目的观察黑米花色苷提取物(anthocyanin-rich extract from black rice,AEBR)的抗过敏作用并探讨其可能的作用机制。方法通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验,用比色法测定黑米花色苷提取物在体内对肥大细胞影响;体外实验观察黑...目的观察黑米花色苷提取物(anthocyanin-rich extract from black rice,AEBR)的抗过敏作用并探讨其可能的作用机制。方法通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验,用比色法测定黑米花色苷提取物在体内对肥大细胞影响;体外实验观察黑米花色苷提取物对IgE诱导组胺释放、细胞内钙摄入及其他炎性介子的影响。结果动物实验显示,AEBR高、中剂量组(300、150 mg/kg)明显抑制PCA实验,抑制率分别49.71%、30.40%。细胞实验显示,AEBR高、中剂量组(100、50μg/L)明显抑制组胺的释放(抑制率分别为75.56%、44.76%)、细胞内钙摄入(抑制率分别为64.75%、44.56%)以及肿瘤坏死因子-α(TNF-α)[黑米花色苷提取物(25、50、100μg/L)给药10 min使TNF-α蛋白表达分别下降7.8%、80%与85%]、白细胞介素6(IL-6)[黑米花色苷提取物(25、50、100μg/L)给药10min使IL-6蛋白表达分别下降5.6%,77%与84.1%]的释放(P<0.05)。结论黑米花色苷提取物抗过敏作用与抑制肥大细胞脱颗粒及炎性介子的释放有关。展开更多
This study aimed to explore the main factors influencing the extraction of anthocyanins from strawberry, such as solid-liquid ratio, concentration of solvent, duration of ultrasonic treatment and ultrasonic power. Acc...This study aimed to explore the main factors influencing the extraction of anthocyanins from strawberry, such as solid-liquid ratio, concentration of solvent, duration of ultrasonic treatment and ultrasonic power. According to the results of sin-gle factor test, an orthogonal experiment was designed to determine the optimum extraction conditions. The effects of the four factors on anthocyanins extraction from strawberry were listed here in an decreasing order： ethanol concentration 〉 solid-liq-uid ratio〉duration of ultrasonic treatment〉ultrasonic power. And the optimal condi-tions of ultrasonic extraction of anthocyanins from strawberry were： ultrasonic treat-ment of 30 min, solid-liquid ratio of 1：15, ethanol concentration of 60% and ultra-sonic power of 300 W. Under such conditions, the content of extracted antho-cyanins was 33.247 mg/100g, which was 1.3 folds higher than that by extraction without ultrasonic treatment.展开更多
文摘目的观察黑米花色苷提取物(anthocyanin-rich extract from black rice,AEBR)的抗过敏作用并探讨其可能的作用机制。方法通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验,用比色法测定黑米花色苷提取物在体内对肥大细胞影响;体外实验观察黑米花色苷提取物对IgE诱导组胺释放、细胞内钙摄入及其他炎性介子的影响。结果动物实验显示,AEBR高、中剂量组(300、150 mg/kg)明显抑制PCA实验,抑制率分别49.71%、30.40%。细胞实验显示,AEBR高、中剂量组(100、50μg/L)明显抑制组胺的释放(抑制率分别为75.56%、44.76%)、细胞内钙摄入(抑制率分别为64.75%、44.56%)以及肿瘤坏死因子-α(TNF-α)[黑米花色苷提取物(25、50、100μg/L)给药10 min使TNF-α蛋白表达分别下降7.8%、80%与85%]、白细胞介素6(IL-6)[黑米花色苷提取物(25、50、100μg/L)给药10min使IL-6蛋白表达分别下降5.6%,77%与84.1%]的释放(P<0.05)。结论黑米花色苷提取物抗过敏作用与抑制肥大细胞脱颗粒及炎性介子的释放有关。
基金Supported by National Natural Science Foundation of China(31201402)Research Award Fund for Outstanding Young Scientist of Shandong Province(BS2011NY016)~~
文摘This study aimed to explore the main factors influencing the extraction of anthocyanins from strawberry, such as solid-liquid ratio, concentration of solvent, duration of ultrasonic treatment and ultrasonic power. According to the results of sin-gle factor test, an orthogonal experiment was designed to determine the optimum extraction conditions. The effects of the four factors on anthocyanins extraction from strawberry were listed here in an decreasing order： ethanol concentration 〉 solid-liq-uid ratio〉duration of ultrasonic treatment〉ultrasonic power. And the optimal condi-tions of ultrasonic extraction of anthocyanins from strawberry were： ultrasonic treat-ment of 30 min, solid-liquid ratio of 1：15, ethanol concentration of 60% and ultra-sonic power of 300 W. Under such conditions, the content of extracted antho-cyanins was 33.247 mg/100g, which was 1.3 folds higher than that by extraction without ultrasonic treatment.
