香荔核多糖超声波辅助提取工艺的响应面优化及抗氧化性研究

以香荔核为原料,参照单因素试验结果,以多糖提取率为响应值,采用响应面法优化香荔核多糖的超声波辅助提取工艺,并通过测定香荔核多糖对羟基自由基、超氧阴离子自由基的清除能力及总还原力对其抗氧化活性进行评价。试验表明,超声波辅助热水浸提法提取香荔核多糖的最佳工艺:提取温度58℃,液料比为24∶1(mL/g),超声功率为620 W,超声时间20 min,此条件下,多糖的实测平均提取率为10.04%,与回归模型预测值10.72%相当。当多糖浓度为0.5 mg/mL时,对羟基自由基、超氧阴离子自由基的清除率达到74.44%、86.52%,总还原力为0.596,说明香荔核多糖具有较强的抗氧化能力,且其抗氧化活性与多糖浓度成正向线性关系。

针灸合用自拟荔核丸治疗慢性附睾炎32例

目的:观察针灸配合自拟方药荔核丸治疗慢性附睾炎的疗效。方法:针灸中极、关元、三阴交等穴,自拟方药荔核丸由荔枝核,广桔核,川楝子等13味中药组成。结果:总有效率93.75%。提示:针刺能活血祛瘀,通络止痛;施灸能扶正培元,温通经脉;中药具有软坚散结,活血止痛,清热利湿之功能。综合应用,疗效显著。

基于Wnt/β-catenin信号通路探讨八珍荔核抗纤方对HSC活化增殖、迁移的影响

目的 观察八珍荔核抗纤方对HSC-T6活化增殖、迁移的影响,并探讨其作用机制。方法 将雄性SD大鼠随机分为空白对照组和八珍荔核抗纤方低、中、高剂量组,空白对照组用生理盐水灌胃,含药血清低、中、高组以纯水溶解八珍荔核抗纤方颗粒进行灌胃。换算成SD大鼠的低、中、高剂量分别为7.96g·kg^(-1)、15.93g·kg^(-1)、31.86g·kg^(-1);连续灌胃7d,最后取血制备空白血清和含药血清。通过噻唑蓝(MTT)法测定TGF-β1诱导HSC-T6细胞活化的最佳浓度及不同浓度,八珍荔核抗纤方含药血清对TGF-β1诱导HSC-T6细胞活化增殖影响。细胞划痕愈合法测定八珍荔核抗纤方含药血清对HSC-T6细胞迁移的影响;免疫荧光法测定HSC活化相关指标Col I、α-SMA及Wnt/β-catenin信号通路核心因子β-catenin表达的影响,蛋白质印迹法(western blot)测定八珍荔核抗纤方对HSC活化相关蛋白Col I、α-SMA及Wnt/β-catenin信号通路相关蛋白Wnt1、β-catenin、p-GSK3β、Cyclin D1表达的影响;qPCR测定八珍荔核抗纤方对Wnt/β-catenin信号通路相关因子Wnt1、β-catenin、Cyclin D1表达的影响。结果 MTT实验结果显示,rhTGF-β1刺激HSC-T6活化的最佳浓度为10ng/ml;与模型组比较,八珍荔核抗纤方低、中、高含药血清均能抑制rhTGF-β1刺激的HSC-T6细胞增殖活化(P<0.05),其抑制强度具有剂量依赖性(P<0.05)。划痕实验结果显示,与模型组相比,八珍荔核抗纤方含药血清低、中、高剂量组的细胞迁移能力减弱(P<0.05),且具有剂量依赖性(P<0.05)。Western Blotting实验结果显示,与模型组比较,八珍荔核抗纤方含药血清低、中、高剂量组Col I、α-SMA及β-catenin蛋白表达水平均降低(P<0.05);八珍荔核抗纤方含药血清中、高剂量组Wnt1、p-GSK3β、Cyclin D1蛋白表达水平降低(P<0.05);八珍荔核抗纤方含药血清低剂量组Wnt1、p-GSK3β、Cyclin D1蛋白表达水平无明显变化(P>0.05)。免疫荧光实验结果显示,与模型组比较,八珍荔核抗纤方含药血清低、中、高剂量组Col I、α-SMA及细胞核内β-catenin平均荧光强度均降低(P<0.05)。qPCR实验结果显示,与模型组比较,八珍荔核抗纤方含药血清低、中、高剂量组β-catenin基因表达水平均降低(P<0.05);八珍荔核抗纤方含药血清中、高剂量组Wnt1、Cyclin D1基因表达水平降低(P<0.05);八珍荔核抗纤方含药血清低剂量组Wnt1、Cyclin D1基因表达水平无明显变化(P>0.05)。结论 八珍荔核抗纤方可以抑制HSC的活化增殖、迁移,减少ECM合成进而抗肝纤维化,可能与调控Wnt/β-catenin信号通路有关。

An Effective Method for Extracting Total RNA from Young Embryo of Seedless Litchi

A total RNA extraction method for young embryo of seedless litchi was introduced. CTAB, Phenol （saturated with water）, chloroform, Guanidine isothioeyanate were used as main extraction reagents. Polyphenolie compounds were removed effectively by added PVP into the extraction buffer solution. RNA was purified intensively by phenol, chloroform extraction, and ethanol deposition after deposited by LiCl. Both the results of formaldehyde denatured agarose gel eleetrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA. So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.

中药治疗乳腺增生86例

乳腺增生是妇女常见的一种慢性疾病,中医为" 瘕痞块",临床表现为肿胀,疼痛或能摸到大小不一的肿块.妇女的月经异常与乳腺增生有密切的联系,现代医学都归属于内分泌系统疾病.而中医认为乳腺增生的病理改变与痰凝、气滞血瘀有着密切的关系.

Effects of saponin from the seed of Litchi chinensis Sonn on TGF-β1, FN and SOCS-1 in renal tubular epithelial cells under high glucose

Objective:To investigate the effect of saponin from the seed of Litchi chinensis Sonn(SLS)on the growth and apoptosis of human kidney epithelial cells(HKC)cultured in high glucose.Methods:HKC were cultured in DMEM/F12 medium supplemented with 30 mmol/L glucose and treated with or without SLS.In the normal group,isometric DMEM/F12 medium with 5.5mmol/L glucose was added.The secretion of TGF-β1 and fibronectin(FN)were detected by ELISA.Cell apoptosis was detected by the method of Annexin V-FITC/PI double staining.Western blot was used to detect the level of suppressor of cytokine signaling-1(SOCS-1).Results:The result of ELISA showed that the secretion of TGF-β1 and FN was decreased in SLS groups compared with those in 30 mmol/L glucose treated group(P<0.05).There were more cells apoptosis in 30 mmol/L glucose treated group than that in the normal group(P<0.01).Compared with the 30 mmol/L glucose treated group,the apoptosis of HKC were significantly decreased in SLS groups(P<0.01).Western blot showed that the level of SOCS-1 in high glucose+SLS group was decreased(P<0.01),compared with the high glucose group.Conclusion:SLS can reduce the secretion of TGF-β1 and FN in HKC by reducing the deposition of extracellular matrix.SLS also significantly reduced the apoptosis of HKC by inhibiting the level of SOCS-1.These results suggest the roles of SLS in preventing the progress of glomerular sclerosis.

罗伟生以“化瘀四法”辨治慢性肝病

肝郁是慢性肝病发病的始动因素,脾胃虚弱是慢性肝病发展的根本原因,气虚血瘀,痰瘀阻络是慢性肝病的重要病理过程。辨治慢性肝病要遵循健脾益气、补气生血、补血活血的法则,同时不忘疏肝利胆、利湿祛痰。故基于“因虚致瘀”理论提出化瘀四法,即补气健脾、活血散瘀法适用于慢性肝病之气虚血瘀证;疏肝行气、祛痰通络法适用于气滞血瘀、痰瘀阻络证;养血活血化瘀法适用于慢性肝病之气血不足、瘀血阻络证;软坚消癥化瘀法主要用于重度肝纤维化、肝硬化结节、肝脾肿大、肝内胆汁淤积、肝癌等慢性肝病患者。临证根据慢性肝病的症状体征及病因病机,随症加减药物,创立八珍荔核抗纤方。

罗伟生教授治疗乙型肝炎后肝硬化验案举隅

乙型肝炎后肝硬化是乙型肝炎逐步进展导致的慢性疾病。近年来,越来越多的研究表明,中医治疗乙肝肝硬化具有独特优势。罗伟生教授治疗乙肝肝硬化运用“肝脾同调”的治疗思路,在临床实践中,采用软坚消癥化瘀法,方选自拟方八珍荔核散结方加减治疗乙型肝炎后肝硬化,疗效可观,能够有效改善乙肝肝硬化患者的症状,降低肝功能指标,延缓病情进展。分享罗伟生教授治疗乙型肝炎后肝硬化(肝郁脾虚,气滞血瘀证)医案1则。Post-hepatitis B cirrhosis is a chronic disease caused by the progressive progression of hepatitis B. In recent years, more and more studies have shown that traditional Chinese medicine has unique advantages in the treatment of hepatitis B cirrhosis. Professor Luo Weisheng uses the therapeutic idea of “the same regulation of liver and spleen” in the treatment of hepatitis B cirrhosis. In clinical practice, he uses the method of resolving hard lump and removing blood stasis, and uses the Bazhen Decoction Combined with Lychee-Nut Formula for the treatment of hepatitis B cirrhosis. The curative effect is considerable, which can effectively improve the symptoms of patients with hepatitis B cirrhosis, reduce liver function indexes, and delay the progress of the disease. Professor Luo Weisheng’s medical case of treating post-hepatitis B cirrhosis (liver stagnation and spleen deficiency, qi stagnation and blood stasis syndrome) is shared.