The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. ...The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.展开更多
A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being high...A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase (SOD) activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde (MDA) content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.展开更多
[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, ...[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS+0.2 mg/L 6-BA+0.2 mg/L 2,4-D, MS+I.0 mg/L 6-BA+0.2 mg/L IAA, MS+ 1.0 mg/L KT+0.5 mg/L 2,4-D, respectively.展开更多
A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRN...A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios: Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows: KB culture medium (peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL); temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% (v/v).展开更多
[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and externa...[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and external morphology. [Method] The paraffin section method was used to observe the flower bud of Gentiana triflora un- der microscope. [Result] The flower bud differentiation process of Gentiana triflora could be divided into six stages: the undifferentiated stage, initial differentiation stage, sepal primordia differentiation stage, petal primordia differentiation stage, stamen pri- mordia differentiation stage and the pistil primordia differentiation stage. The flower bud differentiation started from early May, when the plant had 10-11 stem nodes, and completed in early June, lasted about 40 d. The effect of plant height on flower bud differentiation was not significant. [Conclusion] The study provided theoretical basis for the high-yield cultivation, variety selection and flowering regulation of Gentiana triflora.展开更多
With Welsh Onion seeds employed as materials, effects of magnetized water on seed Germination were studied. The results showed the treatment of magnetized water soaking for 4 h promoted water absorption rate and amyla...With Welsh Onion seeds employed as materials, effects of magnetized water on seed Germination were studied. The results showed the treatment of magnetized water soaking for 4 h promoted water absorption rate and amylase ac- tivities of seeds significantly, which accelerated the transformation process of en- dosperm starch to soluble sugar, resulting in emergence of 36 hours in advance under low temperature condition. Germination rate and germination potential of magnetized water soaking were higher than the contrast by 6.7% and 10.0%, which helped cultivate vigorous seedling.展开更多
文摘The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.
文摘A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase (SOD) activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde (MDA) content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.
基金Supported by Special Fund for the Dominant Subjects Development of the Higher Education Institutions of Jiangsu ProvinceScience and Technology Innovation Fund of Nanjing Forestry University(X09-120-4)~~
文摘[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS+0.2 mg/L 6-BA+0.2 mg/L 2,4-D, MS+I.0 mg/L 6-BA+0.2 mg/L IAA, MS+ 1.0 mg/L KT+0.5 mg/L 2,4-D, respectively.
基金supported by the Scientific Research Foundation of Xiamen Marine Research and Development Institute (No. K10102 (1))
文摘A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios: Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows: KB culture medium (peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL); temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% (v/v).
基金Supported by the Youth Fund of Heilongjiang University,China~~
文摘[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and external morphology. [Method] The paraffin section method was used to observe the flower bud of Gentiana triflora un- der microscope. [Result] The flower bud differentiation process of Gentiana triflora could be divided into six stages: the undifferentiated stage, initial differentiation stage, sepal primordia differentiation stage, petal primordia differentiation stage, stamen pri- mordia differentiation stage and the pistil primordia differentiation stage. The flower bud differentiation started from early May, when the plant had 10-11 stem nodes, and completed in early June, lasted about 40 d. The effect of plant height on flower bud differentiation was not significant. [Conclusion] The study provided theoretical basis for the high-yield cultivation, variety selection and flowering regulation of Gentiana triflora.
基金Supported by China Spark Program for Science and Technology(2011GA740072)Shandong Provincial Soft Scientific Research Project(2015RKC35001)Shandong Provincial Agricultural High-quality Seed Engineering(2016LZGC019)~~
文摘With Welsh Onion seeds employed as materials, effects of magnetized water on seed Germination were studied. The results showed the treatment of magnetized water soaking for 4 h promoted water absorption rate and amylase ac- tivities of seeds significantly, which accelerated the transformation process of en- dosperm starch to soluble sugar, resulting in emergence of 36 hours in advance under low temperature condition. Germination rate and germination potential of magnetized water soaking were higher than the contrast by 6.7% and 10.0%, which helped cultivate vigorous seedling.