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四个杂交粳稻的蛋白质、赖氨酸、直链淀粉含量和MDH活性测定
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作者 李建粤 蔡福根 胡靖华 《上海师范大学学报(自然科学版)》 1994年第1期80-84,共5页
本文分析比较了正在大面积推广种植的杂交粳稻寒优1027以及3个新选育的杂交粳稻8优161、寒优195和寒优356的蛋白质含量、赖氨酸含量、赖氨酸相对合量、直挂淀粉含量和苹果田脱氢酶(MDH)活性.结果显示了3个新选育... 本文分析比较了正在大面积推广种植的杂交粳稻寒优1027以及3个新选育的杂交粳稻8优161、寒优195和寒优356的蛋白质含量、赖氨酸含量、赖氨酸相对合量、直挂淀粉含量和苹果田脱氢酶(MDH)活性.结果显示了3个新选育的杂交粳稻分别在不同的方面胜过对照寒优1027.寒优195的直链淀粉合量(12.1%)明显低于对照寒优1027(17.0%),赖氨酸的相对合量(3.68%)高于对照寒优1027(3.18%),统计分析两方面的差异都达极著水平(P<0.01);8优161的蛋白质含量(9.69%)比对照寒优1027(8.78%)高11.1%,统计分析差异达极显著(P<0.01);寒优3564白质含量(9.15%)比对照寒优1027高.出5.5%,两者差异也达极显著(P<0.01),而且寒优356在苗期的MDH活性高于对照寒优1027,统计分析两者差异显著(P<0.05),这表明寒优356在生长后期的每生日穗总粒数,每穗实粒数和每实粒重可能会超过对照寒优1027.建议有关部门在今后的水稻生产中,可根据各自不同的需要,推广利用不同的杂交粳稻. 展开更多
关键词 杂交粳稻 蛋白质含量 赖氨酸相对合量 直链淀粉含量 苹果酸(MDH)活性
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嗜酸氧化亚铁硫杆菌ATCC23270周质蛋白的选择性提取及差异表达 被引量:3
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作者 夏金兰 王晶 +4 位作者 张倩 张瑞永 张成桂 欧阳叙东 邱冠周 《中南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2009年第4期845-850,共6页
通过考察萃取时间、高渗液与低渗液的摩尔比以及总的渗透液体积对细胞质苹果酸脱氢酶的比活性(相对于细胞蛋白质总量)的影响,建立选择性提取Acidithiobacillus ferrooxidans ATCC23270周质蛋白的渗透休克实验方法;结合透射电镜和双向电... 通过考察萃取时间、高渗液与低渗液的摩尔比以及总的渗透液体积对细胞质苹果酸脱氢酶的比活性(相对于细胞蛋白质总量)的影响,建立选择性提取Acidithiobacillus ferrooxidans ATCC23270周质蛋白的渗透休克实验方法;结合透射电镜和双向电泳实验方法,分析渗透处理前后细胞的形态和不同生长期的细胞周质空间蛋白表达差异。研究结果表明,合适的渗透休克实验条件为:细胞质量为0.1g(湿重),渗透液的总体积为10mL,渗透液的作用时间为15min,高渗液与低渗液的摩尔比为1:1;在高渗环境下,细胞收缩,周质空间变小;在低渗液环境下,细胞周质空间膨胀,外膜被胀破,周质空间内蛋白释放出来;双向电泳显示周质蛋白总体上以小相对分子质量蛋白为主,在不同的生长时期内的周质蛋白表达存在差异。 展开更多
关键词 ACIDITHIOBACILLUS ferrooxidans 周质蛋白 双向电泳 苹果酸脱氢酶活性 渗透休克法
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Effects of Different Preservative Treatments on Physiological Metabolism and Preservation of Sweet Cherry 被引量:3
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作者 朱向秋 陈茂全 邹昊 《Agricultural Science & Technology》 CAS 2012年第6期1373-1375,1380,共4页
[Objective] This study aimed to investigate the effects of different preservative treatments on physiological metabolism and preservation of sweet cherry. [Method] Sweet cherry (Prunus avium var. Summit) was soaked ... [Objective] This study aimed to investigate the effects of different preservative treatments on physiological metabolism and preservation of sweet cherry. [Method] Sweet cherry (Prunus avium var. Summit) was soaked into benziothiazolinone (1 000 ppm), lysozyme (500 ppm), lysozyme (500 ppm) + NPS polysaccharide (5 000 ppm) and water for 5 min, respectively. Non-treated sweet cherry was set as control. All the sweet cherries were then put into 3 mm thick PE bags and preserved at (-0.5±0.5) ℃. [Result] The results showed that the malate dehydrogenase (MDH) activity of benziothiazolinone treatment researched a significant peak on the 14 th d, while the MDH activity of Lysozyme (500 ppm), Lysozyme (500 ppm) + NPS polysaccharide (5 000 ppm) and water treatments began to increase on the 20 th d; the polyphenol oxidase (PPO) activity in various treatments showed a decreasing trend during the experiment, which researched a significant peak on the 14 th d, while that in water treatment was decreased consistently; on the 21 st d, the PPO activity in each treatment increased slowly; the titratable acid (TA) content in preservative treatments was higher than that in both water treatment and control; the soluble solid (SSC) content showed no significant difference between various treatments and between experimental treatment and control; on the 40 th d, the healthy fruit rate in preservative treatments was significantly higher than that in water treatment and control. [Conclusion] Benziothiazolinone, lysozyme and other preservatives show good effects on preservation of sweet cherry; lysozyme treatment can decrease the activity of malate dehydrogenase, maintain the relatively high content of organic acid and significantly improve the healthy fruit rate within a certain period of time. 展开更多
关键词 Sweet cherry PRESERVATION Malate dehydrogenase PRESERVATIVE Oxidase activity
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