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琉球病毒、索尔韦齐病毒、苏里斯病毒等三种沙粒病毒实时荧光RT-PCR检测方法研究 被引量:1
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作者 杜珊珊 赖丽金 +7 位作者 李阿茜 黄晓霞 刘洋 王芹 李川 梁米芳 李德新 李建东 《病毒学报》 CAS CSCD 北大核心 2021年第4期809-815,共7页
建立可检测琉球病毒(Ryukyu virus,RYKV),索尔韦齐病毒(Solwezi virus,SOLV)以及苏里斯病毒(Souris virus,SOUV)等三种沙粒病毒的实时荧光定量RT-PCR检测方法。分析三种病毒流行病学分布特征,从国际公共数据库搜索下载基因组序列,进行... 建立可检测琉球病毒(Ryukyu virus,RYKV),索尔韦齐病毒(Solwezi virus,SOLV)以及苏里斯病毒(Souris virus,SOUV)等三种沙粒病毒的实时荧光定量RT-PCR检测方法。分析三种病毒流行病学分布特征,从国际公共数据库搜索下载基因组序列,进行比对分析,确定检测靶标,借助primer premier 6.0生物信息学软件,设计引物和探针,建立实时荧光定量RT-PCR检测方法,利用化学合成和体外转录方法制备模拟样本,比较评价三种方法的检测限、特异性、重复性特征。所建实时荧光定量RT-PCR检测方法均可有效扩增检测病毒RNA靶标,检测限分别为40拷贝/μL、7拷贝/μL和15拷贝/μL,检测汉城病毒、汉滩病毒、登革病毒Ⅰ~Ⅳ型分离株、发热伴血小板减少综合病毒及30份健康人血清样本无非特异性扩增,三种病毒相互间以及其他8种出血热相关沙粒病毒RNA间无交叉反应,重复性比较分析显示变异系数均在2%以内。本研究建立的检测RYKV、SOLV和SOUV三种沙粒病毒的实时荧光RT-PCR方法具备用于相关疑似感染者临床样本、宿主动物标本以及进出口物品的筛查检测的潜力,但因未经基于实际病毒感染样本的比较评价,检测结果的解释仍具有一定的局限性。 展开更多
关键词 沙粒病毒 琉球病毒 索尔韦齐病毒 苏里斯病毒 实时荧光RT-PCR
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税收影响企业债务的理论和实证研究 被引量:1
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作者 陈洪波 《上海财税》 北大核心 2003年第1期32-34,共3页
引言:税收对负债的影响 债务融资和内源融资、外部股权融资一起构成了企业融资的三种方式。企业债务融资最基本的动机是负债比权益成本更低,负债能够提高企业权益资本的收益率,也就是能提高股东的收益。税收对负债比例最大的影响在于企... 引言:税收对负债的影响 债务融资和内源融资、外部股权融资一起构成了企业融资的三种方式。企业债务融资最基本的动机是负债比权益成本更低,负债能够提高企业权益资本的收益率,也就是能提高股东的收益。税收对负债比例最大的影响在于企业所得税提供的税盾作用(taxshield)。按照税法的规定。 展开更多
关键词 苏里斯 吉罗 投资者 企业所得税税率 个人所得税 所得税率 税收减免 税收优惠 实证研究
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被全球人民盯着的礼物
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作者 梨韵 《今日民航》 2010年第12期18-18,共1页
政坛上的风云人物们收到的礼物,总会被全球人民所瞩目,所以这样的礼物并不是那么好拿的。10月28日,为树立财政紧缩状况下政府与民众共患难的形象,英国政府公布了首相卡梅伦和几位大臣最近接收的礼品清单。卡梅伦自担任首相以来,陆续收... 政坛上的风云人物们收到的礼物,总会被全球人民所瞩目,所以这样的礼物并不是那么好拿的。10月28日,为树立财政紧缩状况下政府与民众共患难的形象,英国政府公布了首相卡梅伦和几位大臣最近接收的礼品清单。卡梅伦自担任首相以来,陆续收到了许多礼物,如法国总统尼古拉·萨科齐赠送的红酒、网球拍和钢笔,巴林国王送的珠宝, 展开更多
关键词 卡梅伦 全球人民 贝卢斯科尼 邮包炸弹 苏里斯 爆炸物
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Detailed sedimentary facies of a sandstone reservoir in the eastern zone of the Sulige gas field, Ordos Basin 被引量:10
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作者 LI Yijun ZHAO Yong +2 位作者 YANG Renchao FAN Aiping LI Fuping 《Mining Science and Technology》 EI CAS 2010年第6期891-897,903,共8页
Given sustaining exploration, the eastern zone of the Sulige gas field may soon become a key area of exploitation. In order to explore its genesis, types and distribution of the reservoir sandstones in the eastern zon... Given sustaining exploration, the eastern zone of the Sulige gas field may soon become a key area of exploitation. In order to explore its genesis, types and distribution of the reservoir sandstones in the eastern zone of this gas field, we focused in our study on the provenance and detailed sedimentary facies of sandstone of the He8 (the eighth part of the Shihezi formation, Permian system) and Shanxil (the first part of the Shanxi formation, Permian system) members, based on core observations, analyses in petrography, granularity and logging. The results show that: 1) the sandstone provenance of Shanxil and He8 in the eastern zone of the Sulige gas field is from the north of the Ordos Basin, characterized by dual directions from the north and northeast. 2) The He8 and Shanxil members were deposited in a fluvial-delta sedimentary system. The He8 was mainly deposited in braided rivers, in- cluding braided channels, channel bars, levee and floodplain sub-environments, whereas the Shanxil Member was deposited in braided rivers and deltas, including braided channels, channel bars, floodplains, tributaries and inter-tributary sub-environments. 3) Sedimentary facies bands migrated in drastic fashion towards the basin from the Shanxil to the He8 Member. Base levels of sedi- mentation generally present a trend of small increases in-amplitude, large decreasing amplitudes and slow and gradual Increases. 4) The continuity of the reservoir sandbodies along the source direction is better than that perpendicular to the direction. Compared with Shanxil, both dimensions and continuity of the sandbodies in He8 are better from which we conclude that it is the most fa- vorable part of the reservoir. 展开更多
关键词 Ordos Basin Sulige gas field PERMIAN sandstone reservoir detailed sedimentary facies
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Diagenesis and porosity evolution of sandstone reservoirs in the East II part of Sulige gas field, Ordos Basin 被引量:21
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作者 Yang Renchao Fan Aiping Han Zuozhen Wang Xiuping 《International Journal of Mining Science and Technology》 SCIE EI 2012年第3期311-316,共6页
It is becoming an important controlling factor of gas exploration and exploitation in the east part of Sulige gas field in the Ordos Basin where the reservoir of main gas formations is tight sandstones. Employing expe... It is becoming an important controlling factor of gas exploration and exploitation in the east part of Sulige gas field in the Ordos Basin where the reservoir of main gas formations is tight sandstones. Employing experimental methods of slice identification, casting slice, scan electron microscope, and X-ray diffrac- tions, we studied the characteristics of petrology and diagenesis on reservoirs in Shanl section of Shanxi formation and He8 section of Shihezi formation of the Permian system in the East II part of Sulige gas field. The results include: (1) the main sandstones in these areas are dominated by lithic sandstone and litbic silicarenite with low grade of maturity; (2) the diagenesis of sandstone in these areas mainly include compaction, cementation, corrosion and alteration. Conclusions are as follows: (1) the diagenetic stage reached period B of the middle diagenetic stage; (2) the early diagenetic compaction is one of the main factors to decreasing porosity; (3) the secondary pores formed by corrosion in acidity medium con- ditions in period A of the middle diagenetic stage can distinctly ameliorate the poor reservoir capability of sandstone and, (4) cementation in period B of the middle diagenetic stage is the most important factor leading; to poor physical property of sandstone reservoirs. 展开更多
关键词 Ordos BasinPermianSandstone reservoirsDiagenesisPorosity evolution
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The Maghrebian Scholarship and the Development of Muslim Intelligentsias in Sub-Saharan Africa: A Case Study of the Central Bilad al-Sudan
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作者 Mukhtar Umar Bunza 《History Research》 2013年第5期342-352,共11页
Central Bilad al-Sudan refers to the areas of the ancient Hausa states, and the domains of the ancient Kanem Bornu. As a result of the aged long commercial, cultural, and religious linkages between North Africa and th... Central Bilad al-Sudan refers to the areas of the ancient Hausa states, and the domains of the ancient Kanem Bornu. As a result of the aged long commercial, cultural, and religious linkages between North Africa and the Central Sudan, one finds so many things in common among the two regions in dress, diets, cultural traits, as well as educational and religious practices. The extent of influence of the North African region on the individual and communal lives of the central Sudan has been extraordinarily great. The central Sudan is one of the most Islamized areas of West Africa, and Arabic language is second language to the Muslim communities in domestic and public spheres. And as a result of the entrenchment of Islam among the people due to the activities of North African scholars and traders the area became the hub of Islamic scholarship. The intellectual history of the Central Bilad al-Sudan cannot be complete without the mention of an Algerian scholar, Muhammad ibn Abd-A1-Kareem al-Maghili who in addition to his scholarly influence took appointment as Special Adviser in Kano in the 1460s. The same could be said of an Egyptian prolific writer, Jalal al-Deen al-Suyuti, who settled in Katsina and Gobir and also took the appointment of Grand Qadi in Katsina in the 15th century. Similarly, books of Sanhaja scholars, Shinqit Ulama'a, as well as Spanish-Andalusian scholars via Morocco were made available and widely studied in this part of Muslim world. The combination of the activities of such scholars, literature, and learning tradition were responsible lbr the growth and development of Muslim intelligentsia in the central Bilad al-Sudan since the earliest time, and the influence continues to date. Thus this paper aims at examining the phenomenon, primarily by assessing the influence of scholars, literature, and educational institutions from the Maghreb on the intellectual development and the emergence of native scholars and intelligentsia and on the general scholarly trend in the central Bilad al-Sudan. 展开更多
关键词 the Maghreb scholarship central Sudan Northern Nigeria indigenous intelligentsia
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