microarray points. None of the sectioned tissue microarrays was lost, and the results of HE staining was consistent with the primary pathologic diagnoses. Immunohistochemical staining was also good without non-specific or marginal effect. Conclusion： The MSC tissue microarrays have a high value in the immunohistochemical study of MSC.
文摘In this paper, an electrohydrodynamic approach is used to model and study dynamics of evaporating microdroplets in digital microfluidic systems. A numerical eleetrohydrodynamic approach is used to calculate the driving force and shear force （due to the walls）. Effects of contact line pinning is considered by adding a three-phase contact line force, and also considering dynamic contact angle which modifies the mierodroplet boundary conditions. Since air is used as the filler fluid, the drag force is neglected. Although energy equation is not solved （constant temperature assumption）, effects of the evaporation is considered from two aspects： It is shown that an additional force is needed to balance the dynamic equation of the mierodroplet motion. Also, at each time step the microdroplet interface has to be deformed due to the change in the microdroplet radius. Important findings of the proposed model includes the transient velocity and displacement of the microdroplet as well as the driving and opposing forces acting on the microdroplet as functions of time. It is shown that mass loss due to evaporation tends to accelerate the droplet; whereas the competitive effect of the reduced driving force decelerates the droplet at the end of motion. The modeling results indicate that evaporation plays a crucial role in microdroplet motion by changing the force balance and the microdroplet boundary condition.
