A new C-glycosylflavone, named panzhihuacycaside (1), was isolated from the leaves of Cycas panzhihuaensis L. Zhou et S. Y. Yang (Cycadaceae) along with 2,3-dihydrohinokiflavone (2), 5,5',7,7', 4',4'&#...A new C-glycosylflavone, named panzhihuacycaside (1), was isolated from the leaves of Cycas panzhihuaensis L. Zhou et S. Y. Yang (Cycadaceae) along with 2,3-dihydrohinokiflavone (2), 5,5',7,7', 4',4''-hexahydroxy-(2',8')-biflavone (3), vanillic acid (4), P-sitosterol (5) and daucosterol (6). Their structures were elucidated by spectral and chemical evidence.展开更多
[Objective] This study was conducted to isolate and screen new Bacillus thuringiensis （Bt） strains against Lepidoptera insecticides. [Method] Bt strains were isolated from soil of Changbai Mountain area by temperatu...[Objective] This study was conducted to isolate and screen new Bacillus thuringiensis （Bt） strains against Lepidoptera insecticides. [Method] Bt strains were isolated from soil of Changbai Mountain area by temperature screening method, and highly-toxic Bt stains were then selected by biological determination and toxicity de- termination. [Result] From 150 soil samples, 18 Bt isolates were isolated, with an average isolation rate of 12.0%. Specifically, the isolation rate from mountain field was 8.5 %, and the isolation rate from farmland was 16.2%. The results of activity determination showed that there were17, 5 and 4 strains showing lethality rate over 90% against Plutel/a xylosrel/a, Spodoptera litura and Spodptera exigua, respectively, and among them, strain YNI-1 exhibited high activity against all the 3 kinds of in- sects. The results of toxicity determination showed that strain YNl-lhad the best fast-acting property against S.litura and S. exigua; strains with high toxicity against S. exigua were YN6-2〉YN1-1〉YN4-2〉YN2-6 sequentially; and toxicity of strains against S. /itura was in order of YN4-4〉YN1-1 〉YN6-1 〉YN4-1 〉YN2-1. [Conclusion] According to activity determination and toxicity determination, strain YN1-1 was screened as the target strain with wide spectrum, fast-acting property and high toxi- city.展开更多
The phosphatidylinositol 3-kinase(PI3K)-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathwa...The phosphatidylinositol 3-kinase(PI3K)-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study, we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain, a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found, using Real-Time Quantitative PCR(Q-PCR) analysis, that lvakt was upregulated during early white spot syndrome virus(WSSV) infection. Moreover, the PI3K-specific inhibitor, LY294002, reduced viral gene transcription, implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.展开更多
[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected laye...[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected layers. And ILTV TK gene was amplified from these specimens by PCR for initial diagnosis. Virus fluid was isolated and inoculated into SPF chicken embryos via allantoic cavity and chorioallantoic membrane （CAM）, respectively. Hyaluronic acid in allantoic fluid was detected, and CAM lesions were observed. The definite diagnosis was performed through animal regression test. [Result] A 1.3 kbp fragment was amplified from larynx and its secretion of the ILT-suspected chickens. And its amino acid sequence had 98.5% homology to that of ILTV TKgene published in GenBank. After the chicken embryos were inoculated with the isolated ILFV fluid, pox spots, giant polynuclear syncytial cells having intranuclear inclusion bodies were observed in CAM. After being challenged by the IL TV fluid, the chickens showed typical respiratory symptoms and pathological changes of ILT. [Coudusion] A field strain named ILTV XZ09 was isolated from larynx and its secretion of ILT-suspected chickens.展开更多
The Cross River watershed was disturbed by historic logging activity during the past century, but under the management of the USDA （United States Department of Agriculture） Forest Service, the area has mostly recove...The Cross River watershed was disturbed by historic logging activity during the past century, but under the management of the USDA （United States Department of Agriculture） Forest Service, the area has mostly recovered from ecological disturbance. Today a new threat is being imposed by climate change; changes affect not only the temperature but also more extreme wind and rain In 2012, a mega-storm event passed through the north shore region of Lake Superior overwhelming many watersheds with excessive rain and runoff. As part of a Cross River study for the Forest Service, pre- and post-event hydrologic adjustment of the Cross River watershed were captured. Samples were collected for δD and δ18O during April, July, and September to estimate HRT （hydraulic residence time） using the stable isotopes of hydrogen and oxygen, The results showed that water collected throughout the watershed shifted toward the signature of the mega-event precipitation signature, then slowly diffused with new precipitation and fractionation processes that resumed into the summer and fall.展开更多
In the process of transition from one social order to another, immigrants implement intentional or circumstantial adjustment strategies to position themselves in the new social structure, Within the broad perspective ...In the process of transition from one social order to another, immigrants implement intentional or circumstantial adjustment strategies to position themselves in the new social structure, Within the broad perspective of immigrants＇ incorporation into the receiving society, the present paper focuses on analysis at the micro-level, i,e., bottom-up strategies. These coping strategies define the social positioning of an individual. Through the structure-agency theoretical framework, this article analyses adjustment strategies used by immigrants in order to become members of the receiving society. This is discovered through semi-structured biographical interviews with female immigrants to Germany from former Soviet countries conducted by the author in 2012-2013 in Kaiserslautern. Several positioning strategies emerged in the course of these interviews： ＂destination as a way to a better life＂; ＂trip to joint self＂ which includes ＂identity as a task＂ and change of the behavioral patterns; shift in gender roles; usage of the imported educational capital; work of a ＂stranger next door＂; and social networking, The analysis of immigrants＇ adjustment strategies allows one to see the complexity of the incorporation processes into the receiving society： from essential personality traits such as imagination and aspirations, identity and roles to practically applicable aspects such as education, work, and networking.展开更多
文摘A new C-glycosylflavone, named panzhihuacycaside (1), was isolated from the leaves of Cycas panzhihuaensis L. Zhou et S. Y. Yang (Cycadaceae) along with 2,3-dihydrohinokiflavone (2), 5,5',7,7', 4',4''-hexahydroxy-(2',8')-biflavone (3), vanillic acid (4), P-sitosterol (5) and daucosterol (6). Their structures were elucidated by spectral and chemical evidence.
基金Supported by Natural Science Foundation of Yanbian University(2011-34)~~
文摘[Objective] This study was conducted to isolate and screen new Bacillus thuringiensis （Bt） strains against Lepidoptera insecticides. [Method] Bt strains were isolated from soil of Changbai Mountain area by temperature screening method, and highly-toxic Bt stains were then selected by biological determination and toxicity de- termination. [Result] From 150 soil samples, 18 Bt isolates were isolated, with an average isolation rate of 12.0%. Specifically, the isolation rate from mountain field was 8.5 %, and the isolation rate from farmland was 16.2%. The results of activity determination showed that there were17, 5 and 4 strains showing lethality rate over 90% against Plutel/a xylosrel/a, Spodoptera litura and Spodptera exigua, respectively, and among them, strain YNI-1 exhibited high activity against all the 3 kinds of in- sects. The results of toxicity determination showed that strain YNl-lhad the best fast-acting property against S.litura and S. exigua; strains with high toxicity against S. exigua were YN6-2〉YN1-1〉YN4-2〉YN2-6 sequentially; and toxicity of strains against S. /itura was in order of YN4-4〉YN1-1 〉YN6-1 〉YN4-1 〉YN2-1. [Conclusion] According to activity determination and toxicity determination, strain YN1-1 was screened as the target strain with wide spectrum, fast-acting property and high toxi- city.
基金Supported by the Scientific Research Foundation of Third Institute of Oceanography,SOA(No.2011018)the Natural Science Foundation of Fujian Province of China(No.2011J05079)+3 种基金the National Natural Science Foundation of China(No.31001125)the Major State Basic Research Development Program of China(973 Program)(No.2012CB114403)the National High Technology Research and Development Program of China(863 Program)(No.2012AA092205)the China Agriculture Research System-47
文摘The phosphatidylinositol 3-kinase(PI3K)-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study, we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain, a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found, using Real-Time Quantitative PCR(Q-PCR) analysis, that lvakt was upregulated during early white spot syndrome virus(WSSV) infection. Moreover, the PI3K-specific inhibitor, LY294002, reduced viral gene transcription, implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.
文摘[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected layers. And ILTV TK gene was amplified from these specimens by PCR for initial diagnosis. Virus fluid was isolated and inoculated into SPF chicken embryos via allantoic cavity and chorioallantoic membrane （CAM）, respectively. Hyaluronic acid in allantoic fluid was detected, and CAM lesions were observed. The definite diagnosis was performed through animal regression test. [Result] A 1.3 kbp fragment was amplified from larynx and its secretion of the ILT-suspected chickens. And its amino acid sequence had 98.5% homology to that of ILTV TKgene published in GenBank. After the chicken embryos were inoculated with the isolated ILFV fluid, pox spots, giant polynuclear syncytial cells having intranuclear inclusion bodies were observed in CAM. After being challenged by the IL TV fluid, the chickens showed typical respiratory symptoms and pathological changes of ILT. [Coudusion] A field strain named ILTV XZ09 was isolated from larynx and its secretion of ILT-suspected chickens.
文摘The Cross River watershed was disturbed by historic logging activity during the past century, but under the management of the USDA （United States Department of Agriculture） Forest Service, the area has mostly recovered from ecological disturbance. Today a new threat is being imposed by climate change; changes affect not only the temperature but also more extreme wind and rain In 2012, a mega-storm event passed through the north shore region of Lake Superior overwhelming many watersheds with excessive rain and runoff. As part of a Cross River study for the Forest Service, pre- and post-event hydrologic adjustment of the Cross River watershed were captured. Samples were collected for δD and δ18O during April, July, and September to estimate HRT （hydraulic residence time） using the stable isotopes of hydrogen and oxygen, The results showed that water collected throughout the watershed shifted toward the signature of the mega-event precipitation signature, then slowly diffused with new precipitation and fractionation processes that resumed into the summer and fall.
文摘In the process of transition from one social order to another, immigrants implement intentional or circumstantial adjustment strategies to position themselves in the new social structure, Within the broad perspective of immigrants＇ incorporation into the receiving society, the present paper focuses on analysis at the micro-level, i,e., bottom-up strategies. These coping strategies define the social positioning of an individual. Through the structure-agency theoretical framework, this article analyses adjustment strategies used by immigrants in order to become members of the receiving society. This is discovered through semi-structured biographical interviews with female immigrants to Germany from former Soviet countries conducted by the author in 2012-2013 in Kaiserslautern. Several positioning strategies emerged in the course of these interviews： ＂destination as a way to a better life＂; ＂trip to joint self＂ which includes ＂identity as a task＂ and change of the behavioral patterns; shift in gender roles; usage of the imported educational capital; work of a ＂stranger next door＂; and social networking, The analysis of immigrants＇ adjustment strategies allows one to see the complexity of the incorporation processes into the receiving society： from essential personality traits such as imagination and aspirations, identity and roles to practically applicable aspects such as education, work, and networking.
