A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expre...A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.展开更多
Mg65Cu25Y10 bulk amorphous alloy specimens prepared by conventional copper mould method were heated at 200 °C for different time and the phase contents as well as microstructure were studied.The XRD results show ...Mg65Cu25Y10 bulk amorphous alloy specimens prepared by conventional copper mould method were heated at 200 °C for different time and the phase contents as well as microstructure were studied.The XRD results show that the crystallization of Mg65Cu25Y10 bulk amorphous alloy specimen becomes complete as the treating time increases and Mg2Cu,Mg24Y5 and HCP-Mg crystalline phases are found.Snowflake-like morphology is found in different specimens through SEM observation.The EDS patterns show that the composition of the snowflake-like structure is close to that of the as-cast alloy.Laminated structures are observed from the TEM images of the snowflake-like structure.From the electron diffraction patterns,it is seen that the snowflake-like structure is the combination of Mg24Y5 and amorphous matrix.The FCC-Mg phase in the matrix transforms into HCP-Mg during the heat-treating process.展开更多
With Adaptation Theory as its theoretical basis, this research makes comparison between Lisa See＇s original version of Snow Flower and the Secret Fan and Xin Yuanjie＇s Chinese translation based on a self-compiled ma...With Adaptation Theory as its theoretical basis, this research makes comparison between Lisa See＇s original version of Snow Flower and the Secret Fan and Xin Yuanjie＇s Chinese translation based on a self-compiled manual annotation English-Chinese bilingual corpus. It aims at exploring the choice of language and the choice of translation method in the foreign language creation and rootless back-translation of Snow Flower and the Secret Fan, discussing whether these choices adapt to the source language or the target language, and finding out the communicative effects they have achieved. The preliminary results show that： the choice of language and the choice of translation method in Foreign Language Creation mainly adapt to the target language to make easier for the target English readers to understand and partly adapt to the source language to keep local flavor; the choice of language and the choice of translation method in rootless back translation mainly adapt to the target language to make the translation authentic, accurate and smooth, and adaption to source language will lead to translationese. This research sheds new light on this special kind of writing and translation.展开更多
文摘A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.
基金Project (2008-04) supported by the Top Talent Plan of Jiangsu University, ChinaProject (10KJA430008) supported by the Natural Science Foundation of Jiangsu Higher Education Institutions,China
文摘Mg65Cu25Y10 bulk amorphous alloy specimens prepared by conventional copper mould method were heated at 200 °C for different time and the phase contents as well as microstructure were studied.The XRD results show that the crystallization of Mg65Cu25Y10 bulk amorphous alloy specimen becomes complete as the treating time increases and Mg2Cu,Mg24Y5 and HCP-Mg crystalline phases are found.Snowflake-like morphology is found in different specimens through SEM observation.The EDS patterns show that the composition of the snowflake-like structure is close to that of the as-cast alloy.Laminated structures are observed from the TEM images of the snowflake-like structure.From the electron diffraction patterns,it is seen that the snowflake-like structure is the combination of Mg24Y5 and amorphous matrix.The FCC-Mg phase in the matrix transforms into HCP-Mg during the heat-treating process.
文摘With Adaptation Theory as its theoretical basis, this research makes comparison between Lisa See＇s original version of Snow Flower and the Secret Fan and Xin Yuanjie＇s Chinese translation based on a self-compiled manual annotation English-Chinese bilingual corpus. It aims at exploring the choice of language and the choice of translation method in the foreign language creation and rootless back-translation of Snow Flower and the Secret Fan, discussing whether these choices adapt to the source language or the target language, and finding out the communicative effects they have achieved. The preliminary results show that： the choice of language and the choice of translation method in Foreign Language Creation mainly adapt to the target language to make easier for the target English readers to understand and partly adapt to the source language to keep local flavor; the choice of language and the choice of translation method in rootless back translation mainly adapt to the target language to make the translation authentic, accurate and smooth, and adaption to source language will lead to translationese. This research sheds new light on this special kind of writing and translation.
