花叶绿萝的多倍体诱导及快速繁殖

为改良现有的绿萝栽培品种,丰富遗传育种资源。在无菌条件下,将丛生芽浸入0.05%,0.1%,0.2%,0.5%秋水仙素水溶液中1d,然后转入MS+3mg·L$C16-BA+0.2mg·L$C1NAA中培养。培养30d后,4种处理都诱导出了变异芽,通过根尖细胞染色体检测,变异芽为四倍体或嵌合体,其中以浓度为0.2%的秋水仙素溶液浸泡处理效果最好,得到的变异芽71%为纯合的四倍体,其染色体数量由58(2n=2x=58)变为116(2n=4x=116)。

花叶绿萝组培快繁技术研究

以花叶绿萝带芽茎段为外植体,MS为基本培养基,探讨不同灭菌时间对外植体成活的影响;不同质量浓度激素组合对愈伤组织诱导及其分化、不定芽诱导、生根的影响.结果表明:带芽茎段可产生愈伤组织和不定芽,诱导愈伤组织的最适培养基为MS+6-BA2.0 mg/L+NAA 0.7 mg/L+2,4-D 0.3 mg/L,诱导愈伤组织分化的最佳培养基为MS+6-BA 2.0 mg/L+IBA 1.0 mg/L+KT 1.0 mg/L,最适的生根培养基为1/2 MS+NAA 0.1 mg/L.

几种名贵观赏植物的快速繁殖

通过研究不同生长素和细胞分裂素组合配比对火鹤、红宝石喜林芋、花叶绿萝增殖、分化和生根的影响，确定了这3种植物的最佳继代、壮苗和生根培养基，并针对火鹤选择出了优良的快繁无性系作为快繁材料。试管苗移栽驯化试验结果表明，只有在基质温度达15℃时试管苗才能正常生长，移栽结果还确定了这3种植物适宜的移栽基质。瓶外生根结果说明以上3种植物皆可在瓶外生根。

Determination and Comparison of Pigment Contents in Leaves of Punica granatum L. Hybrids with Different Leaf Colors

[Objective] This study aimed to investigate the reason for the different leaf colors of pomegranate hybrid progeny. [Method] Spectrophotometry was used to determine the contents of three pigments, including chlorophyll, carotenoid and anthocyanin, in the leaves of eight pomegranate individuals and their parents at growth stage. [Result] The results showed that during the early growth stage, the anthocyanin contents of four pomegranate individuals with red leaves were higher than the control, to be specific, the anthocyanin content of No.5 pomegranate individual reached the highest of 4.12 mg/g; the chlorophyll and carotenoid contents of four pomegranate individuals with yellow-green leaves showed consistent dynamic trends with the control, to be specific, the chlorophyll content and chlorophyll/carotenoid ratio of No.4 pomegranate individual reached the highest, while the chlorophyll a/carotenoid b ratio of No.3 pomegranate individual was the lowest. During vigorous growth stage, the contents of chlorophyll, carotenoid and anthocyanin of eight pomegranate individuals showed consistent dynamic trends, with no significant difference with the control. Analysis of variance indicated that there were significant differences in the contents of chlorophyll and carotenoid among different pomegranate individuals at different growth stages and in the content of anthocyanin among different pomegranate individuals, while no significant difference was observed in the chlorophyll/carotenoid ratio among different pomegranate individuals at different growth stages. [Conclusion] The differences in leaf colors among pomegranate hybrid progeny are caused by different contents and ratios of anthocyanin and chlorophyll at growth stage.

Effect of Nutrimental Stress on the Pigment Content of the Heliophytic Poinsettia （Euphorbia Pulcherrima cv. Valenciana）

Nutrimental requirements of heliophytic poinsettias have not yet been determined, this information is essential for developing an adequate technological package to orient producers in its management. The behavior of the plant under nutritional stress needs to be known by the application of a hydroponic system. The objective of the present work was to evaluate the physiological effect of induced deficiencies of five macronutriments （nitrogen, phosphorous, potassium, calcium, and magnesium） and its influence on the pigment production of the poinsettia Euphorbia puleherrima cv. ＂Valenciana＂. The study was made during 2010 at the Research Unit ＂Dr. Ramon Fernandez Gonzalez＂ in Texcoco, State of Mexico. Six treatments were tested during 240 days from transplant in a totally randomized experimental design. Chlorophylls contents were determined at the beginning of the experiment. At the end of the treatment period, the contents of nutrients, chlorophylls, leaf carotenoids and bract anthocyanins were determined. The effects observed as results of the treatments were： reduction in plant height and diameter; the lowest content of total chlorophylls was observed in the absence of nitrogen, the largest reduction being of chlorophyll a and carotenoids; a low content of anthocyanins was observed to be associated with low nitrogen and calcium; a high correlation was found between nitrogen supplementation and measured non-destructively with a SPAD 502 Minolta unit values measured intensity of green color of leaves.