The second internal transcribed spacer (ITS2) of Culicoides arakawae was amp lified by PCR, cloned and sequenced. wDNAsis software was used to analyze the IT S2 sequences of C. arakawae , two other Culicoides species ...The second internal transcribed spacer (ITS2) of Culicoides arakawae was amp lified by PCR, cloned and sequenced. wDNAsis software was used to analyze the IT S2 sequences of C. arakawae , two other Culicoides species and three othe r species of mosquitos which were obtained from GenBank. The lengths of the ITS2 region of the three Culicoides species (C.arakawae, C.variipennis and C.imico la) ranged from 205 to 250 bp, while the ITS2 region of Aedes albopictus a nd Anopheles albimanus were 424 bp and 435 bp respectively. The G+C content of C.arakawae was the lowest (20 87%) among the species examined, while tha t of Aedes albopictus (56 35%) and Anopheles albimanus (56 13%) were t he highest. The composition of the ITS2 sequence was highly variable, and there we re no conserved regions with more than two bases in their sequences. The conserv ed loci appeared at positions 9, 113, 117, 134, 141, 164, 197, 212, 292 and 396 loci. After the ITS2 sequences of the three species of Culicoides were align ed, four conserved regions were observed. The highest genetic similarity existed bet ween C.arakawae and C.imicola (40 9%) and the least between C.arakawa e, Aedes albopictus and Anopheles albimanus (7 7%). Since the ITS2 provid es enough genetic markers it could be used as a target region to set up diagnost ic PCR assay for Culicoides .展开更多
以COⅠ、16S r DNA和ITS基因为标记,选用环斑库蠓Culicoides circumscriptus(N267)作为外群,对海峡两岸10个群体77个荒川库蠓标本的遗传距离、基因分化、系统发育和种群结构进行分析。基于COⅠ、16S r DNA基因数据集及ITS基因数据集的AM...以COⅠ、16S r DNA和ITS基因为标记,选用环斑库蠓Culicoides circumscriptus(N267)作为外群,对海峡两岸10个群体77个荒川库蠓标本的遗传距离、基因分化、系统发育和种群结构进行分析。基于COⅠ、16S r DNA基因数据集及ITS基因数据集的AMOVA分析均显示,大的遗传变异主要来自种群内的遗传变异,在线粒体数据集及核糖体数据集的比例分别为96.75%和91.44%。不同基因的分子数据显示,不同种群间的荒川库蠓遗传分化程度低、基因交流频繁,各个地理种群没有按照地理区划形成独特的遗传结构。气流及人为因素可能是造成其远距离扩散的主要原因。展开更多
文摘The second internal transcribed spacer (ITS2) of Culicoides arakawae was amp lified by PCR, cloned and sequenced. wDNAsis software was used to analyze the IT S2 sequences of C. arakawae , two other Culicoides species and three othe r species of mosquitos which were obtained from GenBank. The lengths of the ITS2 region of the three Culicoides species (C.arakawae, C.variipennis and C.imico la) ranged from 205 to 250 bp, while the ITS2 region of Aedes albopictus a nd Anopheles albimanus were 424 bp and 435 bp respectively. The G+C content of C.arakawae was the lowest (20 87%) among the species examined, while tha t of Aedes albopictus (56 35%) and Anopheles albimanus (56 13%) were t he highest. The composition of the ITS2 sequence was highly variable, and there we re no conserved regions with more than two bases in their sequences. The conserv ed loci appeared at positions 9, 113, 117, 134, 141, 164, 197, 212, 292 and 396 loci. After the ITS2 sequences of the three species of Culicoides were align ed, four conserved regions were observed. The highest genetic similarity existed bet ween C.arakawae and C.imicola (40 9%) and the least between C.arakawa e, Aedes albopictus and Anopheles albimanus (7 7%). Since the ITS2 provid es enough genetic markers it could be used as a target region to set up diagnost ic PCR assay for Culicoides .
文摘以COⅠ、16S r DNA和ITS基因为标记,选用环斑库蠓Culicoides circumscriptus(N267)作为外群,对海峡两岸10个群体77个荒川库蠓标本的遗传距离、基因分化、系统发育和种群结构进行分析。基于COⅠ、16S r DNA基因数据集及ITS基因数据集的AMOVA分析均显示,大的遗传变异主要来自种群内的遗传变异,在线粒体数据集及核糖体数据集的比例分别为96.75%和91.44%。不同基因的分子数据显示,不同种群间的荒川库蠓遗传分化程度低、基因交流频繁,各个地理种群没有按照地理区划形成独特的遗传结构。气流及人为因素可能是造成其远距离扩散的主要原因。
