A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expre...A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.展开更多
Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild...Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.展开更多
The Shigujian pluton is a gneissic quartz monzonite located in Tiantangzhai area in central part of the Dabie orogen.Anisotropy of magnetic susceptibility(AMS) data show that most magnetic foliations dip steeply to so...The Shigujian pluton is a gneissic quartz monzonite located in Tiantangzhai area in central part of the Dabie orogen.Anisotropy of magnetic susceptibility(AMS) data show that most magnetic foliations dip steeply to southeast.About 85% of sampling points dip from 40° to 90°.Magnetic foliations are generally parallel to the foliations measured in the field.The pluton has NWW-SEE trending lineations in the southeast and NE-SW trending lineations in central part and north,but the lineations plunge to SW in central part and to NE in the north.All plunges are moderate.The anisotropy degree(P) is between 1.065 and 1.532 and the shape parameter(T) is between 0.005 and 0.694.A Flinn diagram of the magnetic fabrics shows that the value of K is less than 1.The analysis of AMS suggests that the pluton was emplaced and deformed under a SE-NW compressional stress regime.The analysis of quartz C-axis fabrics indicates that the pluton was deformed under compressional stress and deformation temperatures range from 400 to 500℃.Microstructures indicate that the pluton is deformed in near solidus conditions and the pluton is a synkinematic intrusion.The emplacement of the Shigujian granite is inferred to have taken place syntectonically.The zircon U-Pb dating of the granite suggests that the pluton was intruded at 141±2.3 Ma.By synthesizing all data,it seems that the Shigujian pluton was emplaced in a compressional environment and the transformation time of the Dabie orogen from compression to extension took place after 141 Ma.The structural evolution of the Dabie orogen was controlled by the Pacific tectonic domain when the Shigujian pluton was emplaced,whereas the adjacent Tiantangzhai complex massif is the result of an extensional environment.展开更多
文摘A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.
文摘Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.
基金supported by National Natural Science Foundation of China (Grant Nos. 40972137,41172189)
文摘The Shigujian pluton is a gneissic quartz monzonite located in Tiantangzhai area in central part of the Dabie orogen.Anisotropy of magnetic susceptibility(AMS) data show that most magnetic foliations dip steeply to southeast.About 85% of sampling points dip from 40° to 90°.Magnetic foliations are generally parallel to the foliations measured in the field.The pluton has NWW-SEE trending lineations in the southeast and NE-SW trending lineations in central part and north,but the lineations plunge to SW in central part and to NE in the north.All plunges are moderate.The anisotropy degree(P) is between 1.065 and 1.532 and the shape parameter(T) is between 0.005 and 0.694.A Flinn diagram of the magnetic fabrics shows that the value of K is less than 1.The analysis of AMS suggests that the pluton was emplaced and deformed under a SE-NW compressional stress regime.The analysis of quartz C-axis fabrics indicates that the pluton was deformed under compressional stress and deformation temperatures range from 400 to 500℃.Microstructures indicate that the pluton is deformed in near solidus conditions and the pluton is a synkinematic intrusion.The emplacement of the Shigujian granite is inferred to have taken place syntectonically.The zircon U-Pb dating of the granite suggests that the pluton was intruded at 141±2.3 Ma.By synthesizing all data,it seems that the Shigujian pluton was emplaced in a compressional environment and the transformation time of the Dabie orogen from compression to extension took place after 141 Ma.The structural evolution of the Dabie orogen was controlled by the Pacific tectonic domain when the Shigujian pluton was emplaced,whereas the adjacent Tiantangzhai complex massif is the result of an extensional environment.
