为了减少散射和荧光光子在低能X射线自由空气电离室复现空气比释动能的影响,需要引入散射和荧光光子修正因子。运用EGSnrc MC模拟程序中的DOSXYZnrc程序包对6~50 ke V能量范围内X射线自由空气电离室的散射和荧光光子修正因子进行模拟计...为了减少散射和荧光光子在低能X射线自由空气电离室复现空气比释动能的影响,需要引入散射和荧光光子修正因子。运用EGSnrc MC模拟程序中的DOSXYZnrc程序包对6~50 ke V能量范围内X射线自由空气电离室的散射和荧光光子修正因子进行模拟计算,获得散射和荧光光子修正因子的值;参考CCRI的国际比对规范,对低能X射线规范下的5个辐射质的散射和荧光光子修正因子进行了模拟计算,得出散射和荧光光子修正因子的值。研究结果表明：所得散射和荧光光子修正因子不确定度为0.1%,可为低能X射线空气比释动能的国际比对提供数据支持。展开更多
A new two-photon fluorescence DNA probe,BMVEC,with bigger σ×Φ(1437 GM) was synthesized.The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the gro...A new two-photon fluorescence DNA probe,BMVEC,with bigger σ×Φ(1437 GM) was synthesized.The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the grooves in the double helix of DNA,which enhances its two-photon fluorescent intensity.Meanwhile the double-staining results in cancer cells by means of TPM demonstrate the exclusive nucleic labeling ability of BMVEC,and the positions and amounts of nuclei labeled by BMVEC are wholly the identical as those by DAPI.Moreover,the incident power of BMVEC is lower than that of DAPI.The results indicate that BMVEC can act as the two-photon fluorescent probe of DNA.展开更多
2,7-BHVC,a novel two-photon fluorescent probe for DNA with large two-photon excited fluorescence action cross sections,was synthesized and characterized.It emits weak two-photon fluorescence in aqueous solution while ...2,7-BHVC,a novel two-photon fluorescent probe for DNA with large two-photon excited fluorescence action cross sections,was synthesized and characterized.It emits weak two-photon fluorescence in aqueous solution while its two-photon fluorescence is strongly restored upon binding to DNA.The two-photon imaging in cell demonstrated that 2,7-BHVC can exactly label nuclear DNA.展开更多
文摘为了减少散射和荧光光子在低能X射线自由空气电离室复现空气比释动能的影响,需要引入散射和荧光光子修正因子。运用EGSnrc MC模拟程序中的DOSXYZnrc程序包对6~50 ke V能量范围内X射线自由空气电离室的散射和荧光光子修正因子进行模拟计算,获得散射和荧光光子修正因子的值;参考CCRI的国际比对规范,对低能X射线规范下的5个辐射质的散射和荧光光子修正因子进行了模拟计算,得出散射和荧光光子修正因子的值。研究结果表明：所得散射和荧光光子修正因子不确定度为0.1%,可为低能X射线空气比释动能的国际比对提供数据支持。
基金This work was supported by the National Natural Science Foundation of China(No.21973022)Guangdong Basic and Applied Basic Research Foundation(No.2023A1515012353).
文摘A new two-photon fluorescence DNA probe,BMVEC,with bigger σ×Φ(1437 GM) was synthesized.The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the grooves in the double helix of DNA,which enhances its two-photon fluorescent intensity.Meanwhile the double-staining results in cancer cells by means of TPM demonstrate the exclusive nucleic labeling ability of BMVEC,and the positions and amounts of nuclei labeled by BMVEC are wholly the identical as those by DAPI.Moreover,the incident power of BMVEC is lower than that of DAPI.The results indicate that BMVEC can act as the two-photon fluorescent probe of DNA.
文摘2,7-BHVC,a novel two-photon fluorescent probe for DNA with large two-photon excited fluorescence action cross sections,was synthesized and characterized.It emits weak two-photon fluorescence in aqueous solution while its two-photon fluorescence is strongly restored upon binding to DNA.The two-photon imaging in cell demonstrated that 2,7-BHVC can exactly label nuclear DNA.
