以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲...以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲霉侵染过程中PnLOX2基因表达量变化显著,而金花1012的表达量变化较小,表明PnLOX2基因在花生种皮中存在并且可能与抗黄曲霉相关。展开更多
[Objective] The aim was to explore the production potential and extension value of Yun peanut No.3 to lay foundation for further extension. [Method] Selection, breeding, characters, yield and the stability of Yun pean...[Objective] The aim was to explore the production potential and extension value of Yun peanut No.3 to lay foundation for further extension. [Method] Selection, breeding, characters, yield and the stability of Yun peanut No.3 were analyzed. [Result] Yun peanut No.3 is a new early-mature peanut species bred based on foreign resources and local varieties through sexual hybridization and improvement. It contains 50.32% of fat, 26.5% of protein, 38.93% of oleic acid, 38.88% of linoleic acid, and the ratio of oleic acid and linoleic acid is at 1.00. It is a typical small-scale peanut with red skin, high contents of oil and fat, and constitutes the first one approved by cultivar registration in China. According to related tests, pod yield achieved 3 615.9 kg/hm2 ; kernel yield was 2 570.1 kg/hm2 and oil output was 1 293.3 kg/hm2 . For the production at scale, pod yields would be about 3 000 kg/hm2 ; the production value of fruit would be 25 000 Yuan/hm2 ; the yield per unit area would be maximal at 16 000 kg/hm2 , totaling 64 000 Yuan/hm2 . It enjoys different excellent qualities, such as drought-resistance and barren-resistance. It was approved in Yunnan Province in 2012 and extended at 20 000 Yuan/hm2 in major production lands. [Conclusion] Yun peanut No.3, which is highly-yield and highly-qualified, is suitable to be grown in Yunnan, as well as similar conditions in southern regions in China.展开更多
文摘以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲霉侵染过程中PnLOX2基因表达量变化显著,而金花1012的表达量变化较小,表明PnLOX2基因在花生种皮中存在并且可能与抗黄曲霉相关。
基金Supported by Yunnan Key New Cultivar Planting Program (2011BB010)National Penaut Industry Technology System (CARS-14)~~
文摘[Objective] The aim was to explore the production potential and extension value of Yun peanut No.3 to lay foundation for further extension. [Method] Selection, breeding, characters, yield and the stability of Yun peanut No.3 were analyzed. [Result] Yun peanut No.3 is a new early-mature peanut species bred based on foreign resources and local varieties through sexual hybridization and improvement. It contains 50.32% of fat, 26.5% of protein, 38.93% of oleic acid, 38.88% of linoleic acid, and the ratio of oleic acid and linoleic acid is at 1.00. It is a typical small-scale peanut with red skin, high contents of oil and fat, and constitutes the first one approved by cultivar registration in China. According to related tests, pod yield achieved 3 615.9 kg/hm2 ; kernel yield was 2 570.1 kg/hm2 and oil output was 1 293.3 kg/hm2 . For the production at scale, pod yields would be about 3 000 kg/hm2 ; the production value of fruit would be 25 000 Yuan/hm2 ; the yield per unit area would be maximal at 16 000 kg/hm2 , totaling 64 000 Yuan/hm2 . It enjoys different excellent qualities, such as drought-resistance and barren-resistance. It was approved in Yunnan Province in 2012 and extended at 20 000 Yuan/hm2 in major production lands. [Conclusion] Yun peanut No.3, which is highly-yield and highly-qualified, is suitable to be grown in Yunnan, as well as similar conditions in southern regions in China.
