Aims:Through the literature review of Miao medicine for liver cancer,this paper discussed the law of Miao medicine for liver cancer.Methods:Using"Zhonghua Bencao Miao medicine"as the source of literature,the...Aims:Through the literature review of Miao medicine for liver cancer,this paper discussed the law of Miao medicine for liver cancer.Methods:Using"Zhonghua Bencao Miao medicine"as the source of literature,the search term was prepared to search the related literature of CNKI database and Wanfang database for the treatment of liver cancer in the Miao medicine from May 2018.The qualified drugs were used to conduct statistical analysis on their family genus,medicinal property,medicinal taste,regression and efficacy.Results:A total of 132 flavors of Miao medicine for liver cancer were included,and the high-frequency family was compositae,liliaceae,and rosaceae,accounting for 22.8%.The medicine was mainly cold medicine,accounting for 75.8%.The main taste of medicine was bitter,hot and sweet,accounting for 75.5%.The main parts of longitude are hot longitude and cold longitude,accounting for 90.5%.Drugs are mainly used to cure poison,tonifying body and vent the blood,a total of 80.4%.Conclusions:Miao medical treatment of liver cancer is based on the dialectic of cold and heat,with proper use of tonifying body and vent the blood drugs.展开更多
CpG oligodeoxynucleotides （CpG ODN） as adjuvant have been extensively studied in recent years. Phosphodiester CpG ODN （PO CpG ODN） can perfectly mimic bacterial DNA in enhancing immune response but are vulnerable ...CpG oligodeoxynucleotides （CpG ODN） as adjuvant have been extensively studied in recent years. Phosphodiester CpG ODN （PO CpG ODN） can perfectly mimic bacterial DNA in enhancing immune response but are vulnerable to nucleases in vivo. This study aimed to evaluate the immunostimulatory potential arid safety of phosphodiester CpG ODN encapsulated in nonphospholipid liposomes. BALB/c mice were immunized intramuscularly with different formulations of liposomes, CpG ODN and hepatitis B surface antigen （HBsAg）. The results demonstrated that the encapsulated PO CpG ODN were protected against rapid degradation in vivo and retained their adjuvant activity. PO CpG ODN encapsulated with HBsAg in liposomes induced strong Th1-biased or Th1/Th2 mixed humoral immune response in mice with the magnitude similar to their phosphothioate equivalent in the same formulation. High IFN-gamma production induced by this formulation confirmed the generation of strong cellular immune response. Additionally, co-delivery of HBsAg arid PO CpG ODN improved the immune response over that obtained with separate delivery. Safety experiment showed that liposome-encapsulaed PO CpG ODN and HBsAg caused mild systemic and moderate local adverse reaction. In conclusion, our data shows that PO CpG ODN encapsulated in liposomes fully exhibit their Th1-type adjuvant activity arid act as a potential adjuvant for vaccines.展开更多
文摘Aims:Through the literature review of Miao medicine for liver cancer,this paper discussed the law of Miao medicine for liver cancer.Methods:Using"Zhonghua Bencao Miao medicine"as the source of literature,the search term was prepared to search the related literature of CNKI database and Wanfang database for the treatment of liver cancer in the Miao medicine from May 2018.The qualified drugs were used to conduct statistical analysis on their family genus,medicinal property,medicinal taste,regression and efficacy.Results:A total of 132 flavors of Miao medicine for liver cancer were included,and the high-frequency family was compositae,liliaceae,and rosaceae,accounting for 22.8%.The medicine was mainly cold medicine,accounting for 75.8%.The main taste of medicine was bitter,hot and sweet,accounting for 75.5%.The main parts of longitude are hot longitude and cold longitude,accounting for 90.5%.Drugs are mainly used to cure poison,tonifying body and vent the blood,a total of 80.4%.Conclusions:Miao medical treatment of liver cancer is based on the dialectic of cold and heat,with proper use of tonifying body and vent the blood drugs.
文摘CpG oligodeoxynucleotides （CpG ODN） as adjuvant have been extensively studied in recent years. Phosphodiester CpG ODN （PO CpG ODN） can perfectly mimic bacterial DNA in enhancing immune response but are vulnerable to nucleases in vivo. This study aimed to evaluate the immunostimulatory potential arid safety of phosphodiester CpG ODN encapsulated in nonphospholipid liposomes. BALB/c mice were immunized intramuscularly with different formulations of liposomes, CpG ODN and hepatitis B surface antigen （HBsAg）. The results demonstrated that the encapsulated PO CpG ODN were protected against rapid degradation in vivo and retained their adjuvant activity. PO CpG ODN encapsulated with HBsAg in liposomes induced strong Th1-biased or Th1/Th2 mixed humoral immune response in mice with the magnitude similar to their phosphothioate equivalent in the same formulation. High IFN-gamma production induced by this formulation confirmed the generation of strong cellular immune response. Additionally, co-delivery of HBsAg arid PO CpG ODN improved the immune response over that obtained with separate delivery. Safety experiment showed that liposome-encapsulaed PO CpG ODN and HBsAg caused mild systemic and moderate local adverse reaction. In conclusion, our data shows that PO CpG ODN encapsulated in liposomes fully exhibit their Th1-type adjuvant activity arid act as a potential adjuvant for vaccines.
