TH742 98042736激光扫描共焦荧光显微镜的电子控制系统研究=Study of electronic control system for laserscanning confocal fluorescence microscope[刊,中]/张平,向际鹰,吴震(华中理工大学光电子工程系.湖北,武汉(430074))∥激光技...TH742 98042736激光扫描共焦荧光显微镜的电子控制系统研究=Study of electronic control system for laserscanning confocal fluorescence microscope[刊,中]/张平,向际鹰,吴震(华中理工大学光电子工程系.湖北,武汉(430074))∥激光技术.—1997,21(5).—284—287介绍了激光扫描共焦荧光显微镜的电子控制系统,展开更多
The phase information of an enlarged source is reconstructed with an annular two-zone phase-only filter in a fluorescent confocal scanning optical microscope for resolution improvement. The dependences of its resoluti...The phase information of an enlarged source is reconstructed with an annular two-zone phase-only filter in a fluorescent confocal scanning optical microscope for resolution improvement. The dependences of its resolution on the source size and on the phase transmission of the outer annular zone of the falter are investigated theoretically by use of its three-dimensional optical transfer function （3D OTF ）. The increased source size and the required phase value of the outer annular zone of the phase-only falter for an optimal 3D OTF of the optical system are presented.展开更多
A novel methodology for imaging wood pulp fibre surface lignin by fluorescence confocal laser scanning microscopy was developed. Various imaging modes and imaging conditions were explored for quantitative analysis. Ac...A novel methodology for imaging wood pulp fibre surface lignin by fluorescence confocal laser scanning microscopy was developed. Various imaging modes and imaging conditions were explored for quantitative analysis. Acridine Orange was used for labelling lignin and the orthochromatic labelling condition was developed. With the thusly established methodology, the distribution of lignin across the fibre wall was clearly imaged. It was found that surface lignin concentration is about 2-4 times higher than bulk lignin concentration, and that high concentration of lignin was also found on the fibre lumen surfaces and pit borders.展开更多
In this paper, the function of the iel gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus （SeMNPV）, belonging to group II nucleopolyhedrovirus, was studied in mammalian cells We amplified the SeMN...In this paper, the function of the iel gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus （SeMNPV）, belonging to group II nucleopolyhedrovirus, was studied in mammalian cells We amplified the SeMNPV iel gene and expressed it by fusing to the C terminal of enhanced GFP protein in HEK 293 cells. Confocal microscopy revealed that the IE1-GFP fusion protein was localized in the nucleus of the mammalian cells. The promoter sequences of AcMNPV gp64, SeMNPV F protein and Drosophila hsp70 were also analyzed, to further study the function of SeMNPV IE1. The results showed that, in the absence of the hr sequence, IE1 improved the expression of the F promoter but didn＇t influence the gp64 promoter significantly, but IE1 moderately stimulated the hsp70 promoter.展开更多
Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three differe...Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different ad- hesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premo- lars and bonded to composite resin using three adhesive resins （FluoroBond II, Xeno-III-Bond, and iBond）. From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid （SBF）. The experimental slabs were immersed in a Portland cement-based remineralization medium that con- tained two biomimetic analogs （biomineralization medium （BRM））. Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance （ANOVA） and Tukey＇s honest significant difference （HSD） tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer （P≤0.05）. A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.展开更多
文摘TH742 98042736激光扫描共焦荧光显微镜的电子控制系统研究=Study of electronic control system for laserscanning confocal fluorescence microscope[刊,中]/张平,向际鹰,吴震(华中理工大学光电子工程系.湖北,武汉(430074))∥激光技术.—1997,21(5).—284—287介绍了激光扫描共焦荧光显微镜的电子控制系统,
文摘The phase information of an enlarged source is reconstructed with an annular two-zone phase-only filter in a fluorescent confocal scanning optical microscope for resolution improvement. The dependences of its resolution on the source size and on the phase transmission of the outer annular zone of the falter are investigated theoretically by use of its three-dimensional optical transfer function （3D OTF ）. The increased source size and the required phase value of the outer annular zone of the phase-only falter for an optimal 3D OTF of the optical system are presented.
文摘A novel methodology for imaging wood pulp fibre surface lignin by fluorescence confocal laser scanning microscopy was developed. Various imaging modes and imaging conditions were explored for quantitative analysis. Acridine Orange was used for labelling lignin and the orthochromatic labelling condition was developed. With the thusly established methodology, the distribution of lignin across the fibre wall was clearly imaged. It was found that surface lignin concentration is about 2-4 times higher than bulk lignin concentration, and that high concentration of lignin was also found on the fibre lumen surfaces and pit borders.
基金The knowledge innovation program of the Chinese Academy of Sciences (KSCX2-YW-Z-0938)
文摘In this paper, the function of the iel gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus （SeMNPV）, belonging to group II nucleopolyhedrovirus, was studied in mammalian cells We amplified the SeMNPV iel gene and expressed it by fusing to the C terminal of enhanced GFP protein in HEK 293 cells. Confocal microscopy revealed that the IE1-GFP fusion protein was localized in the nucleus of the mammalian cells. The promoter sequences of AcMNPV gp64, SeMNPV F protein and Drosophila hsp70 were also analyzed, to further study the function of SeMNPV IE1. The results showed that, in the absence of the hr sequence, IE1 improved the expression of the F promoter but didn＇t influence the gp64 promoter significantly, but IE1 moderately stimulated the hsp70 promoter.
基金Project supported by the National Natural Science Foundation of China(No.81271955)the Zhejiang Provincial Natural Science Foundation of China(No.Y2080338)
文摘Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different ad- hesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premo- lars and bonded to composite resin using three adhesive resins （FluoroBond II, Xeno-III-Bond, and iBond）. From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid （SBF）. The experimental slabs were immersed in a Portland cement-based remineralization medium that con- tained two biomimetic analogs （biomineralization medium （BRM））. Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance （ANOVA） and Tukey＇s honest significant difference （HSD） tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer （P≤0.05）. A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.
