α-N-(S-1-phenylethyl)-β-(4’-methoxyl)benzoyl-L-alanine had been synthesized by combination of Friedal-Craft reaction and stereo-selective Michael reaction.The structure was confirmed by 1H NMR,13C NMR and X-Ray sin...α-N-(S-1-phenylethyl)-β-(4’-methoxyl)benzoyl-L-alanine had been synthesized by combination of Friedal-Craft reaction and stereo-selective Michael reaction.The structure was confirmed by 1H NMR,13C NMR and X-Ray single crystal diffraction.The steady-state fluorescence property of the title compound was also investigated.It was found that the maximum emission wavelength was longer and the quantum yield was less in solvents with larger dielectric constant.The title compound displayed recognition ability to environmental state.展开更多
The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy wa...The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein （EYFP）, the nuclear export signals （NES） of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattem of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 （CRM1）-dependent manner involving RanGTP hydrolysis展开更多
Seven novel fluorescent coumarin derivatives were synthesized from 7-diethylamino-4-chloro-3-formyl-coumarin. The spectra of absorption, excitation and emission were dependent not only on the structures and also on th...Seven novel fluorescent coumarin derivatives were synthesized from 7-diethylamino-4-chloro-3-formyl-coumarin. The spectra of absorption, excitation and emission were dependent not only on the structures and also on the concentration of dyes. The PPP-MO predictions can only be consistent with the spectra in dilute solutions.展开更多
Amino acid neurotransmitters facilitate the transmission of nerve messages across the synapses and play essential roles in the control and regulation of a variety of functions in the central and peripheral nervous sys...Amino acid neurotransmitters facilitate the transmission of nerve messages across the synapses and play essential roles in the control and regulation of a variety of functions in the central and peripheral nervous system. In this study, we developed a sensitive and efficient method using high-performance liquid chromatography （HPLC） with fluorescence detection for the assay of five important amino acid n After derivatization with o-phthaldialdehyde （OPA）, aspartate （Asp）, glutamic acid （Glu）, glycine （Gly）, taurine （Tau） and ）,-aminobutyric acid （GABA） were simultaneously detected in the presence of the internal standard homoserine （Hse）. Precise separation of these five amino acids was achieved using isocratic elution within 24 min. Good linearity was found over the concentration range with correlation coefficients （r2） not less than 0.9998. The limit of detection （LOD） values were no more than 10 nmol/L. The intra- and inter-day reproducibility was adequate with the relative standard deviation （RSD） of 10.5% or below. This method has also been applied to the analysis of amino acids in the substantia nigra and striatum samples obtained from C57BL/6 mice.展开更多
文摘α-N-(S-1-phenylethyl)-β-(4’-methoxyl)benzoyl-L-alanine had been synthesized by combination of Friedal-Craft reaction and stereo-selective Michael reaction.The structure was confirmed by 1H NMR,13C NMR and X-Ray single crystal diffraction.The steady-state fluorescence property of the title compound was also investigated.It was found that the maximum emission wavelength was longer and the quantum yield was less in solvents with larger dielectric constant.The title compound displayed recognition ability to environmental state.
基金the Major State Basic Research Development Program of China(2010CB530105 and 2011CB504802)the National Natural Science Foundation of China(30900059,30870120 and 81000736)the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences(20071010-141)
文摘The function of the herpes simplex virus type 1 （HSV-1） UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein （EYFP）, the nuclear export signals （NES） of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattem of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 （CRM1）-dependent manner involving RanGTP hydrolysis
基金Supported by the State Key Laboratory of Dyes and Surfactants Fine Chemicals.
文摘Seven novel fluorescent coumarin derivatives were synthesized from 7-diethylamino-4-chloro-3-formyl-coumarin. The spectra of absorption, excitation and emission were dependent not only on the structures and also on the concentration of dyes. The PPP-MO predictions can only be consistent with the spectra in dilute solutions.
文摘Amino acid neurotransmitters facilitate the transmission of nerve messages across the synapses and play essential roles in the control and regulation of a variety of functions in the central and peripheral nervous system. In this study, we developed a sensitive and efficient method using high-performance liquid chromatography （HPLC） with fluorescence detection for the assay of five important amino acid n After derivatization with o-phthaldialdehyde （OPA）, aspartate （Asp）, glutamic acid （Glu）, glycine （Gly）, taurine （Tau） and ）,-aminobutyric acid （GABA） were simultaneously detected in the presence of the internal standard homoserine （Hse）. Precise separation of these five amino acids was achieved using isocratic elution within 24 min. Good linearity was found over the concentration range with correlation coefficients （r2） not less than 0.9998. The limit of detection （LOD） values were no more than 10 nmol/L. The intra- and inter-day reproducibility was adequate with the relative standard deviation （RSD） of 10.5% or below. This method has also been applied to the analysis of amino acids in the substantia nigra and striatum samples obtained from C57BL/6 mice.
