In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The m...In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The method had good linearity,precision,stability and recovery.Chromatographic fingerprints were determined by HPLC-DAD using rosmarinic acid peaks as references,and 17 common peaks were selected.The similarity indexes calculated based on similarity system theory of the 10 samples were higher than 0.948,indicating good correlation among the common peaks.The chromatograms distinguished S.przewalskii from Salvia miltiorrhiza and identified the origins of S.przewalskii.These results suggested that the proposed method was suitable for S.przewalskii quality control.展开更多
基金The Program of Chinese Herbal Medicines Breeding Research of Sichuan(Grant No.2016NYZ0036-3-3)the Program of Sichuan Science and Technology Conditional Platform Construction(Grant No.2018TJPT0013)
文摘In the present study,we developed a novel high-performance liquid chromatography-diode array detection(HPLC-DAD)method for the simultaneous determination and fingerprinting of 15 components in Salvia przewalskii.The method had good linearity,precision,stability and recovery.Chromatographic fingerprints were determined by HPLC-DAD using rosmarinic acid peaks as references,and 17 common peaks were selected.The similarity indexes calculated based on similarity system theory of the 10 samples were higher than 0.948,indicating good correlation among the common peaks.The chromatograms distinguished S.przewalskii from Salvia miltiorrhiza and identified the origins of S.przewalskii.These results suggested that the proposed method was suitable for S.przewalskii quality control.
