We investigate the fluorescence quenching of Rhodamine 6G （R6G）, a well known laser dye with a high fluorescence quantum yield, by as-synthesized graphene oxide （GO） in aqueous solution, which is found to be rathe...We investigate the fluorescence quenching of Rhodamine 6G （R6G）, a well known laser dye with a high fluorescence quantum yield, by as-synthesized graphene oxide （GO） in aqueous solution, which is found to be rather efficient. By means of steady-state and time-resolved fluorescence spectroscopy combined with detailed analysis about the linear absorption vari- ation for this R6G-GO system, the pertinent quenching mechanism has been elucidated to be a combination of dynamic and static quenching. Possible ground-state complexes be- tween R6G and GO during the static quenching have also been suggested. Furthermore, the direction of photoindueed electron transfer between R6G and GO has been discussed.展开更多
The fluorescence quenching of Rhodamine 6G （R6G） by graphene oxide （GO） was interrogated by R6G fluorescence measurements using a set of controlled GO samples with varied C/O ratios as the quencher. The carbonyl g...The fluorescence quenching of Rhodamine 6G （R6G） by graphene oxide （GO） was interrogated by R6G fluorescence measurements using a set of controlled GO samples with varied C/O ratios as the quencher. The carbonyl groups on the GO nanosheet turned to play a dominant role in quenching the R6G fluorescence. The quenching in the static regime can be described by the ＂sphere of action＂ model. The significant absorption of the R6G fluorescence by the ground-state complex formed between R6G and GO was identified to be responsible for the static quenching mechanisms in quenching. This work offers the R6G/GO system. insights into the fluorescence展开更多
The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)fluorescence.The resolved spectra were hi...The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)fluorescence.The resolved spectra were highly similar with the actual ones which indicated that the resolved results were reliable.The relevant parameters of the binding process were obtained by quantifying each substance in the complicated mixtures in situ.The quenching was static quenching,epicatechin had a weak interaction with BSA and the binding site was one.The total concentration and the free concentration of quenchers had different effect on the system.The results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions,avoiding complicated pretreatment and simplify experimental procedure.展开更多
基金This work was supported by the National Basic Research Program of China （No.2010CB923300）, the National Natural Science Foundation of China （No.91127042 and No.21173205）, the Chinese Academy of Sciences （No.XDB01020000）, the FR- FCUC （No.WK2340000012）, the USTC-NSRL Joint Funds （No.KY2340000021）, and the National Under- graduate Innovative Training Program of Ministry of Education （No.201210358064）.
文摘We investigate the fluorescence quenching of Rhodamine 6G （R6G）, a well known laser dye with a high fluorescence quantum yield, by as-synthesized graphene oxide （GO） in aqueous solution, which is found to be rather efficient. By means of steady-state and time-resolved fluorescence spectroscopy combined with detailed analysis about the linear absorption vari- ation for this R6G-GO system, the pertinent quenching mechanism has been elucidated to be a combination of dynamic and static quenching. Possible ground-state complexes be- tween R6G and GO during the static quenching have also been suggested. Furthermore, the direction of photoindueed electron transfer between R6G and GO has been discussed.
基金supported by the Ministry of Science and Technology of China (No.2016YFA0200602)the National Natural Science Foundation of China (No.21573211 and No.21633007)the Fundamental Research Funds for the Central Universities (No.WK2340000063)
文摘The fluorescence quenching of Rhodamine 6G （R6G） by graphene oxide （GO） was interrogated by R6G fluorescence measurements using a set of controlled GO samples with varied C/O ratios as the quencher. The carbonyl groups on the GO nanosheet turned to play a dominant role in quenching the R6G fluorescence. The quenching in the static regime can be described by the ＂sphere of action＂ model. The significant absorption of the R6G fluorescence by the ground-state complex formed between R6G and GO was identified to be responsible for the static quenching mechanisms in quenching. This work offers the R6G/GO system. insights into the fluorescence
基金financially supported by the National Natural Science Foundation of China(21175041)the National Basic Research Program of China(2012CB910602)
文摘The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)fluorescence.The resolved spectra were highly similar with the actual ones which indicated that the resolved results were reliable.The relevant parameters of the binding process were obtained by quantifying each substance in the complicated mixtures in situ.The quenching was static quenching,epicatechin had a weak interaction with BSA and the binding site was one.The total concentration and the free concentration of quenchers had different effect on the system.The results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions,avoiding complicated pretreatment and simplify experimental procedure.
