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乙肝病毒免疫标志物与其DNA荧光定量聚合酶链反应之间的关系探讨 被引量:11
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作者 刘庆鸣 曾黎峰 +1 位作者 万本愿 王文丁 《实验与检验医学》 CAS 2001年第5期270-271,共2页
目的探讨乙肝病毒免疫标志物与HBV-DNA之间的相互关系,为临床诊断和治疗提供有价值的判断标准。方法用ELISA法检测血清免疫标志物,并与用荧光定量聚合酶链反应检测HBV-DNA相比较。结果在HBsAg、HBeAg、HBcAb均阳性组,HBsAg、HBeAb、HBcA... 目的探讨乙肝病毒免疫标志物与HBV-DNA之间的相互关系,为临床诊断和治疗提供有价值的判断标准。方法用ELISA法检测血清免疫标志物,并与用荧光定量聚合酶链反应检测HBV-DNA相比较。结果在HBsAg、HBeAg、HBcAb均阳性组,HBsAg、HBeAb、HBcAb均阳性组,HBsAb阳性组,全阴组中,FQ-PCR检出率分别为100%、54.05%、35%和20%。结论提示FQ-PCR可以检测HBV的真实感染和复制状态,结合乙肝病毒的血清标志物检测,对乙肝的临床诊断、治疗方案的选择和疗效观察有较大指导意义。 展开更多
关键词 免疫标志物 ELISA 聚合酶链反应 乙肝病毒 荧光光度测量
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A dual measurement method of strain and temperature
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作者 JIANG Hai-li SUN Wei-min ZHANG Cong LIU Zhi-hai JIANG Fu-qiang ZHANG Yang 《Journal of Marine Science and Application》 2007年第1期63-68,共6页
With the rapid development of China's foreign trade, the coastal and inland waterway transport has been increased rapidly. The potential market for marine engines is more and more obvious. The measurement of the e... With the rapid development of China's foreign trade, the coastal and inland waterway transport has been increased rapidly. The potential market for marine engines is more and more obvious. The measurement of the engine temperature and strain becomes very important. The fluorescence fiber sensors are broadly used to measure temperature, concentration, and pH value, etc. The fluorescence sensing systems are based on different principles, namely fluorescence intensity, fluorescence intensity ratio, and fluorescence lifetime. The fluorescence lifetime is an effective parameter for sensing purpose, because it is independent of the intensity of the pumping source and does not need expensive narrow-band filters. An experiment system has been established, in which some samples were produced to measure the fluorescence lifetime and temperature characteristics and the relationship of the strain and temperature versus the fluorescence lifetime was achieved at the same time. The experiment result was fitted and analyzed. The test results show that the fluorescence lifetime decreases with the increasing of temperature. The change of fluorescence lifetime with the strain is inconspicuous comparing to that with the temperature. 展开更多
关键词 fluorescence lifetime fluorescence fiber simultaneous measurement TEMPERATURE STRAIN
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Optical Fiber Fluorometer for Algae Detection and Mapping
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作者 ZHENGLongjiang DUANXiaoying 《Semiconductor Photonics and Technology》 CAS 1999年第1期35-40,共6页
A simple instrument for the real-time measurement of algae concentration and mapping is described. The instrument uses a pulsed short arc xenon flashlamp as the excited light sources. Both the exciting light and the f... A simple instrument for the real-time measurement of algae concentration and mapping is described. The instrument uses a pulsed short arc xenon flashlamp as the excited light sources. Both the exciting light and the fluorescence from algae chlorophyll are transmitted along a fiber bundle. The measurement sensitivity is analyzed and the experiment result is given. The instrument is practical to in-situ measurement at sea. 展开更多
关键词 Algae Concentration CHLOROPHYLL-A Optical Fiber Fluorometer Real-time Measurement
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