The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected p...The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.展开更多
The eggplant (Solanummelongena L.) is widely cultivated across the world.Technology regarding the in vitro germination and preservation of eggplant pollen is important for the production of hybrid seeds.In this work,t...The eggplant (Solanummelongena L.) is widely cultivated across the world.Technology regarding the in vitro germination and preservation of eggplant pollen is important for the production of hybrid seeds.In this work,the suitable cultural medium and time for the germination of fresh and dried pollen was investigated.The results showed that a suitable medium for eggplant pollen germination was 0.5% agar+5% sucrose+0.01% boric acid+0.01% GA3,the suitable culture time was 2 h for fresh pollen and 4 h for dried pollen stored at low temperature.GA3 could induce the germination of dried and stored pollen. The better way to dry anthers was 4 h in a desiccator at 25~30℃.We also found that pollen from the second to the fourth branch has the highest germination rate. Furthermore,there were no significant differences in the seed setting rates among the different storage temperatures and storage times,whereas the number of viable seeds formed per fruit differed significantly,which was 87.3% more for pollen stored at -80℃ and fresh pollen than that stored at 4℃.This work optimized pollen storage conditions and is helpful to eggplant hybrid seed production.展开更多
基金Supported by College Students’Technology Innovation and Entrepreneurship Action Fund of Yunnan Agricultural University(2014ZKX048)~~
文摘The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.
文摘The eggplant (Solanummelongena L.) is widely cultivated across the world.Technology regarding the in vitro germination and preservation of eggplant pollen is important for the production of hybrid seeds.In this work,the suitable cultural medium and time for the germination of fresh and dried pollen was investigated.The results showed that a suitable medium for eggplant pollen germination was 0.5% agar+5% sucrose+0.01% boric acid+0.01% GA3,the suitable culture time was 2 h for fresh pollen and 4 h for dried pollen stored at low temperature.GA3 could induce the germination of dried and stored pollen. The better way to dry anthers was 4 h in a desiccator at 25~30℃.We also found that pollen from the second to the fourth branch has the highest germination rate. Furthermore,there were no significant differences in the seed setting rates among the different storage temperatures and storage times,whereas the number of viable seeds formed per fruit differed significantly,which was 87.3% more for pollen stored at -80℃ and fresh pollen than that stored at 4℃.This work optimized pollen storage conditions and is helpful to eggplant hybrid seed production.
