口蹄疫（Foot and mouth disease，FMD）是由FMD病毒（FMDV）引起的偶蹄动物的烈性传染病，患病动物的口、舌、唇、蹄、乳房等部位发生水泡，水泡破溃后形成烂斑。由于该病传染性强，造成的经济损失严重，世界动物卫生组织（OIE）将该...口蹄疫（Foot and mouth disease，FMD）是由FMD病毒（FMDV）引起的偶蹄动物的烈性传染病，患病动物的口、舌、唇、蹄、乳房等部位发生水泡，水泡破溃后形成烂斑。由于该病传染性强，造成的经济损失严重，世界动物卫生组织（OIE）将该病列为实时通报的传染病之一。展开更多
Tomato seedlings damping-off is a limiting factor in commercial greenhouse production. To determine the causal agents of disease, sampling and fungal isolation were performed during 2012. Samples were collected from i...Tomato seedlings damping-off is a limiting factor in commercial greenhouse production. To determine the causal agents of disease, sampling and fungal isolation were performed during 2012. Samples were collected from infected seedlings growing in greenhouses in the Syrian coastal region. Isolation of fungi was done in the laboratories of the Agronomical Reaserch Center, in Lattakia and the molecular analyses were done in the Biotechnology Center at Tishreen University, Lattakia, Syria, during the years 2012, 2013. Eight isolates ofPythium sp. obtained were purified using hyphal tip method （named P1, P2, P3, P4, P5, P6, P7 and P8）. Isolates were morphologically identified by optical microscope, then molecularly Characterized using genus specific ITS primers. The results of morphological characterization of pathogenic species suggested the detection of Pythium aphanidermatum, P. ultimum. The analysis of DNAs from the different isolates with ITS primers, recognizing the inter transcript spacer of nuclear ribosomal DNA proved that the eight, isolates were belonging to the species P. ultimum. The complete sequences of ribosomal DNA internal transcribed spacers regions of selected isolates were determined and submitted to GenBank. The GenBank-BLAST homology search revealed P. ultimum as the most similar sequence （〉 96% identity） with GenBank entry AB355596.展开更多
The mining of placer iron ore greatly influences the surrounding desert grassland.In Agdala Town,Qinghe County,Xinjiang,the soil seed bank is severely damaged,and the utilization and productivity of desert grasslands ...The mining of placer iron ore greatly influences the surrounding desert grassland.In Agdala Town,Qinghe County,Xinjiang,the soil seed bank is severely damaged,and the utilization and productivity of desert grasslands are almost zero,which seriously affects the safety of the ecological environment and the development of local animal husbandry.It is very important to supplement soil seed banks to enhance the species composition of desert grasslands after ecological restoration.In this study,the effects of the seed bank,species composition,and seed burial depth on the seedling emergence rate at placer iron ore site before and after artificial seed bank replenishment were compared using the germination tray method.The original soil seed bank in the study area contained only four species,which were all annual plants.The dominant species were Salsola ruthenica and Corispermum orientale,and the soil seed bank density was very small.The emergence regularity of the soil seed bank was closely related to water content,and the emergence of annual vegetation was explosive.Seed burial depth affected the emergence rate of perennial grass,and we determined that a burial depth of 0–5 cm was the most effective for emergence.This indicates that seedling emergence of artificially supplemented soil seed bank can be completed within 7 days if the sowing depth is appropriate;sowing depths greater than 5 cm affect seed germination.These findings provide a basis for selecting species to populate large areas.展开更多
文摘Tomato seedlings damping-off is a limiting factor in commercial greenhouse production. To determine the causal agents of disease, sampling and fungal isolation were performed during 2012. Samples were collected from infected seedlings growing in greenhouses in the Syrian coastal region. Isolation of fungi was done in the laboratories of the Agronomical Reaserch Center, in Lattakia and the molecular analyses were done in the Biotechnology Center at Tishreen University, Lattakia, Syria, during the years 2012, 2013. Eight isolates ofPythium sp. obtained were purified using hyphal tip method （named P1, P2, P3, P4, P5, P6, P7 and P8）. Isolates were morphologically identified by optical microscope, then molecularly Characterized using genus specific ITS primers. The results of morphological characterization of pathogenic species suggested the detection of Pythium aphanidermatum, P. ultimum. The analysis of DNAs from the different isolates with ITS primers, recognizing the inter transcript spacer of nuclear ribosomal DNA proved that the eight, isolates were belonging to the species P. ultimum. The complete sequences of ribosomal DNA internal transcribed spacers regions of selected isolates were determined and submitted to GenBank. The GenBank-BLAST homology search revealed P. ultimum as the most similar sequence （〉 96% identity） with GenBank entry AB355596.
基金The Project of Xinjiang Uygur Autonomous Region(2020D01A38)。
文摘The mining of placer iron ore greatly influences the surrounding desert grassland.In Agdala Town,Qinghe County,Xinjiang,the soil seed bank is severely damaged,and the utilization and productivity of desert grasslands are almost zero,which seriously affects the safety of the ecological environment and the development of local animal husbandry.It is very important to supplement soil seed banks to enhance the species composition of desert grasslands after ecological restoration.In this study,the effects of the seed bank,species composition,and seed burial depth on the seedling emergence rate at placer iron ore site before and after artificial seed bank replenishment were compared using the germination tray method.The original soil seed bank in the study area contained only four species,which were all annual plants.The dominant species were Salsola ruthenica and Corispermum orientale,and the soil seed bank density was very small.The emergence regularity of the soil seed bank was closely related to water content,and the emergence of annual vegetation was explosive.Seed burial depth affected the emergence rate of perennial grass,and we determined that a burial depth of 0–5 cm was the most effective for emergence.This indicates that seedling emergence of artificially supplemented soil seed bank can be completed within 7 days if the sowing depth is appropriate;sowing depths greater than 5 cm affect seed germination.These findings provide a basis for selecting species to populate large areas.
