A novel materials design procedure based on the co-doping of metal nanoparticle and azo dye compound （MNPADC） is developed to improve the properties of functional molecules. The synthesized materials were characteri...A novel materials design procedure based on the co-doping of metal nanoparticle and azo dye compound （MNPADC） is developed to improve the properties of functional molecules. The synthesized materials were characterized by transmission electron micrograph （TEM）, ultraviolet-visible absorption spectra （UV-Vis） and fluorescence spectra （FS）. It was found that the fluorescence intensity of methyl orange （MO） was enhanced by 5 times in the aqueous composite system doped with silver nanoparticles whereas it was reduced by 15% and 20% in composite films with co-mixing and coating structures, respectively. The results indicate that the properties of functional molecules can be greatly improved in composite film with supra molecular structure and that the procedure presented here is effective.展开更多
Biocompatible carbon-spheres-based nanocomposites exhibit great potential in biomedical and clinical applications. In this contribution we report the first green photochemical synthesis of carbon spheres through in-si...Biocompatible carbon-spheres-based nanocomposites exhibit great potential in biomedical and clinical applications. In this contribution we report the first green photochemical synthesis of carbon spheres through in-situ enwrapping around silver nanoparticles(CS–Ag NPs). Since mesoporous carbon spheres can provide the location for combining Ag NPs and other agents, one-step synthesis of glutathione-stabilized CS–Ag NPs could be readily realized by photoreduction. TEM characterization of CS–Ag NPs nanocomposites illustrates that Ag NPs were superbly wrapped inside the carbon spheres and also adhered to the surfaces of the carbon spheres. These porous CS–Ag NPs show excellent fluorescence and effective antibacterial efficiency, exhibiting ideal lengthened activities against Escherichia coli and Staphylococcus aureus compared with bare Ag NPs. The relevant rationale behind it could be attributed to the fact that CS–Ag NPs nanocomposites can provide some excellent niches for the durable and slow release of silver ions. This raises the possibility of promising applications of CS–Ag NPs nanocomposites as excellent antibacterial agents for the efficient monitoring of some disease-related bacteria.展开更多
To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and at- tracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanopa...To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and at- tracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles （AgNPs） were obtained via reduction of Ag＋ on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the tem- plate-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were em- ployed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio （S/B）, the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 μM to 302μM with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%.展开更多
基金This work was supported by the National Natural Science Foundation of China （Grant No. 50271038）the Key Research Project Foundation of Shaanxi Normal University of China （No. 200403） Specialized Research Fund for the Doctoral Program of Higher Education of China （No. 20050698017）.
文摘A novel materials design procedure based on the co-doping of metal nanoparticle and azo dye compound （MNPADC） is developed to improve the properties of functional molecules. The synthesized materials were characterized by transmission electron micrograph （TEM）, ultraviolet-visible absorption spectra （UV-Vis） and fluorescence spectra （FS）. It was found that the fluorescence intensity of methyl orange （MO） was enhanced by 5 times in the aqueous composite system doped with silver nanoparticles whereas it was reduced by 15% and 20% in composite films with co-mixing and coating structures, respectively. The results indicate that the properties of functional molecules can be greatly improved in composite film with supra molecular structure and that the procedure presented here is effective.
基金supported by the National Basic Research Program of China(2010CB732404)the National Natural Science Foundation of China(81325011,21327902,21175020)+1 种基金the National High Technology Research and Development Program of China(2012AA022703)the Suzhou Science&Technology Major Project(ZXY2012028)
文摘Biocompatible carbon-spheres-based nanocomposites exhibit great potential in biomedical and clinical applications. In this contribution we report the first green photochemical synthesis of carbon spheres through in-situ enwrapping around silver nanoparticles(CS–Ag NPs). Since mesoporous carbon spheres can provide the location for combining Ag NPs and other agents, one-step synthesis of glutathione-stabilized CS–Ag NPs could be readily realized by photoreduction. TEM characterization of CS–Ag NPs nanocomposites illustrates that Ag NPs were superbly wrapped inside the carbon spheres and also adhered to the surfaces of the carbon spheres. These porous CS–Ag NPs show excellent fluorescence and effective antibacterial efficiency, exhibiting ideal lengthened activities against Escherichia coli and Staphylococcus aureus compared with bare Ag NPs. The relevant rationale behind it could be attributed to the fact that CS–Ag NPs nanocomposites can provide some excellent niches for the durable and slow release of silver ions. This raises the possibility of promising applications of CS–Ag NPs nanocomposites as excellent antibacterial agents for the efficient monitoring of some disease-related bacteria.
基金supported by the National Natural Foundation of China (21075032 & 21005026)National Key Basic Research Program (2011CB911000)Hunan Province Key Project of Scientific & Tech-nical Programs (2010TP4013-1)
文摘To develop the high-performance fluorescent bio-sensors, the metal nanoparticles were employed as nanoquenchers and at- tracted reasonable attention in the design of fluorescent biosensors. In this work, silver nanoparticles （AgNPs） were obtained via reduction of Ag＋ on FAM-labeled DNA template. For the tight binding between AgNPs and DNA, the tem- plate-synthesized AgNPs turned out high quenching efficiency and could be applied as super nanoquenchers to establish the biosensing platform for fluorescent detection. As an example, the template-synthesized DNA-AgNPs conjugates were em- ployed in sensing thiols. By forming S-Ag bonds, thiols interact intensely with AgNPs and replace the FAM-labeled DNA off from the surface of AgNPs, resulting in a fluorescence enhancement. Besides the advantages of lower background and higher signal-to-background ratio （S/B）, the conjugates present better stability, making them applicable in complicated biological fluids. To further evidence the feasibility of sensing thiols in real samples, the thiols in human urine were detected. The total amount of free thiols found in human urine was ranging from 229 μM to 302μM with the proposed sensor. To conclude the reliability, low content of Cys was added and the recovery was 98%-103%.
