草莓茎尖组培技术的简化和降低成本试验

现以草莓匍匐茎尖为试材,在草莓组织培养初代接种过程中对茎尖消毒、驯化和培养基配制方面进行简化和廉价替代的试验研究。结果表明:逐层剥离初代接种效果优于常规接种;廉价替代培养基的继代和生根培养与常规培养基本相同;采用简化技术可降低成本55%左右。

现阶段草莓茎尖组织培养防褐化分析

草莓是一种草本植物,生长周期短,果实成熟早,经济效益好。它是中国培育最早、保护最大的水果品种之一。研究草莓茎尖组织培养中防褐变技术的优化措施。

生长抑制剂对草莓茎尖离体保存的影响

试验结果表明,培养基中添加浓度为1.0 mg·L-1的多效唑可保存草莓试管苗至6个月;6.0 mg·L-1脱落酸结合6℃低温处理,可使试管苗保存20个月;2.1%的甘露醇结合6℃低温处理,可使试管苗保存15个月。

草莓无病毒苗培育技术

草莓属蔷薇科、草莓属多年生草本植物，果实为小浆果，营养价值和经济价值较高，除鲜食外，还可制成酱、汁、酒及罐头等多种食品，具有广阔的生产前景。但草莓主要以匍匐茎分株繁殖为主，经长期多代营养繁殖，病毒病日益严重，使植株生长减弱，产量降低，品质变劣，给生产...

Effect of Heat Treatment Combined with Shoot Tip Culture on the Virus-Free of Arena Strawberry

With Arena strawberry as experimental materials, heat treatments combined with shoot tip culture were used to explore their detoxification effects. The results indicated that if not heated, splitting shoot tips directly could not completely remove viruses. On the other hand, it will be obviously improved after shoot tips are heated. Incubators heat treatment （inconstant temperature） with shoot tip culture, constant temperature water bath incubation process with meristem-tip culture, hot air treatment （constant temperature） with apical meristem culture were three methods tested in searching for virus-free of Arena strawberry in this experiment. And their virus-free rates were 78.9%, 86.0% and 50.0% respectively. Of the three methods, the second, constant temperature water bath process with shoot tip culture, with 78.7% survival, is the best. Thus, it can be inferred that constant temperature water bath treatment combined with shoot tip culture might be the most appropriate method to remove viruses of Arena strawberry.