The outbreak of oxidative stress, inflammatory diseases and mycoses, constitute an important public health problem. This could be due to the increase of risk factors, side effects and expensive therapeutic molecules a...The outbreak of oxidative stress, inflammatory diseases and mycoses, constitute an important public health problem. This could be due to the increase of risk factors, side effects and expensive therapeutic molecules available. In the aim to find the potential spring of new therapeutic molecules with efficient and wide spectrum of action, the antiradical, anti-inflammatory and antifungal activities of two essential oils were evaluated. The essential oils were extracted by hydrodistillation. The antiradical activity was evaluated to use DPPH （2,2-diphenyl-l-picrylhydrazyl） scavenging method and the anti-inflammatory activity was determined using the enzymatic method. The disc diffusion and dilution methods were used to evaluate growth inhibition of three yeasts, three moulds and three dermatophytes. The yields of extraction of Apium graveolens and Thymus vulgaris essential oils were 0.14% and 0.32% respectively. These essential oils showed antiradical properties with respective SC50 of 0.41 and 0.06 g/L for Apium graveolens and Thymus vulgaris. Only Thymus vulgaris presented an anti-inflammatory activity with an IC50 of 0.19 g/L. Cryptococcus neoformans was the most susceptible fungal strain while C. albicans was the most resistant one. The results were compared with the standard antifungal. These results show that these essential oils could be exploited as potential spring of molecules endowed with antiradical, anti-inflammatory and antifungal activities.展开更多
An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochlor...An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochloric acid （HC1） and methanol to extract and hydrolyze flavone glycosides simultaneously from powdered FC followed by HPLC to determine the contents of three flavone aglycones, luteolin, apigenin and diosmetin, which are the major bioactive flavones in FC. The extraction and hydrolysis conditions optimized by the orthogonal tests were as follows： powdered FC was refluxed in 80% methanol （v/v） containing 2.4 M HC1 for 2 h in 80 ℃ water bath. The HPLC separation was performed on C18 column and detected with DAD at 344 nm. The calibration curves for luteolin, apigenin and diosmetin were linear over the ranges of 2.233-71.46 μg/mL, 1.668-53.38 μg/mL and 1.410-45.11 μg/mL, their assay recoveries were 100.3%, 103.9% and 100.4%, their intra-day repeatability （R.S.D.） were 0.68%, 0.21% and 0.38% and their inter-day repeatability were 2.6%, 1.7% and 2.0%, respectively. The developed method has been successfully utilized to assay eight species of popular FC samples, especially to analyze Hangbaiju, the well-known FC in China.展开更多
文摘The outbreak of oxidative stress, inflammatory diseases and mycoses, constitute an important public health problem. This could be due to the increase of risk factors, side effects and expensive therapeutic molecules available. In the aim to find the potential spring of new therapeutic molecules with efficient and wide spectrum of action, the antiradical, anti-inflammatory and antifungal activities of two essential oils were evaluated. The essential oils were extracted by hydrodistillation. The antiradical activity was evaluated to use DPPH （2,2-diphenyl-l-picrylhydrazyl） scavenging method and the anti-inflammatory activity was determined using the enzymatic method. The disc diffusion and dilution methods were used to evaluate growth inhibition of three yeasts, three moulds and three dermatophytes. The yields of extraction of Apium graveolens and Thymus vulgaris essential oils were 0.14% and 0.32% respectively. These essential oils showed antiradical properties with respective SC50 of 0.41 and 0.06 g/L for Apium graveolens and Thymus vulgaris. Only Thymus vulgaris presented an anti-inflammatory activity with an IC50 of 0.19 g/L. Cryptococcus neoformans was the most susceptible fungal strain while C. albicans was the most resistant one. The results were compared with the standard antifungal. These results show that these essential oils could be exploited as potential spring of molecules endowed with antiradical, anti-inflammatory and antifungal activities.
基金The Ministry of Sciences and Technology of China (Grant No. 2006BAI06A18-11)Administration and Traditional Chinese Medicine,Zhejiang,China (Grant No. 2007ZA012)
文摘An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochloric acid （HC1） and methanol to extract and hydrolyze flavone glycosides simultaneously from powdered FC followed by HPLC to determine the contents of three flavone aglycones, luteolin, apigenin and diosmetin, which are the major bioactive flavones in FC. The extraction and hydrolysis conditions optimized by the orthogonal tests were as follows： powdered FC was refluxed in 80% methanol （v/v） containing 2.4 M HC1 for 2 h in 80 ℃ water bath. The HPLC separation was performed on C18 column and detected with DAD at 344 nm. The calibration curves for luteolin, apigenin and diosmetin were linear over the ranges of 2.233-71.46 μg/mL, 1.668-53.38 μg/mL and 1.410-45.11 μg/mL, their assay recoveries were 100.3%, 103.9% and 100.4%, their intra-day repeatability （R.S.D.） were 0.68%, 0.21% and 0.38% and their inter-day repeatability were 2.6%, 1.7% and 2.0%, respectively. The developed method has been successfully utilized to assay eight species of popular FC samples, especially to analyze Hangbaiju, the well-known FC in China.
