[Objective] The aim was to identify the ploidy of sweet clover induced by colchicine. [Method] The sweet clover germinating seeds and colchicine solution were used for materials to research colchicine-induced Melilotu...[Objective] The aim was to identify the ploidy of sweet clover induced by colchicine. [Method] The sweet clover germinating seeds and colchicine solution were used for materials to research colchicine-induced Melilotus. [Result] The results showed that the concentration of 0.2% colchicine solution could induced double sweet clover which showed corresponding features of polyploidy plants as a whole showed great features. [Conclusion] Sweet clover induced by colchicine was polyploidy.展开更多
To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is 'Sligen 1 ", an experiment involving bioactive components detection and field plant...To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is 'Sligen 1 ", an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry (GC-MS) and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.展开更多
To get the IR spectrums of Agrimonia Pilosa Ledeb (APL) from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effect...To get the IR spectrums of Agrimonia Pilosa Ledeb (APL) from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy (FT-IR) was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.展开更多
[Objective] The study aimed to find an efficient and sustainable way to improve the degraded soil quality in the semiarid Loess Plateau. [Method] This study was done with three treatments: (1) the perennial legume ...[Objective] The study aimed to find an efficient and sustainable way to improve the degraded soil quality in the semiarid Loess Plateau. [Method] This study was done with three treatments: (1) the perennial legume species alfalfa (Medicago sativa L.) (AF) planted at a density of 22.5 kg/hm 2 ; (2) the biennial legume species sweetclover (Melilotus officinalis L.) (SF) planted at a density of 11.3 kg/hm 2 ; and (3) natural regeneration (NR). [Result] It is found that NR helped improve deep soil water but with the lowest aboveground biomass. In contrast, AF has the lowest soil water content but with the highest aboveground biomass. Furthermore, in contrast to SF, NR and AF have a higher soil organic carbon and total nitrogen. However, there were no significant differences on soil total and available phosphorus, and soil microbial biomass among all the treatments. As the experiment lasts, AP decreased significantly in all treatments in comparison with their initial values at the beginning of the experiment in 2003. [Conclusion] NR was the best way to restore the deep soil water among all the treatments, and phosphorus fertilizer was necessary for the sustainable development of agricultural production. This research provides a valuable example of soil quality restoration in semiarid regions.展开更多
A simple and accurate high-performance liquid chromatography method (HPLC) was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of th...A simple and accurate high-performance liquid chromatography method (HPLC) was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable ehro- matographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid (H3PO4) as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality ofM. suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.展开更多
基金Supported by General Project of Education Department in Helongjiang (11531263)Key Project of Science and Technology Bureau, Helongjiang Land Reclamation Bureau (HNKXIV-08-06-09)~~
文摘[Objective] The aim was to identify the ploidy of sweet clover induced by colchicine. [Method] The sweet clover germinating seeds and colchicine solution were used for materials to research colchicine-induced Melilotus. [Result] The results showed that the concentration of 0.2% colchicine solution could induced double sweet clover which showed corresponding features of polyploidy plants as a whole showed great features. [Conclusion] Sweet clover induced by colchicine was polyploidy.
文摘To confirm the content and distribution of bioactive components in roots, stems, leaves and grains of Russian Me/i/otus officina/is 'Sligen 1 ", an experiment involving bioactive components detection and field planting was conducted. Kjeldahl method and high performance gas chromatography were used to detect the protein. The automatic amino acid analyzer was used to detect amino acid. Gas chromatography-mass spectrometry (GC-MS) and molecular distillation apparatus were used to confirm the fatty acid content. Gravimetric method was used to detect saponin and alkaloid contents. High performance liquid chromatography was used to detect the coumarin content. The polyphenol content was determined by forint reagent colorimetric method. Anthracene copper-sulfuric acid colorimetry was used to detect the polysaccharide, and aluminum nitrate-sodium nitrite-sodium hydroxide col- orimetric method was used to confirm flavone. The results showed that protein and amino acid were mainly distributed in leaves and seeds. The fatty acid was mainly distributed in seeds. The saponin was mainly distributed in roots and leave. The polyphenol was mainly distributed in roots, stems and leaves. The alkaloid was mainly distributed in stems, leaves and seeds. Seeds mainly contained coumarin. M. officinalis had little polysaccharide and flavone. The total production of bioactive in- gredients was 3 016.3 kg/hm2. The output of crude protein, amino acids and fatty acids was 1 400.6, 1 246.8, 112.2, respectively. The yields of effective components saponins, alkaloids, polyphenols and coumarin were 97.0, 77.9, 41.7, 40.1 kg/hm2, respectively. After separation, extraction and purification, those components can be used as the natural ingredients to develop medicine, food, chemical and other biotechnological products.
基金supported by Research Foundation for Science and Technology Innovation Talents of Harbin(2010RFQXS072)Scientific Research Foundation for the Returned Overseas Chinese Scholars of Heilongjiang Province of China(200923)
文摘To get the IR spectrums of Agrimonia Pilosa Ledeb (APL) from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy (FT-IR) was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.
基金Supported by the Special Fund for Scientific Research in the Public Interest of Ministry of Agriculture(201203030)a grant from the Department of Science and Technology of Anhui Province(1206c0805033)the Special Foundation of the President of Anhui Academy of Agricultural Sciences for Young Scholars(11B1021)~~
文摘[Objective] The study aimed to find an efficient and sustainable way to improve the degraded soil quality in the semiarid Loess Plateau. [Method] This study was done with three treatments: (1) the perennial legume species alfalfa (Medicago sativa L.) (AF) planted at a density of 22.5 kg/hm 2 ; (2) the biennial legume species sweetclover (Melilotus officinalis L.) (SF) planted at a density of 11.3 kg/hm 2 ; and (3) natural regeneration (NR). [Result] It is found that NR helped improve deep soil water but with the lowest aboveground biomass. In contrast, AF has the lowest soil water content but with the highest aboveground biomass. Furthermore, in contrast to SF, NR and AF have a higher soil organic carbon and total nitrogen. However, there were no significant differences on soil total and available phosphorus, and soil microbial biomass among all the treatments. As the experiment lasts, AP decreased significantly in all treatments in comparison with their initial values at the beginning of the experiment in 2003. [Conclusion] NR was the best way to restore the deep soil water among all the treatments, and phosphorus fertilizer was necessary for the sustainable development of agricultural production. This research provides a valuable example of soil quality restoration in semiarid regions.
基金supported by Hubei and Wuhan Engineering Technology Center of Modernization Traditional Chinese Medicine Wuhan Jianmin Pharmacy of TCM Co. Ltd,Wuhan 430054,China.
文摘A simple and accurate high-performance liquid chromatography method (HPLC) was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable ehro- matographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid (H3PO4) as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality ofM. suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.