In a completely randomized blocks （RCB） plot, we planted seed from the three segments of Kponan tubers： head, median and tail portions with control Krengle. Germination began 10 d and lasted 67 d. The mean sproutin...In a completely randomized blocks （RCB） plot, we planted seed from the three segments of Kponan tubers： head, median and tail portions with control Krengle. Germination began 10 d and lasted 67 d. The mean sprouting rate of the Kponan full season harvest tuber was 78.1% compared to 94% in Krengle. However, the tuber germination was progressive from head to tail with a linear evolution of the top fragments with sprouting rate up to 90% similar to Krengle. The median and distal portions sprout two and three weeks later with final raising rates reaching 68% and 71%, respectively. The full season tuber of Kponan sprouts with a good rate gave good yields for any portion of the tuber. It had a sprouting gradient oriented from the proximal portion to the distal one, while yield was better for medium seeds. The sprouting delays two weeks between proximal and medium portions and three weeks between proximal and distal portions were acceptable with regards to the total cycle of eight to nine months period. Thus, its small tubers could be planted as well as the top portion of big tuber. In terms of yield, Kponan medium seed gave the best yield 34.25 t/ha and the whole Kponan tuber yield was more than Krengle. That led to a better multiplication factor for Kponan full season tuber. The type of tuber, if available, could be used as seed for yam production. That could improve its distribution and contribute to food security.展开更多
Abstract： Raphunus sativus L. commonly known as ＂radish＂ is a popular vegetable crop used by people all over the world for its culinary and medicinal properties. Enhancement of in vitro regeneration system for radi...Abstract： Raphunus sativus L. commonly known as ＂radish＂ is a popular vegetable crop used by people all over the world for its culinary and medicinal properties. Enhancement of in vitro regeneration system for radish variety beeralu is needed to further tissue culture studied. Therefore, the present study was carried out to find out the effects of different organic additives on in vitro shoot regeneration of Radish （Raphanus sativus L.） Var. Beeralu. Hypocotyl explants of aseptic plantlets were cultured on MS basal medium supplemented with 2.5 mg/L BAP （benzyl adenine） and 0.1 mg/L NAA （1-nphthaleneacetic acid） with different natural additives; 20% coconut water, 20% coconut milk, 10% grind spinach leaves, 10% grind potato tubers, 10% grind carrot, 5% rice flour, 10% green gram, 10% grind pumpkin, 10% banana fruit, 10% orange and control （without any additives）. Complete randomized design （CRD） with five replicates was used. After one month the numbers of regenerated shoots were counted and statistical analysis was carried out using the Student Newman-Kuells Means Separation Test of SAS program （9.1.3）. The highest mean number of shoots （12 shoots/explant） from Radish （Raphanus sativus L.） Var. Beeralu observed in MS basal medium with 2.5 mg/L BAP and 0.1 mg/L NAA with 10% orange juice whereas the 2nd highest shoots were obtained with 20% coconut water. The lowest number of shoots （0 shoot/explant） was observed from medium with carrot juice and pumpkin juice, but they induced callus formation. Media with grind spinach leaves, rice flour, green gram, grind potato tubers and banana inhibit the shoot regeneration.展开更多
文摘In a completely randomized blocks （RCB） plot, we planted seed from the three segments of Kponan tubers： head, median and tail portions with control Krengle. Germination began 10 d and lasted 67 d. The mean sprouting rate of the Kponan full season harvest tuber was 78.1% compared to 94% in Krengle. However, the tuber germination was progressive from head to tail with a linear evolution of the top fragments with sprouting rate up to 90% similar to Krengle. The median and distal portions sprout two and three weeks later with final raising rates reaching 68% and 71%, respectively. The full season tuber of Kponan sprouts with a good rate gave good yields for any portion of the tuber. It had a sprouting gradient oriented from the proximal portion to the distal one, while yield was better for medium seeds. The sprouting delays two weeks between proximal and medium portions and three weeks between proximal and distal portions were acceptable with regards to the total cycle of eight to nine months period. Thus, its small tubers could be planted as well as the top portion of big tuber. In terms of yield, Kponan medium seed gave the best yield 34.25 t/ha and the whole Kponan tuber yield was more than Krengle. That led to a better multiplication factor for Kponan full season tuber. The type of tuber, if available, could be used as seed for yam production. That could improve its distribution and contribute to food security.
文摘Abstract： Raphunus sativus L. commonly known as ＂radish＂ is a popular vegetable crop used by people all over the world for its culinary and medicinal properties. Enhancement of in vitro regeneration system for radish variety beeralu is needed to further tissue culture studied. Therefore, the present study was carried out to find out the effects of different organic additives on in vitro shoot regeneration of Radish （Raphanus sativus L.） Var. Beeralu. Hypocotyl explants of aseptic plantlets were cultured on MS basal medium supplemented with 2.5 mg/L BAP （benzyl adenine） and 0.1 mg/L NAA （1-nphthaleneacetic acid） with different natural additives; 20% coconut water, 20% coconut milk, 10% grind spinach leaves, 10% grind potato tubers, 10% grind carrot, 5% rice flour, 10% green gram, 10% grind pumpkin, 10% banana fruit, 10% orange and control （without any additives）. Complete randomized design （CRD） with five replicates was used. After one month the numbers of regenerated shoots were counted and statistical analysis was carried out using the Student Newman-Kuells Means Separation Test of SAS program （9.1.3）. The highest mean number of shoots （12 shoots/explant） from Radish （Raphanus sativus L.） Var. Beeralu observed in MS basal medium with 2.5 mg/L BAP and 0.1 mg/L NAA with 10% orange juice whereas the 2nd highest shoots were obtained with 20% coconut water. The lowest number of shoots （0 shoot/explant） was observed from medium with carrot juice and pumpkin juice, but they induced callus formation. Media with grind spinach leaves, rice flour, green gram, grind potato tubers and banana inhibit the shoot regeneration.
