This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, incre...This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, increasing income of foresters and protecting ecological environment. Three types of fores{ land, i.e., the evergreen forest land （China fir forest）, deciduous forest land （walnut forest） and shrub land （honey suckle forest.） were selected, and seeds and cut rhizomes of Paris polyphylla Smith produced in Bamian Mountain, Hunan, were used in the study on understory seedling raising and cultivation techniques. The results showed that： when using different propagation materials, seed propagation could be adopted to reduce the consumption of wild resources and provide a large number of seedlings, and was beneficial to large-scale planting; and when conducing seedling and plantlet raising followed by transplanting and direct seeding and planting using different propagation materials in large-scale understory cultivation, direct seeding/ planting was appropriate for saving save production cost with the advantage of early production. Among the 3 different types of forest land, the best choice was broad- leaved forest land, which could achieve better economic benefit because of con- forming with the ecological habit and growth and development law of Paris poly- phylla Smith.展开更多
[Objective] The aim was to improve part of auto-control system of aquatic seedling breeding and to lay foundation for highly-efficient and stable use of the sys- tem. [Method] The executive and drive modules of the au...[Objective] The aim was to improve part of auto-control system of aquatic seedling breeding and to lay foundation for highly-efficient and stable use of the sys- tem. [Method] The executive and drive modules of the auto-control system were im- proved, after which real-time monitor, data display and alarm modules were tested on time. [Result] In the test, for the improved system, stability and sensitivity were enhanced; different components matched better; transmitting electric resistanc6 was reduced. [Conclusion] The research provides references for auto-control of water temperature and dissolved oxygen, and faults clearing in aquatic seedling breeding.展开更多
[Objective] The aim was to seek the optimal choice of hormones,induced temperature,and initial feed for cultivating loach.[Method] Different hormones were injected to Ioaches for artificial reproduction in water at di...[Objective] The aim was to seek the optimal choice of hormones,induced temperature,and initial feed for cultivating loach.[Method] Different hormones were injected to Ioaches for artificial reproduction in water at different temperatures.During cultivation,varied initial feeds were put in to explore the optimal choice of hormones,temperature and initial feed.[Result] According to the test,fecundity of female loach is directly proportional to gonad maturity,namely,the higher gonad maturity,the more fecundity.For example,for a female loach whose maturity coefficient was 24％,absolute and relative fecundity rates were 62 142 and 990.In contrast,for a female whose maturity coefficient was 9％,the rates were 18 207 and 367,respectively.The induced effect differs upon hormones and the test demonstrated that LRH-A2 is better in improving fertility rate compared with HCG.For example,the fertility rate in the group with HCG was 78.84 and in the group with LRH-A2 was 83.04％.It is notable that the mixture of the two at a certain ratio would enhance induced effect and the fertility rate can be as high as 89.17％.With temperature in a certain range,the higher temperature,the better induced effect.The test indicated that water temperature at （30±0.5） ℃ is an optimal one and the effect horizon is 6-8 h.In group 4,induced rate was 93.33％ and the fertility rate was 89.26％.The research indicated that the survival rate was the highest if wheel animalcule,dominated by Brachionus calyciflorus and Brachionus angularis,was taken as an initial feed,followed by artemia nauplii and corpuscule.In addition,loach fry in the group with yolk and wheel animalcule grew about 1.21 times than the group with only yolk,1.04 times than the group with only fairy shrimp,and 1.1 times than the group with fairy shrimp.[Conclusion] The research provides scientific references for scale loach farming.展开更多
Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapi...Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.展开更多
[Objective] This study was to produce plant seeds on a large scale via sterile germination of the capsules of Dendrobium chrysotoxum and rapid propagation technique.[Method] A large amount of protocorm-like bodies pro...[Objective] This study was to produce plant seeds on a large scale via sterile germination of the capsules of Dendrobium chrysotoxum and rapid propagation technique.[Method] A large amount of protocorm-like bodies produced from the sterile germination of D.chrysotoxum capsules,were transferred to four different kinds of bud induction media to obtain the optimal media suitable for plantlet differentiation and growth;and then with N6 as basic medium,1.0,1.5 and 2.0 mg/L of NAA and IAA were tested to obtain the optimal media suitable for rooting.[Results] On the medium of MS appended with 1 mg/L 6-BA ＋10% banana puree ＋ 20 g/L sucrose ＋6 g/L agar＋1 g/L AC,seed germination rate was up to 90%.The optimal medium for differentiation of D.chrysotoxum protocorm-like bodies was N6＋ 2 mg/L NAA ＋ 0.5 mg/L 6-BA ＋10% banana puree ＋ 20 g/L sucrose ＋ 0.5 g/L peptone ＋ 5.8 g/L agar ＋0.5 g/L AC,grown from which the plantlets were even and orderly in height;and the optimal medium for rooting was N6＋1.5 mg/L NAA ＋10% banana puree ＋ 20 g/L sucrose ＋ 5.8 g/L agar ＋1 g/L AC,grown from which the plantlets developed more,robust and orderly roots,and their leaves were in dark green color.[Conclusion] Our results provided reference for the rapid propagation of D.chrysotoxum.展开更多
By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety diff...By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.展开更多
In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were...In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were compared in terms of explant propagation coefficient, seedling fresh weight,contents of anthocyanins and chlorophyll, and photosynthetic characteristics. The results showed that an inoculation density of 40 explants/L was suitable for the micropropagation of ’Benihope’ strawberry seedlings in the TIB. The propagation coefficient, fresh weight, photosynthetic rate, stomatal conductance, transpiration coefficient, and the contents of total anthocyanins and chlorophyll of seedlings micropropagated in the TIB were significantly higher than those of the seedlings micropropagated by the conventional method. In conclusion, the TIB method was superior to the conventional method in the micropropagation of strawberry seedlings.The negative reciprocity did not occur between anthocyanins and chlorophyll in strawberry seedlings cultured under forced ventilation such as TIB.展开更多
基金Supported by Central Financial Forestry Science and Technology Promotion project([2014]XT003)~~
文摘This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, increasing income of foresters and protecting ecological environment. Three types of fores{ land, i.e., the evergreen forest land （China fir forest）, deciduous forest land （walnut forest） and shrub land （honey suckle forest.） were selected, and seeds and cut rhizomes of Paris polyphylla Smith produced in Bamian Mountain, Hunan, were used in the study on understory seedling raising and cultivation techniques. The results showed that： when using different propagation materials, seed propagation could be adopted to reduce the consumption of wild resources and provide a large number of seedlings, and was beneficial to large-scale planting; and when conducing seedling and plantlet raising followed by transplanting and direct seeding and planting using different propagation materials in large-scale understory cultivation, direct seeding/ planting was appropriate for saving save production cost with the advantage of early production. Among the 3 different types of forest land, the best choice was broad- leaved forest land, which could achieve better economic benefit because of con- forming with the ecological habit and growth and development law of Paris poly- phylla Smith.
文摘[Objective] The aim was to improve part of auto-control system of aquatic seedling breeding and to lay foundation for highly-efficient and stable use of the sys- tem. [Method] The executive and drive modules of the auto-control system were im- proved, after which real-time monitor, data display and alarm modules were tested on time. [Result] In the test, for the improved system, stability and sensitivity were enhanced; different components matched better; transmitting electric resistanc6 was reduced. [Conclusion] The research provides references for auto-control of water temperature and dissolved oxygen, and faults clearing in aquatic seedling breeding.
文摘[Objective] The aim was to seek the optimal choice of hormones,induced temperature,and initial feed for cultivating loach.[Method] Different hormones were injected to Ioaches for artificial reproduction in water at different temperatures.During cultivation,varied initial feeds were put in to explore the optimal choice of hormones,temperature and initial feed.[Result] According to the test,fecundity of female loach is directly proportional to gonad maturity,namely,the higher gonad maturity,the more fecundity.For example,for a female loach whose maturity coefficient was 24％,absolute and relative fecundity rates were 62 142 and 990.In contrast,for a female whose maturity coefficient was 9％,the rates were 18 207 and 367,respectively.The induced effect differs upon hormones and the test demonstrated that LRH-A2 is better in improving fertility rate compared with HCG.For example,the fertility rate in the group with HCG was 78.84 and in the group with LRH-A2 was 83.04％.It is notable that the mixture of the two at a certain ratio would enhance induced effect and the fertility rate can be as high as 89.17％.With temperature in a certain range,the higher temperature,the better induced effect.The test indicated that water temperature at （30±0.5） ℃ is an optimal one and the effect horizon is 6-8 h.In group 4,induced rate was 93.33％ and the fertility rate was 89.26％.The research indicated that the survival rate was the highest if wheel animalcule,dominated by Brachionus calyciflorus and Brachionus angularis,was taken as an initial feed,followed by artemia nauplii and corpuscule.In addition,loach fry in the group with yolk and wheel animalcule grew about 1.21 times than the group with only yolk,1.04 times than the group with only fairy shrimp,and 1.1 times than the group with fairy shrimp.[Conclusion] The research provides scientific references for scale loach farming.
文摘Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.
文摘[Objective] This study was to produce plant seeds on a large scale via sterile germination of the capsules of Dendrobium chrysotoxum and rapid propagation technique.[Method] A large amount of protocorm-like bodies produced from the sterile germination of D.chrysotoxum capsules,were transferred to four different kinds of bud induction media to obtain the optimal media suitable for plantlet differentiation and growth;and then with N6 as basic medium,1.0,1.5 and 2.0 mg/L of NAA and IAA were tested to obtain the optimal media suitable for rooting.[Results] On the medium of MS appended with 1 mg/L 6-BA ＋10% banana puree ＋ 20 g/L sucrose ＋6 g/L agar＋1 g/L AC,seed germination rate was up to 90%.The optimal medium for differentiation of D.chrysotoxum protocorm-like bodies was N6＋ 2 mg/L NAA ＋ 0.5 mg/L 6-BA ＋10% banana puree ＋ 20 g/L sucrose ＋ 0.5 g/L peptone ＋ 5.8 g/L agar ＋0.5 g/L AC,grown from which the plantlets were even and orderly in height;and the optimal medium for rooting was N6＋1.5 mg/L NAA ＋10% banana puree ＋ 20 g/L sucrose ＋ 5.8 g/L agar ＋1 g/L AC,grown from which the plantlets developed more,robust and orderly roots,and their leaves were in dark green color.[Conclusion] Our results provided reference for the rapid propagation of D.chrysotoxum.
基金Supported by Youth Science Foundation of Guangxi ( Guikeqing0832060)S&T Development Project from Guangxi Academy of Agricultural Sciences(2006006)~~
文摘By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.
基金Supported by the Innovation Platform Open Fund of Education Department in Hunan Province (18K100)。
文摘In this study, we employed a temporary immersion bioreactor(TIB)system for the micropropagation of strawberry seedlings. The TIB method and the conventional method for the micropropagation of strawberry seedlings were compared in terms of explant propagation coefficient, seedling fresh weight,contents of anthocyanins and chlorophyll, and photosynthetic characteristics. The results showed that an inoculation density of 40 explants/L was suitable for the micropropagation of ’Benihope’ strawberry seedlings in the TIB. The propagation coefficient, fresh weight, photosynthetic rate, stomatal conductance, transpiration coefficient, and the contents of total anthocyanins and chlorophyll of seedlings micropropagated in the TIB were significantly higher than those of the seedlings micropropagated by the conventional method. In conclusion, the TIB method was superior to the conventional method in the micropropagation of strawberry seedlings.The negative reciprocity did not occur between anthocyanins and chlorophyll in strawberry seedlings cultured under forced ventilation such as TIB.
