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胶孢炭疽菌及两种疫霉菌对苯酰菌胺的敏感性与β-微管蛋白氨基酸突变的相关性分析 被引量:3
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作者 邓维萍 梅馨月 +7 位作者 杨敏 陈磊 毕扬 蔡萌 王海燕 阿普前 何霞红 朱书生 《农药学学报》 CAS CSCD 北大核心 2012年第2期143-150,共8页
选择对多菌灵、乙霉威和苯酰菌胺具有不同敏感性的胶孢炭疽菌Colletotrichum gloeosporioides、辣椒疫霉菌Phytophthora capsici及恶疫霉菌P.cactorum,采用菌丝生长速率抑制法及氨基酸序列比对法分析了其β-微管蛋白氨基酸突变与敏感性... 选择对多菌灵、乙霉威和苯酰菌胺具有不同敏感性的胶孢炭疽菌Colletotrichum gloeosporioides、辣椒疫霉菌Phytophthora capsici及恶疫霉菌P.cactorum,采用菌丝生长速率抑制法及氨基酸序列比对法分析了其β-微管蛋白氨基酸突变与敏感性的关系。结果表明,胶孢炭疽菌对苯酰菌胺、多菌灵和乙霉威的敏感性与β-微管蛋白198位或200位氨基酸突变有关:对多菌灵敏感、对苯酰菌胺和乙霉威不敏感的胶孢炭疽菌β-微管蛋白氨基酸198位为谷氨酸(E),200位为苯丙氨酸(F);对多菌灵已产生抗性而对苯酰菌胺和乙霉威不敏感的菌株,其β-微管蛋白氨基酸200位由苯丙氨酸(F)突变为了酪氨酸(Y);对多菌灵高抗、对苯酰菌胺和乙霉威敏感的菌株其β-微管蛋白氨基酸198位由谷氨酸(E)突变为了丙氨酸(A)。辣椒疫霉菌和恶疫霉菌对苯酰菌胺敏感,对多菌灵和乙霉威均不敏感。检测疫霉菌菌株β-微管蛋白未发现氨基酸突变,但发现其β-微管蛋白氨基酸在196~200位与胶孢炭疽菌差异较大,这可能是导致苯酰菌胺仅对疫霉菌有抑制效果的原因。 展开更多
关键词 苯酰菌胺 疫霉 胶孢炭疽 Β-微管蛋白 敏感性
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苯酰菌胺在黄瓜及土壤中的残留动态 被引量:2
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作者 张春荣 平立凤 +4 位作者 赵华 何红梅 胡秀卿 吴珉 李振 《浙江农业学报》 CSCD 北大核心 2011年第6期1177-1181,共5页
建立了气相色谱(GC)分析黄瓜及土壤中苯酰菌胺残留的方法。样品经乙腈提取,碱性氧化铝柱净化后用气相色谱仪的电子捕获检测器(GC/ECD)检测。结果表明:苯酰菌胺在0.002~0.100 mg.L-1范围线性关系良好,相关系数为1;苯酰菌胺在黄瓜及土壤... 建立了气相色谱(GC)分析黄瓜及土壤中苯酰菌胺残留的方法。样品经乙腈提取,碱性氧化铝柱净化后用气相色谱仪的电子捕获检测器(GC/ECD)检测。结果表明:苯酰菌胺在0.002~0.100 mg.L-1范围线性关系良好,相关系数为1;苯酰菌胺在黄瓜及土壤中的最低检出质量分数为0.01 mg.kg-1;黄瓜中苯酰菌胺的平均添加回收率为89.4%~96.9%,变异系数为4.7%~10.9%;土壤中苯酰菌胺的平均添加回收率为90.1%~97.8%,变异系数为2.9%~4.5%。苯酰菌胺在黄瓜及土壤中的消解动态符合一级动力学方程,在黄瓜中的消解半衰期为1.3~3.3 d,土壤中的消解半衰期为1.6~5.6 d。 展开更多
关键词 苯酰菌胺 黄瓜 土壤 残留 气相色谱法
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苯酰菌胺原药和水分散粒剂的高效液相色谱分析方法研究 被引量:1
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作者 陈慧萍 孟璨 +2 位作者 马杜康 赵鹏跃 黄啟良 《现代农药》 CAS 2021年第3期17-19,25,共4页
笔者采用高效液相色谱法,以乙腈+水为流动相,使用Agilent ZORBAX SB-C18为填料的不锈钢色谱柱和紫外-可见检测器,在240 nm波长下对苯酰菌胺原药和锰锌·苯酰菌胺水分散粒剂试样中的有效成分苯酰菌胺进行分离和定量分析。结果表明,... 笔者采用高效液相色谱法,以乙腈+水为流动相,使用Agilent ZORBAX SB-C18为填料的不锈钢色谱柱和紫外-可见检测器,在240 nm波长下对苯酰菌胺原药和锰锌·苯酰菌胺水分散粒剂试样中的有效成分苯酰菌胺进行分离和定量分析。结果表明,苯酰菌胺的线性回归方程为y=8.2968 x+51.66,相关系数为1.0000;苯酰菌胺原药和锰锌·苯酰菌胺水分散粒剂试验中有效成分苯酰菌胺的标准偏差分别为0.15%和0.03%;苯酰菌胺水分散粒剂的平均回收率为101.50%。该方法灵敏高、操作简便快速,适用于苯酰菌胺原药及制剂产品的含量检测。 展开更多
关键词 苯酰菌胺 原药 水分散粒剂 高效液相色谱 分析
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75%苯酰菌胺+代森锰锌可分散粒剂防治葡萄霜霉病田间药效试验 被引量:1
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作者 孟新房 赵亚荣 薛源清 《陕西农业科学》 2013年第5期91-92,100,共3页
田间药效试验表明,75%苯酰菌胺+代森锰锌可分散粒剂防治葡萄霜霉病防效显著,对作物安全无药害,是防治葡萄霜霉病的良好药剂。
关键词 75%苯酰菌胺+代森锰锌可分散粒剂 葡萄霜霉病 防治效果
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75%苯酰菌胺+代森锰锌可分散粒剂防治马铃薯晚疫病试验研究 被引量:1
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作者 于凤海 《农业开发与装备》 2015年第1期77-78,共2页
通过一年的药效试验,75%苯酰菌胺+代森锰锌可分散粒剂对马铃薯晚疫病防效好,对作物生长安全,增产显著。
关键词 75%苯酰菌胺+代森锰锌可分散粒剂 马铃薯晚疫病 防病效果
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75%苯酰菌胺+代森锰锌可分散粒剂防治马铃薯晚疫病试验研究
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作者 王占宇 《吉林农业(下半月)》 2016年第3期88-88,共1页
马铃薯晚疫病是毁灭性病害,严重制约着甘南县马铃薯产业的发展,为了筛选出高效低毒的防治药剂,甘南县对引进的防治新产品进行试验。通过试验了解到75%苯酰菌胺+代森锰锌可分散粒剂是治疗马铃薯霜霉病、疫霉病、腐霉病的特效杀菌农药,对... 马铃薯晚疫病是毁灭性病害,严重制约着甘南县马铃薯产业的发展,为了筛选出高效低毒的防治药剂,甘南县对引进的防治新产品进行试验。通过试验了解到75%苯酰菌胺+代森锰锌可分散粒剂是治疗马铃薯霜霉病、疫霉病、腐霉病的特效杀菌农药,对马铃薯晚疫病防治效果较好。 展开更多
关键词 苯酰菌胺+代森锰锌 马铃薯晚疫病 防治效果 经济效益
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杀菌剂苯酰菌胺的合成
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作者 彭军 刘世勇 《广东化工》 CAS 2021年第5期44-45,19,共3页
本文对苯酰菌胺的合成工艺进行研究,确定了五步法的合成路线。以3,5-二氯-4-甲基苯甲酸、3-氨基-3-甲基-1-戊炔、三氯异氰尿酸等原料,经酰氯化、酰胺化、异构合环、氯化、水解成功合成苯酰菌胺。提供一种易于工业化的生产工艺,按此工艺... 本文对苯酰菌胺的合成工艺进行研究,确定了五步法的合成路线。以3,5-二氯-4-甲基苯甲酸、3-氨基-3-甲基-1-戊炔、三氯异氰尿酸等原料,经酰氯化、酰胺化、异构合环、氯化、水解成功合成苯酰菌胺。提供一种易于工业化的生产工艺,按此工艺合成了五批次产品,结果证明工艺稳定,产品含量大于98%,总收率达到70%,该工艺简单经济,条件温和,适合工业化生产。 展开更多
关键词 苯酰菌胺 合成
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Variations of phyllosphere microorganisms in asymptomatic and tobacco brown spot leaves before and after spraying 12% difenoconazole + fluxapyroxad SC
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作者 LI Tong WANG Hancheng +6 位作者 YE Guo WANG Qing NGANGUEM NZALLE Yranney Brice WANG Feng CAI Liuti FENG Ruichao ZHANG Songbai 《农药学学报》 CAS CSCD 北大核心 2024年第5期932-948,共17页
12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight... 12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology. 展开更多
关键词 tobacco brown spot DIFENOCONAZOLE fluxapyroxad microorganism communities diversity high-throughput sequencing
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Acute and joint toxicity of three agrochemicals to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles 被引量:4
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作者 Li WEI Wei-Wei SHAO +3 位作者 Guo-Hua DING Xiao-Li FAN Miao-Ling YU Zhi-Hua LIN 《Zoological Research》 CAS CSCD 北大核心 2014年第4期272-279,共8页
We studied acute and joint toxicity of three different agrochemicals (chlorantraniliprole, flubendiamide-abamectin and penoxsulam) to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles with the method of stabi... We studied acute and joint toxicity of three different agrochemicals (chlorantraniliprole, flubendiamide-abamectin and penoxsulam) to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles with the method of stability water tests. Results showed that the three agrochemicals increased tadpole mortality. For acute toxicity, the LC50 values after 24, 48 and 72 h of chlorantraniliprole, flubendiamide-abamectin and penoxsulam exposure were 5.37, 4.90 and 4.68 mg/L; 0.035, 0.025 and 0.021 rag/L; 1.74, 1.45 and 1.29 mg/L, respectively. The safety concentrations (SC) of chlorantraniliprole, fiubendiamide-abamectin and penoxsulam to the tadpoles were 1.23, 0.30 and 0.003 mg/L, respectively. Based on these findings, chlorantraniliprole and penoxsulam were moderately toxic, while flubendiamide-abamectin was highly toxic. All pairwise joint toxicity tests showed moderate toxicity. The LCs0 values after 24, 48 and 72 h of exposure were 7.08, 6.61 and 6.03 mg/L for chlorantra- niliprole+penoxsulam, with corresponding values of 2.455, 2.328 and 2.183 mg/L for chlorantraniliprole+flubendiamide-abamectin, and 1.132, 1.084 and 1.050 mg/L for penoxsulam+flubendiamide-abamectin, with safe concentrations of 1.73, 0.63 and 0.30 mg/L, respectively. For toxic evaluations of pairwise combinations of the three agrochemicals, only the joint toxicity of chlorantraniliprole and flubendiamide-abamectin after 24 h was found to be synergistic, whereas all other tests were antagonistic. Our findings provide valuable information on the toxic effects of agrochemicals on amphibians and how various types of agrochemicals can be reasonably used in agricultural areas. 展开更多
关键词 Agrochemical Acute toxicity Joint toxicity LCs0 Safe concentration Hoplobatrachus chinensis
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