温带草甸草原土壤N_2O产生过程研究

以大量的室内模拟培养实验,以内蒙古温带草甸草原土壤为研究对象,利用AIM乙炔抑制法,模拟野外条件对原样土壤样品进行N2O产生过程进行研究。实验结果表明:内蒙古温带草甸草原土壤N2O产生过程以硝化作用为主。其中异养硝化作用起主导作用,自养硝化潜势和反硝化潜势在草原植物不同生长季节变化不同,总体上异养硝化潜势>自养硝化潜势>反硝化潜势。由于自养硝化作用在不同季节的发生,使得草甸草原土壤N2O的产生潜势也高、低起伏变化。从而揭示了内蒙古温带草原土壤以异养硝化作用过程为主产生N2O和N2O排放通量较低的微生物学机理。

Vegetation Traits and Soil Properties in Response to Utilization Patterns of Grassland in Hulun Buir City, Inner Mongolia, China

Numerous studies have focused on vegetation traits and soil properties in grassland, few of which concerned about effects of human utilization patterns on grassland yet. Thus, this study hypothesized that human disturbance(e.g., grazing, mowing and fencing) triggered significant variation of biomass partitioning and carbon reallocation. Besides, there existed some differences of species diversity and soil fertility. To address these hypotheses of grassland with diverse utilization patterns in Hulun Buir City, Inner Mongolia, China, we sampled in situ about aboveground biomass(AGB) and belowground biomass(BGB) to evaluate their biomass allocation. Species diversity and soil properties were also investigated. Subsequently, we discussed the relationship of species diversity with environmental conditions, using data collected from 23 sites during the ecological project period of Returning Grazing Lands to Grasslands(RGLG) program. The results were as follows: 1) both AGB and BGB were lower on grazing regime than those on fencing and mowing, but the ratio of root-to-shoot(R/S) was higher on grazing regime than the other two utilization patterns; 2) neither of evenness and Simpson Index was different significantly among all grassland utilization patterns in desert, typical, and meadow grassland at 0.05. In meadow grassland, species richness of fencing pattern was significantly higher than that of grazing pattern(p < 0.05); 3) both of soil organic carbon content and soil available phosphorous content were increased significantly on fencing pattern than grazing pattern(p < 0.05) in desert grassland, and mowing patterns increased the soil nutrients(soil organic carbon, soil total phosphorous, soil available phosphorous, and soil total nitrogen) significantly compared with grazing patterns(p < 0.05) in typical grassland. However, there were no significant differences among utilization patterns in meadow grassland. In conclusion, both of AGB and BGB were increased significantly by fencing. Moreover, species diversity and soil nutrients can be promoted via mowing and fencing. This study suggested that implementation of Ecological Project played a positive role in sustainable grassland utilization of Hulun Buir City and a strong positive influence on the entire temperate grassland.

Diurnal Variation of Soil CO2 Efflux and Its Optimal Measuring Time-window of Temperate Meadow Steppes in Western Songnen Plain, China

In order to study the diurnal variation of soil CO2 effiux from temperate meadow steppes in Northeast China, and determine the best time for observation, a field experiment was conducted with a LI-6400 soil CO2 flux system under five typical plant communi- ties （Suaeda glauca （Sg）, Chloris virgata （Cv）, Puecinellia distans （Pd）, Leymus chinensis （Lc） and Phragmites australis （Pa）） and an alkali-spot land （As） at the meadow steppe of western Songnen Plain. The results showed that the diurnal variation of soil CO2 effiux exhibited a single peak curve in the growing season. Diurnal maximum soil respiration （Rs） often appeared between 1 1：00 and 13：00, while the minimum occurred at 21：00-23：00 or before dawn. Air temperature near the soil surface （Ta） and soil temperature at 10 cm depth （Tlo） exerted dominant control on the diurnal variations of soil respiration. The time-windows 7：00-9：00 could be used as the optimal measuring time to represent the daily mean soil CO2 effiux at the Cv, Pd, Lc and Pa sites. The daily mean soil CO2 effiux was close to the soil COz effiux from 15：00 to 17：00 and the mean of 2 individual soil CO2 effiux from 15：00 to 19：00 at the As and Sg sites, respectively. During nocturnal hours, negative soil CO2 fluxes （CO2 downwards into the soil） were frequently observed at the As and Sg sites, the magnitude of the negative CO2 fluxes were 0.10-1.55 gmol/（m2.s） and 0.10-0.69 gmol/（m2.s）at the two sites. The results im- plied that alkaline soils could absorb CO2 under natural condition, which might have significant implications to the global carbon budget accounting.

Simulated NH_4^+-N Deposition Inhibits CH_4 Uptake and Promotes N_2O Emission in the Meadow Steppe of Inner Mongolia, China

Few studies are conducted to quantify the effects of enhanced N deposition on soil nitrous oxide （N2O） emission and methane （CH4） uptake in the meadow steppe of Inner Mongolia, China. A two-year field experiment was conducted to assess the effects of nitrogen （N） deposition rates （0, 10, and 20 kg N ha-1 year-1 as （NH4）2SO4） on soil N2O and CH4 fluxes. The seasonal and diurnal variations of soil N2O and CH4 fluxes were determined using the static chamber-gas chromatography method during the two growing seasons of 2008 and 2009. Soil temperature, moisture and mineral N （NH4＋-N and NO3-N） concentration were simultaneously measured. Results showed that low level of （NH4）2SO4 （10 kg N ha-1 year-1） did not significantly affect soil CH4 and N20 fluxes and other variables. High level of （NH4）2SO4 （20 kg N ha-1 year-1） significantly increased soil NO3-N concentration by 24.1% to 35.6%, decreased soil CH4 uptake by an average of 20.1%, and significantly promoted soil N2O emission by an average of 98.2%. Soil N2O emission responded more strongly to the added N compared to CH4 uptake. However, soil CH4 fluxes were mainly driven by soil moisture, followed by soil NO3--N concentration. Soil N2O fluxes were mainly driven by soil temperature, followed by soil moisture. Soil inorganic N availability was a key integrator of soil CH4 uptake and N2O emission. These results suggest that the changes of availability of inorganic N induced by the increased N deposition in soil may affect the CH4 and N2O fluxes in the cold semi-arid meadow steppe over the short term.