目的:探讨茵芍散含药血清对大鼠肝星状细胞(HSC)增殖作用的影响及其机制。方法:40只Wistar大鼠分别给予中药高、中、低剂量、0.9%氯化钠溶液、己酮可可碱灌胃,喂养1周后,腹主动脉采血,制备药物血清。设中药高、中、低剂量组,阴性对照组...目的:探讨茵芍散含药血清对大鼠肝星状细胞(HSC)增殖作用的影响及其机制。方法:40只Wistar大鼠分别给予中药高、中、低剂量、0.9%氯化钠溶液、己酮可可碱灌胃,喂养1周后,腹主动脉采血,制备药物血清。设中药高、中、低剂量组,阴性对照组,阳性对照组及空白组,药物血清干预HSC-T6后,运用流式细胞术、吖啶橙/溴化乙锭(AO-EB)染色观察细胞增殖、分析周期情况,并分别通过实时荧光定量PCR、Western blot检测β-catenin m RNA及蛋白表达情况。结果:与对照组比较,茵芍散能有效抑制HSC-T6增殖,下调β-catenin m RNA及蛋白表达(P<0.05),且具有量-效关系。结论:茵芍散可抑制HSC增殖,阻断Wnt/β-catenin信号通路对HSC的激活可能是其治疗肝纤维化的作用机制。展开更多
Heidan disease(black jaundice)is a kind of jaundice,which is caused by lingering and chronic jaundice,often with blood stasis and damp-heat,etc.The clinical symptoms of Heidan disease(black jaundice)are similar to tho...Heidan disease(black jaundice)is a kind of jaundice,which is caused by lingering and chronic jaundice,often with blood stasis and damp-heat,etc.The clinical symptoms of Heidan disease(black jaundice)are similar to those of cirrhosis caused by multiple chronic liver diseases in Western medicine.Heidan disease(black jaundice)generally belongs to yin jaundice type,and the pathogenesis is mostly related to blood stasis and dampness stagnation,often with damp-heat residue.According to Zhongjing Zhang,the prescription Xiaoshi Fanshi powder for the treatment of Heidan disease(black jaundice)is based on the understanding that the nature of Heidan disease(black jaundice)is inseparable from the two key pathological factors of dampness and blood stasis.The treatment of jaundice should be based on removing blood stasis and dampness,supplemented by soothing the liver and promoting the transportation function of spleen,removing blood stasis and harmonizing the collaterals,and promoting diuresis and reducing jaundice.In the treatment of jaundice,removing blood stasis and purging turbidity should be stressed,and powerful tonification should be used with caution.Since blood stasis and turbidity are always intermingling and often complicated with damp heat,the method of warm drying should be used with caution.For promoting blood circulation,removing blood stasis,and dredging the liver-biliarycollaterals,drasticmedicine shouldbeused withcaution.展开更多
文摘目的:探讨茵芍散含药血清对大鼠肝星状细胞(HSC)增殖作用的影响及其机制。方法:40只Wistar大鼠分别给予中药高、中、低剂量、0.9%氯化钠溶液、己酮可可碱灌胃,喂养1周后,腹主动脉采血,制备药物血清。设中药高、中、低剂量组,阴性对照组,阳性对照组及空白组,药物血清干预HSC-T6后,运用流式细胞术、吖啶橙/溴化乙锭(AO-EB)染色观察细胞增殖、分析周期情况,并分别通过实时荧光定量PCR、Western blot检测β-catenin m RNA及蛋白表达情况。结果:与对照组比较,茵芍散能有效抑制HSC-T6增殖,下调β-catenin m RNA及蛋白表达(P<0.05),且具有量-效关系。结论:茵芍散可抑制HSC增殖,阻断Wnt/β-catenin信号通路对HSC的激活可能是其治疗肝纤维化的作用机制。
基金supported by the National Natural Science Foundation of China(81403407)Special Program of the International Cooperation in Chinese Medicine of the State Administration of Traditional Chinese Medicine(China-Australia Chinese Medicine Center[Melbourne]GZYYGJ2021024)。
文摘Heidan disease(black jaundice)is a kind of jaundice,which is caused by lingering and chronic jaundice,often with blood stasis and damp-heat,etc.The clinical symptoms of Heidan disease(black jaundice)are similar to those of cirrhosis caused by multiple chronic liver diseases in Western medicine.Heidan disease(black jaundice)generally belongs to yin jaundice type,and the pathogenesis is mostly related to blood stasis and dampness stagnation,often with damp-heat residue.According to Zhongjing Zhang,the prescription Xiaoshi Fanshi powder for the treatment of Heidan disease(black jaundice)is based on the understanding that the nature of Heidan disease(black jaundice)is inseparable from the two key pathological factors of dampness and blood stasis.The treatment of jaundice should be based on removing blood stasis and dampness,supplemented by soothing the liver and promoting the transportation function of spleen,removing blood stasis and harmonizing the collaterals,and promoting diuresis and reducing jaundice.In the treatment of jaundice,removing blood stasis and purging turbidity should be stressed,and powerful tonification should be used with caution.Since blood stasis and turbidity are always intermingling and often complicated with damp heat,the method of warm drying should be used with caution.For promoting blood circulation,removing blood stasis,and dredging the liver-biliarycollaterals,drasticmedicine shouldbeused withcaution.
