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苯系物降解菌的驯化培养及纯化筛选
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作者 陈文聪 徐悦 +1 位作者 王础 阎松 《能源与环境》 2024年第2期138-141,共4页
对来自大连春柳污泥处理厂的活性污泥进行驯化培养,通过控制苯浓度、培养基种类等条件,结合气相色谱法测定,从中纯化筛选出生长情况好、苯降解率高的BTEX4号和BTEX6号菌株,以分子生物学和生物信息学方法对BTEX4号和BTEX6号菌株进行系统... 对来自大连春柳污泥处理厂的活性污泥进行驯化培养,通过控制苯浓度、培养基种类等条件,结合气相色谱法测定,从中纯化筛选出生长情况好、苯降解率高的BTEX4号和BTEX6号菌株,以分子生物学和生物信息学方法对BTEX4号和BTEX6号菌株进行系统发育分析和种属鉴定,结果表明:BTEX4(4号菌株)属于肺炎克雷伯菌,BTEX6(6号菌株)属于木霉菌属。鉴定出来的6号菌株可能成为1种苯高效降解菌用于生产中。 展开更多
关键词 苯降解菌 驯化 种鉴定 16S RDNA
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苯并[a]芘降解菌在不同土壤无机胶体表面的吸附研究
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作者 甘信宏 滕应 +3 位作者 牟婷婷 刘国强 徐建 骆永明 《生态与农村环境学报》 CAS CSCD 北大核心 2023年第1期115-122,共8页
基于等温吸附实验,结合扩展DLVO(Ex-DLVO)理论、傅里叶红外光谱(FTIR)等方法与手段,探究高岭石、蒙脱石和赤铁矿胶体对苯并[a]芘的高效降解菌噬氨副球菌(Paracoccus aminovorans)HPD-2的界面相互作用机制。结果表明,3种典型土壤无机胶... 基于等温吸附实验,结合扩展DLVO(Ex-DLVO)理论、傅里叶红外光谱(FTIR)等方法与手段,探究高岭石、蒙脱石和赤铁矿胶体对苯并[a]芘的高效降解菌噬氨副球菌(Paracoccus aminovorans)HPD-2的界面相互作用机制。结果表明,3种典型土壤无机胶体对菌株HPD-2的等温吸附曲线符合Langmuir吸附模型,其理论最大吸附量由大到小为赤铁矿>>蒙脱石>高岭石。Ex-DLVO理论计算结果表明,赤铁矿对菌株HPD-2细胞的吸附过程是自发的,而高岭石和蒙脱石对降解菌的吸附均需要越过能障;胶体颗粒与细胞之间的静电力作用在吸附过程中起到关键作用,其产生的作用能远高于范德华力和疏水作用力。FTIR结果表明,高岭石和蒙脱石对菌株HPD-2的作用力主要为细胞表面蛋白质构型的变化,相互作用较弱。除氢键外,赤铁矿与菌株HPD-2发生吸附的作用力还形成了较强的P—OFe化学键。研究结果阐明了不同性质无机矿物胶体与菌株HPD-2细胞间的界面作用机制,为研究无机矿物对降解微生物活性和微生物在无机矿物表面定殖的影响机制提供理论依据。 展开更多
关键词 土壤无机胶体 并[a]芘降解 扩展DLVO理论 界面作用
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Proteome Analysis of the Adaptation of a Phenol-Degrading Bacterium Acinetobacter sp. EDP3 to the Variation of Phenol Loadings 被引量:3
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作者 GENG Anli LIM Ci Ji 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2007年第6期781-787,共7页
Strain EDP3 was isolated from an industrial-activated sludge. It belonged to the gamma group of Pro-teobacteria with an identity of 97.0% to Acinetobacter calcoaceticus according to the 16S rRNA gene sequences. It can... Strain EDP3 was isolated from an industrial-activated sludge. It belonged to the gamma group of Pro-teobacteria with an identity of 97.0% to Acinetobacter calcoaceticus according to the 16S rRNA gene sequences. It can tolerate up to 1000mg·L^-1 phenol at room temperature with a much longer lag phase. This indicates that higher phenol concentration has induced some physiological and genotypic changes in the bacterium. The aim of this study is, therefore, to investigate these responses to phenol concentration variations in strain EDP3. Proteome analysis is conducted by means of a two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was conducted to obtain a deeper insight into the adaptive responses inside the bacterium. Comparative analysis of the proteome profiles of strain EDP3. grown in 400mg·L^-1 and 1000mg·L^-1 phenol allowed us to identify that among all the proteins up-regulated under the higher phenol concentration, oxidative stress proteins were dominant. The synthesis of a heat shock protein, 60000 chaperonin GroEL, was also amplified. In addition, the expression of one membrane protein, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) type sugar transporter, was found up-regulated. The inhibition of adenosine 5'-triphosphate (ATP) and RNA/protein synthesis was also observed. 展开更多
关键词 ADAPTATION phenol-degrading bacteria Acinetobacter sp. PROTEOME
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Isolation and characteristics of Arthrobacter sp.strain CW-1 for biodegradation of PAEs 被引量:5
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作者 MAHMOOD Qaisar 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2007年第9期1469-1474,共6页
Isolation of new bacterial strains and recognition of their metabolic activities are highly desirable for sustainability of natural ecosystems. Biodegradation of dimethyl phthalate (DMP) under anoxic conditions has ... Isolation of new bacterial strains and recognition of their metabolic activities are highly desirable for sustainability of natural ecosystems. Biodegradation of dimethyl phthalate (DMP) under anoxic conditions has been shown to occur as a series of sequential steps using strain CW-1 isolated from digested sludge of Sibao Wastewater Treatment Plant in Hangzhou, China. The microbial colony on LB medium was yellowish, 3-5 mm in diameter, convex in the center, and embedded in mucous externally. The individual cells of strain CW-1 are irregular rods, measuring (0.6-0.7)×(0.9-1.0) pm, V-shaped, with clubbed ends, Gram positive and without any filaments. 16S rDNA ( 1438 bp) sequence analysis showed that the strain was related to Arthrobacter sp. CW-1 and can degrade PAEs utilizing nitrate as electron acceptor, but cannot mineralize DMP completely. The degradation pathway was recommended as: dimethyl phthalate (DMP)→monomethyl phthalate (MMP)--,phthalic acid (PA). DMP biodegradation was a first order reaction with degradation rate constant of 0.3033 d 1 and half-life 2.25 d. The DMP conversion to PA by CW-1 could be described by using sequential kinetic model. 展开更多
关键词 Denitrifying bacteria Arthrobacter sp. strain CW-1 Dimethyl phthalate (DMP) Phthalic acid (PA) DMP degradation pathway
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Effects of a Biosurfactant and a Synthetic Surfactant on Phenanthrene Degradation by a Sphingomonas Strain 被引量:10
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作者 PEI Xiao-Hong ZHAN Xin-Hua +2 位作者 WANG Shi-Mei LIN Yu-Suo ZHOU Li-Xiang 《Pedosphere》 SCIE CAS CSCD 2010年第6期771-779,共9页
A novel phenanthrene (PHE)-degrading strain named as Sphingomonas sp.GF2B was isolated and identified from a farmland soil.Effects of a synthetic surfactant (Tween-80) and a rhamnolipid biosurfactant on PHE degradatio... A novel phenanthrene (PHE)-degrading strain named as Sphingomonas sp.GF2B was isolated and identified from a farmland soil.Effects of a synthetic surfactant (Tween-80) and a rhamnolipid biosurfactant on PHE degradation by Sphin- gomonas sp.GF2B were investigated at different concentrations of the surfactants.The results showed that Sphingomonas sp.GF2B was able to mineralize up to 83.6% of PHE within 10 days without addition of surfactants.The addition of Tween-80 to the reaction medium inhibited greatly PHE biodegradation,with only 33.5% of PHE degraded.However,the biosurfactant facilitated PHE biodegradation,with up to 99.5% of PHE degraded.The preferential utilization of PHE as a carbon source and the enhanced solubility of PHE by the biosurfactant were likely responsible for the higher biodegra- dation efficiency of PHE in the presence of the biosurfactant.Therefore,it could be concluded that the application of the biosurfactant to PHE-contaminated soils was perhaps a feasible way to facilitate the PHE biodegradation. 展开更多
关键词 biodegradation contaminated soils RHAMNOLIPID solubility TWEEN-80
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Biodegradation of Benzo[a]pyrene by Arthrobacter oxydans B4 被引量:5
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作者 PENG Hui YIN Hua +4 位作者 DENG Jun YE Jin-Shao CHEN Shuo-Na HE Bao-Yan ZHANG Na 《Pedosphere》 SCIE CAS CSCD 2012年第4期554-561,共8页
A bacterial strain, Arthrobacter oxydans (B4), capable of degrading benzo[a]pyrene (BaP) in water body, was isolated from a polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as... A bacterial strain, Arthrobacter oxydans (B4), capable of degrading benzo[a]pyrene (BaP) in water body, was isolated from a polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as reaction time, pH value, temperature and organic nutrients, on BaP biodegradation by the strain B4 were studied. After 5 d treatment, the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1, compared to the initial concentration of 1.000 mg L-1. There was a process of acid formation during the degradation with pH falling from initial 7.01 to 4.61 at 5 d, so keeping the water body under slightly alkaline condition was propitious to BaP degradation. Strain B4 efficiently degraded BaP at 20 to 37 ~C with addition of organic nutrients. The biodegradation and transformation of BaP mainly occurred on cell surfaces, and extracellular secretions played an important role in these processes. Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth. Furthermore, mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system. 展开更多
关键词 DEGRADATION MECHANISM METABOLITES microbial transformation polycyclic aromatic hydrocarbon
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