不同浓度花浸提液对红三叶种子化感作用研究

以普通红三叶种子为受体,选取新鲜红三叶植株的花,设计了0.025 g/m L(DW)、0.05 g/m L(DW)、0.075 g/m L(DW)、0.10 g/m L(DW)、0.15 g/m L(DW)、0.20 g/m L(DW)6种不同浓度的水浸提液,研究了红三叶的花浸提液对其自身种子发芽势、发芽率、幼根长和下胚轴长的化感影响。结果表明:红三叶的花能够分泌出化感物质,对种子发育具有化感作用。不同浓度的红三叶浸提液对种子的发芽势、发芽率表现出"低促高抑"的现象;不同浓度红三叶花浸提液的化感物质对种子的幼根具有极强的抑制作用(p<0.01),而对下胚轴长既具有抑制作用又有促进作用。

凌霄干花水浸提液对高羊茅种子萌发和幼苗生长的化感作用

实验以5个不同浓度梯度的实验组和对照组为比照对象,研究不同浓度的凌霄干花水浸提液对高羊茅种子萌发、幼苗生长的影响,探究凌霄干花水浸提液对高羊茅种子及幼苗生长化感效应的差异。实验结果表明:凌霄干花水浸提液浓度对受体高羊茅种子发芽的化感效应表现为低浓度(0.01 g/mL)促进其萌发,高浓度(大于等于0.02 g/mL)抑制其幼苗生长,且对高羊茅发芽指数的抑制强度明显强于对发芽率的抑制强度;凌霄花水浸提液对幼苗生长情况的4个指标(苗长、根长、苗鲜重和根鲜重)的化感作用表现为低促高抑,且化感抑制强度依次为苗长<根鲜重<苗鲜重<根长。本实验意在为凌霄干花的化感作用研究提供基本依据,同时也为凌霄的种植布局提供理论支撑。

Effect of Cotton Straw Extract on Wheat Root Growth

Objective] The aim was to study the effects of cotton straw extract on wheat root growth and define the al elopathic effect of cotton straw on wheat root. [Method] A sand culture experiment was conducted under different cotton straw ex-tract levels （0%, 1.0%, 2.0%, 4.0%, 8.0% and 10.0%）, and the morphology and physiological activity of wheat （Yangmai 13） root were studied. [Result] The cotton straw extract inhibited the elongation of wheat roots, but increased the root diame-ter, resulting in the decreased root surface area and volume; The extract treatments induced the increase of root reactive oxygen species （ROS） level, which led to an increased root membrane lipid peroxidation, thereby reducing the root vigor and in-hibiting the root water uptake and formation of biomass; With the increase of con-centration of cotton straw extract, the total phenolic acid content in the extract in-creased continuously, and the total phenolic acid content was closely related to the morphogenesis and physiological activity of wheat root. [Conclusion] The cotton straw extract inhibited the morphogenesis and physiological activity of wheat root through affecting the root ROS level, and the total phenolic content was one of the main limiting factors.