苦草(Vallisneria)隶属于水鳖科(Hydrocharitaceae),是水域生态修复的先锋物种。为了对苦草属植物进行比较全面的SSR信息分析,本研究基于RAD-seq (restriction-site associated DNA sequencing)简化基因组测序技术获得了苦草的简化基因...苦草(Vallisneria)隶属于水鳖科(Hydrocharitaceae),是水域生态修复的先锋物种。为了对苦草属植物进行比较全面的SSR信息分析,本研究基于RAD-seq (restriction-site associated DNA sequencing)简化基因组测序技术获得了苦草的简化基因组序列信息,并开发了SSR分子标记与引物设计。在所得序列中共检测到366个SSR位点,成功设计出引物的有355个,其中二碱基重复基序数量最多,占总数的59.56%,通过筛选和验证,最终确定了23对具有多态性的SSR引物。Genepop软件分析结果显示,这23个位点的等位基因数均值为3.26,多态性高且不连锁(P<0.05);4个位点在多数群体中偏离哈迪温伯格平衡(P<0.01)且纯合子与杂合子比例相当(观测杂合度均值0.460),近交系数高(Fis均值0.880),可能是因为单个群体采样集中,且由于苦草属植物同时具备无性、有性两种繁殖方式中的无性繁殖所致。展开更多
Clonal integration benefits clonal plants by buffering environmental stress and increasing resource extraction efficiency.However,the number of connected ramet generations that benefit from clonal integration in a clo...Clonal integration benefits clonal plants by buffering environmental stress and increasing resource extraction efficiency.However,the number of connected ramet generations that benefit from clonal integration in a clonal system has received relatively little attention.A pot experiment was conducted to evaluate the extent of physiological integration within the clonal system of Vallisneria natans consisting of a mother ramet and three sequentially connected offspring ramets.Mother ramets were grown in full sunlight,and offspring ramets were heavily shaded with limited light availability.Stolons between mother ramets and offspring ramets were severed or connected,but connection among the three offspring ramets remained.The photosynthetic ability of unshaded mother ramets of V.natans was significantly enhanced,but their biomass accumulation was greatly reduced when connected to shaded offspring ramets.Clonal integration significantly increased biomass accumulation,C and N availabilities,extracellular enzyme activities and microbial biomass of the first ramet generation(adjacent ramet),but not later ramet generations.Our results indicate that support from the mother ramet of V.natans may be limited to the adjacent offspring ramet in a clonal system under severe light stress,implying an effect of ramet generation.Our results contribute to a better understanding of the hierarchy and segmentation of clonal plants.These findings suggest that the extent of clonal integration plays a vital role in ecological interactions of the ramet population.展开更多
文摘苦草(Vallisneria)隶属于水鳖科(Hydrocharitaceae),是水域生态修复的先锋物种。为了对苦草属植物进行比较全面的SSR信息分析,本研究基于RAD-seq (restriction-site associated DNA sequencing)简化基因组测序技术获得了苦草的简化基因组序列信息,并开发了SSR分子标记与引物设计。在所得序列中共检测到366个SSR位点,成功设计出引物的有355个,其中二碱基重复基序数量最多,占总数的59.56%,通过筛选和验证,最终确定了23对具有多态性的SSR引物。Genepop软件分析结果显示,这23个位点的等位基因数均值为3.26,多态性高且不连锁(P<0.05);4个位点在多数群体中偏离哈迪温伯格平衡(P<0.01)且纯合子与杂合子比例相当(观测杂合度均值0.460),近交系数高(Fis均值0.880),可能是因为单个群体采样集中,且由于苦草属植物同时具备无性、有性两种繁殖方式中的无性繁殖所致。
基金the Special Foundation of National Science and Technology Basic Research(2013FY112300)the National Natural Science Foundation of China(31900281).
文摘Clonal integration benefits clonal plants by buffering environmental stress and increasing resource extraction efficiency.However,the number of connected ramet generations that benefit from clonal integration in a clonal system has received relatively little attention.A pot experiment was conducted to evaluate the extent of physiological integration within the clonal system of Vallisneria natans consisting of a mother ramet and three sequentially connected offspring ramets.Mother ramets were grown in full sunlight,and offspring ramets were heavily shaded with limited light availability.Stolons between mother ramets and offspring ramets were severed or connected,but connection among the three offspring ramets remained.The photosynthetic ability of unshaded mother ramets of V.natans was significantly enhanced,but their biomass accumulation was greatly reduced when connected to shaded offspring ramets.Clonal integration significantly increased biomass accumulation,C and N availabilities,extracellular enzyme activities and microbial biomass of the first ramet generation(adjacent ramet),but not later ramet generations.Our results indicate that support from the mother ramet of V.natans may be limited to the adjacent offspring ramet in a clonal system under severe light stress,implying an effect of ramet generation.Our results contribute to a better understanding of the hierarchy and segmentation of clonal plants.These findings suggest that the extent of clonal integration plays a vital role in ecological interactions of the ramet population.
