Background: Psoriasis is a common inflammatory skin disease characterized by uncontrolled proliferation of keratinocytes and recruitment of T lymphocytes int o the skin. The possible role of human endogenous retroviru...Background: Psoriasis is a common inflammatory skin disease characterized by uncontrolled proliferation of keratinocytes and recruitment of T lymphocytes int o the skin. The possible role of human endogenous retroviruses (HERVs) in the in duction of psoriasis has been suggested, based upon the previous observations of retrovirus-like particles in psoriasis from skin lesional plaques, urine and stimulated lymphocytes. Objectives: To investigate the expression of HERV-E tr ansmembrane envelope glycoprotein (HERV-E env) in normal, psoriatic and atopic human skin, and to examine the influence of ultraviolet (UV) B irradiation onHE RV-E env expression in normal human epidermal keratinocytes. Methods: The anal ysis was performed on both skin biopsies and organotypic skin cultures using imm unofluorescence andWestern immunoblotting. UVB irradiation (312 nm) of cultured normal human keratinocytes was performed using a dose of 30 mJ cm- 2. Results: Positive staining was observed in most of the psoriatic and atopic skin samples, whereas only 15% of the normal skin samples were faintly positive. In additio n, the pattern of expression of HERV- E env differed markedly in psoriasis vs. atopy. By Western blotting analysis, two main proteins of 54 and 57 kDa were det ected in extracts of normal skin, normal keratinocyte cultures and reconstructed epidermis from psoriatic and normal punch biopsies. An increased level of expre ssion of these proteins was noted in extracts from psoriatic vs. normal reconstr ucted epidermis. The overexpression of the 57- kDa protein in normal human cult ured keratinocytes was dramatically reduced by UVB irradiation. Conclusions: The se data suggest for the first time that HERV-E env is expressed in normal and pathological human skin. Further studies are now required to elucidate the role of such viral proteins in the pathogenesis of psoriasis.展开更多
目的分析免疫荧光分析法诊断A、B型流感病毒中的临床应用情况。方法研究时段自2016年7月-2018年7月,选定本院100例流感患者鼻咽拭子样本,采用GICA(胶体金免疫层析)法以及Sofia InfluenzaA+B FIA检测系统检测A、B型流感病毒。结果 Sofia ...目的分析免疫荧光分析法诊断A、B型流感病毒中的临床应用情况。方法研究时段自2016年7月-2018年7月,选定本院100例流感患者鼻咽拭子样本,采用GICA(胶体金免疫层析)法以及Sofia InfluenzaA+B FIA检测系统检测A、B型流感病毒。结果 Sofia InfluenzaA+B FIA检测法的阳性检出率(95.00%)显著高于GICA检测法(62.00%)的,差异具有统计学意义(P<0.05)。结论 Sofia InfluenzaA+B FIA检测系统具有较高的特异性、灵敏度,可迅速诊断A、B型流感病毒,值得借鉴。展开更多
文摘Background: Psoriasis is a common inflammatory skin disease characterized by uncontrolled proliferation of keratinocytes and recruitment of T lymphocytes int o the skin. The possible role of human endogenous retroviruses (HERVs) in the in duction of psoriasis has been suggested, based upon the previous observations of retrovirus-like particles in psoriasis from skin lesional plaques, urine and stimulated lymphocytes. Objectives: To investigate the expression of HERV-E tr ansmembrane envelope glycoprotein (HERV-E env) in normal, psoriatic and atopic human skin, and to examine the influence of ultraviolet (UV) B irradiation onHE RV-E env expression in normal human epidermal keratinocytes. Methods: The anal ysis was performed on both skin biopsies and organotypic skin cultures using imm unofluorescence andWestern immunoblotting. UVB irradiation (312 nm) of cultured normal human keratinocytes was performed using a dose of 30 mJ cm- 2. Results: Positive staining was observed in most of the psoriatic and atopic skin samples, whereas only 15% of the normal skin samples were faintly positive. In additio n, the pattern of expression of HERV- E env differed markedly in psoriasis vs. atopy. By Western blotting analysis, two main proteins of 54 and 57 kDa were det ected in extracts of normal skin, normal keratinocyte cultures and reconstructed epidermis from psoriatic and normal punch biopsies. An increased level of expre ssion of these proteins was noted in extracts from psoriatic vs. normal reconstr ucted epidermis. The overexpression of the 57- kDa protein in normal human cult ured keratinocytes was dramatically reduced by UVB irradiation. Conclusions: The se data suggest for the first time that HERV-E env is expressed in normal and pathological human skin. Further studies are now required to elucidate the role of such viral proteins in the pathogenesis of psoriasis.
文摘目的分析免疫荧光分析法诊断A、B型流感病毒中的临床应用情况。方法研究时段自2016年7月-2018年7月,选定本院100例流感患者鼻咽拭子样本,采用GICA(胶体金免疫层析)法以及Sofia InfluenzaA+B FIA检测系统检测A、B型流感病毒。结果 Sofia InfluenzaA+B FIA检测法的阳性检出率(95.00%)显著高于GICA检测法(62.00%)的,差异具有统计学意义(P<0.05)。结论 Sofia InfluenzaA+B FIA检测系统具有较高的特异性、灵敏度,可迅速诊断A、B型流感病毒,值得借鉴。
