[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown ch...[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown chickens were randomly divided into six groups, including three control groups （normal temperature control group, high temperature control group, high temperature Vc control group） and three high-temperature administration groups （high-dose administration group, moderate-dose administration group, low-dose administration group）. Chickens in normal temperature control group were reared at 14-25 ℃, and those in other five groups were reared at 28-39 ℃. The experiment lasted 10 d. Five chickens in each group were euthanized at 1, 4, 8 and 10 d post-treatment, respectively. The duodenal mucosa was collected and prepared into tissue slices with the conventional method for hematoxylin-eosin （HE） staining. Mucosal thickness, villus length and crypt depth of duodenal mucosa were measured. [Result] Under heat stress, duodenal mucosal thickness and villus length were both significantly lower than those in normal temperature control group, and the duodenal villi were scarce and thin. However, under high temperature conditions, various indicators in high-dose and moderate-dose administration groups were higher than those in high temperature control group and high temperature Vc control group, which exhibited extremely significant differences at 8 and 10 d post-treatment, especially. Moreover, various indicators in high-dose administration group were significantly higher than those in moderate-dose and low-dose administration groups. The traditional Chinese herbal medicine prescription exhibited higher protective effects on duodenal mucosa of chickens under heat stress compared with high temperature Vc control group. [Conclusion] Under heat stress, traditional Chinese herbal medicine reduced effectively the duodenal mucosa damage in chickens.展开更多
Uniformity of warhead axial charge and influences of different warhead wall thicknesses on measurement results were studied by industrial computed tomography(C T).By comparing the differences of relative density value...Uniformity of warhead axial charge and influences of different warhead wall thicknesses on measurement results were studied by industrial computed tomography(C T).By comparing the differences of relative density values of the same simulation charge sample assembled in simulation bodies with different wall thicknesses,effects of warhead wall thickness on charge CT relative density values were analyzed.The results show that CT value increases by about1%with the increase of each additional1mm of wall thickness under the same simulation charge for the projectile with outer diameter of100mm and internal diameterof90mm.There fore,to detect uniformity along warhead axial(upper,middle and lower sections)charge density within penetration ability range of industrial CT(IC T),the CT values of various parts(upper,middle and lower sections)may be only measured without measuring absolute density of charge.By subtracting changes in the CT values caused by warhead wall thickness variation,the CT values of various parts under the same charge can describe warhead axial charge uniformity.展开更多
Horizontal section top-coal(HSTCC)caving offers a powerful method to efficiently excavate rude coal in steep and thick seams,and pre-blasting weakening has a profound effect on pursuing great production,high efficienc...Horizontal section top-coal(HSTCC)caving offers a powerful method to efficiently excavate rude coal in steep and thick seams,and pre-blasting weakening has a profound effect on pursuing great production,high efficiency and good benefit under particular conditions like a small-scale working face with large-scale sectional caving height.+564-level HSTCC working face in B3–6coal seams of Jiangou Colliery in Urumqi was taken as study case for in situ industrial experiment.Total thickness of seams in the study case is about 50.0 m and average angel here is over 83°.In the industrial experiments,at first we adopted continuous charge machine and emulsion matrix explosive to substitute for traditional blasting schemes for specific geological settings in the study case.Hybrid analyses and assessments with blasting crack propagation analysis,abutment pressure monitoring prediction and economical benefit assessment were attributed to be able to attest pre-blasting weakening effects practically.Meanwhile crack propagation analysis after pre-blasting weakening showed that in all triple monitoring bore holes rock masses of top-coal would be fallen into three stages from the bottom up:fracture zone,plastic zone and elastic zone generally,and fracture toughness respectively in correspondent zones was calculated by the analytical formula:0.5616–0.8806,0.6403–0.9541 and0.7535–1.1900 MPa m1/2after pre-blasting weakening.Pressure monitoring prediction and economical benefit assessment also indicated that it was necessary to introduce the pre-blasting weakening with predominant blasting scheme from both views.For excavation in extremely steep and thick coal seams,relevant results would be a useful tool to study the mechanism of pre-blasting weakening both qualitatively and quantitatively.展开更多
High-performance liquid chromatography （HPLC） was used to quantify magnaldehyde B （6）, magnaldehyde E （4） and 8＇,9＇-dihydroxyhonokiol （7） simultaneously in the raw Chinese medicinal material honokiol. The se...High-performance liquid chromatography （HPLC） was used to quantify magnaldehyde B （6）, magnaldehyde E （4） and 8＇,9＇-dihydroxyhonokiol （7） simultaneously in the raw Chinese medicinal material honokiol. The separation was performed on a reversed-phase Cl8 column by using a gradient elution with mobile phases of water （A） and methanol （B）. The mobile phase gradient was run from 40% B to 56.5% B in 55 min, 55-67 rain from 56.5% to 51.5%, 67-80 min from 51.5% to 70%, 80-170 min at 70%. The elution was carried out at a flow rate of 1.0 mL/min at the column temperature of 35 ~C with the UV detection wavelength at 256 am. Magnaldehyde B, magnaldehyde E and 8＇,9＇-dihydroxyhonokiol showed good linear relationships with peak areas in the range of 0.00864 to 0.07776 mg/mL, 0.01488 to 0.13392 mg/mL and 0.01568 to 0.10976 mg/mL, respectively. Their corresponding average recoveries were 100.30%, 99.63% and 98.29%, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility for evaluating the quality of raw Chinese medicinal material honokiol. Moreover, another five phenolic compounds, namely erythro-7-O-methylhonoldtriol （1）, threo-7-O-methylhonokitriol （2）, 7-O-ethylhonokitriol （3）, magnaldehyde C （5）, honokiol （8）, together with compounds 4, 6 and 7, were isolated and purified from the remaining substance in the process of preparing the raw material honokiol by silica gel column and semi-preparative HPLC. Their structures were characterized by ID and 2D NMR spectroscopy. Among them, compounds 1 and 2 were reported to have common planar structures and their relative configurations were identified for the first time. Compounds 3 and 7 were not only obtained from the raw medicinal material for the first time but also novel compounds.展开更多
Traditional Chinese herbal drugs have been used for thousands of years in Chinese pharmacopoeia. The bark of Magnolia officinalis Rehder ＆ E. Wilson, known under the pinyin name ＂Houpo＂, has been traditionally used...Traditional Chinese herbal drugs have been used for thousands of years in Chinese pharmacopoeia. The bark of Magnolia officinalis Rehder ＆ E. Wilson, known under the pinyin name ＂Houpo＂, has been traditionally used in Chinese and Japanese medicines for the treatment of anxiety, asthma, depression, gastrointestinal disorders, headache, and more. Moreover, Magnolia bark extract is a major constituent of currently marketed dietary supplements and cosmetic products. Much pharmacological activity has been reported for this herb and its major compounds notably antioxidant, anti-inflammatory, antibiotic and antispasmodic effects. However, the mechanisms underlying this have not been elucidated and only a very few clinical trials have been published. In vitro and in vivo toxicity studies have also been published and indicate some intriguing features. The present review aims to summarize the literature on M. officinalis bark composition, utilisation, pharmacology, and safety.展开更多
The key to improve the therapeutic efficacy for cancer treatment is to increase the delivery of drugs to tumors.For this purpose, tumor-microenvironment stimuliresponsive materials have great potential. Here, we prepa...The key to improve the therapeutic efficacy for cancer treatment is to increase the delivery of drugs to tumors.For this purpose, tumor-microenvironment stimuliresponsive materials have great potential. Here, we prepared a new nanomedicine by bonding the conjugate of honokiol(HNK) and 5,6-dimethylxanthenone-4-acetic acid(DMXAA)to a glutathione(GSH)-responsive nanocarrier, poly(α-lipoic acid) polyethylene glycol. The nanomedicine would disintegrate due to the high level of GSH at the tumor sites,achieving the co-delivery of HNK and DMXAA, and realizing the combination therapy through close-range killing by HNK and long-range striking by DMXAA together. In a murine 4T1 breast tumor model, this strategy exhibited high tumor inhibition rate of 93%, and provided a valuable therapeutic choice for cancer therapy.展开更多
基金Supported by Agricultural Science and Technology Achievement Transformation Project of the Ministry of Science and Technology(2012GB2A200044)Project of Science and Technology Department of Hebei Province(13826615D)~~
文摘[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown chickens were randomly divided into six groups, including three control groups （normal temperature control group, high temperature control group, high temperature Vc control group） and three high-temperature administration groups （high-dose administration group, moderate-dose administration group, low-dose administration group）. Chickens in normal temperature control group were reared at 14-25 ℃, and those in other five groups were reared at 28-39 ℃. The experiment lasted 10 d. Five chickens in each group were euthanized at 1, 4, 8 and 10 d post-treatment, respectively. The duodenal mucosa was collected and prepared into tissue slices with the conventional method for hematoxylin-eosin （HE） staining. Mucosal thickness, villus length and crypt depth of duodenal mucosa were measured. [Result] Under heat stress, duodenal mucosal thickness and villus length were both significantly lower than those in normal temperature control group, and the duodenal villi were scarce and thin. However, under high temperature conditions, various indicators in high-dose and moderate-dose administration groups were higher than those in high temperature control group and high temperature Vc control group, which exhibited extremely significant differences at 8 and 10 d post-treatment, especially. Moreover, various indicators in high-dose administration group were significantly higher than those in moderate-dose and low-dose administration groups. The traditional Chinese herbal medicine prescription exhibited higher protective effects on duodenal mucosa of chickens under heat stress compared with high temperature Vc control group. [Conclusion] Under heat stress, traditional Chinese herbal medicine reduced effectively the duodenal mucosa damage in chickens.
文摘Uniformity of warhead axial charge and influences of different warhead wall thicknesses on measurement results were studied by industrial computed tomography(C T).By comparing the differences of relative density values of the same simulation charge sample assembled in simulation bodies with different wall thicknesses,effects of warhead wall thickness on charge CT relative density values were analyzed.The results show that CT value increases by about1%with the increase of each additional1mm of wall thickness under the same simulation charge for the projectile with outer diameter of100mm and internal diameterof90mm.There fore,to detect uniformity along warhead axial(upper,middle and lower sections)charge density within penetration ability range of industrial CT(IC T),the CT values of various parts(upper,middle and lower sections)may be only measured without measuring absolute density of charge.By subtracting changes in the CT values caused by warhead wall thickness variation,the CT values of various parts under the same charge can describe warhead axial charge uniformity.
基金provided by the National Natural Science Foundation of China (No.11002021)the Doctoral Subject,Foundation of the Ministry of Education of China (No.20070008012)+1 种基金the National High Technology Research and Development Program of China (No.2008AA062104)Team Program for Key Scientific and Technological innovation,Shaanxi Province of China (No.2013KCT-16)
文摘Horizontal section top-coal(HSTCC)caving offers a powerful method to efficiently excavate rude coal in steep and thick seams,and pre-blasting weakening has a profound effect on pursuing great production,high efficiency and good benefit under particular conditions like a small-scale working face with large-scale sectional caving height.+564-level HSTCC working face in B3–6coal seams of Jiangou Colliery in Urumqi was taken as study case for in situ industrial experiment.Total thickness of seams in the study case is about 50.0 m and average angel here is over 83°.In the industrial experiments,at first we adopted continuous charge machine and emulsion matrix explosive to substitute for traditional blasting schemes for specific geological settings in the study case.Hybrid analyses and assessments with blasting crack propagation analysis,abutment pressure monitoring prediction and economical benefit assessment were attributed to be able to attest pre-blasting weakening effects practically.Meanwhile crack propagation analysis after pre-blasting weakening showed that in all triple monitoring bore holes rock masses of top-coal would be fallen into three stages from the bottom up:fracture zone,plastic zone and elastic zone generally,and fracture toughness respectively in correspondent zones was calculated by the analytical formula:0.5616–0.8806,0.6403–0.9541 and0.7535–1.1900 MPa m1/2after pre-blasting weakening.Pressure monitoring prediction and economical benefit assessment also indicated that it was necessary to introduce the pre-blasting weakening with predominant blasting scheme from both views.For excavation in extremely steep and thick coal seams,relevant results would be a useful tool to study the mechanism of pre-blasting weakening both qualitatively and quantitatively.
基金Major Projects of National Science and Technology on New Drug Creation and Development(Grant No.2009ZX09102-146)
文摘High-performance liquid chromatography （HPLC） was used to quantify magnaldehyde B （6）, magnaldehyde E （4） and 8＇,9＇-dihydroxyhonokiol （7） simultaneously in the raw Chinese medicinal material honokiol. The separation was performed on a reversed-phase Cl8 column by using a gradient elution with mobile phases of water （A） and methanol （B）. The mobile phase gradient was run from 40% B to 56.5% B in 55 min, 55-67 rain from 56.5% to 51.5%, 67-80 min from 51.5% to 70%, 80-170 min at 70%. The elution was carried out at a flow rate of 1.0 mL/min at the column temperature of 35 ~C with the UV detection wavelength at 256 am. Magnaldehyde B, magnaldehyde E and 8＇,9＇-dihydroxyhonokiol showed good linear relationships with peak areas in the range of 0.00864 to 0.07776 mg/mL, 0.01488 to 0.13392 mg/mL and 0.01568 to 0.10976 mg/mL, respectively. Their corresponding average recoveries were 100.30%, 99.63% and 98.29%, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility for evaluating the quality of raw Chinese medicinal material honokiol. Moreover, another five phenolic compounds, namely erythro-7-O-methylhonoldtriol （1）, threo-7-O-methylhonokitriol （2）, 7-O-ethylhonokitriol （3）, magnaldehyde C （5）, honokiol （8）, together with compounds 4, 6 and 7, were isolated and purified from the remaining substance in the process of preparing the raw material honokiol by silica gel column and semi-preparative HPLC. Their structures were characterized by ID and 2D NMR spectroscopy. Among them, compounds 1 and 2 were reported to have common planar structures and their relative configurations were identified for the first time. Compounds 3 and 7 were not only obtained from the raw medicinal material for the first time but also novel compounds.
文摘Traditional Chinese herbal drugs have been used for thousands of years in Chinese pharmacopoeia. The bark of Magnolia officinalis Rehder ＆ E. Wilson, known under the pinyin name ＂Houpo＂, has been traditionally used in Chinese and Japanese medicines for the treatment of anxiety, asthma, depression, gastrointestinal disorders, headache, and more. Moreover, Magnolia bark extract is a major constituent of currently marketed dietary supplements and cosmetic products. Much pharmacological activity has been reported for this herb and its major compounds notably antioxidant, anti-inflammatory, antibiotic and antispasmodic effects. However, the mechanisms underlying this have not been elucidated and only a very few clinical trials have been published. In vitro and in vivo toxicity studies have also been published and indicate some intriguing features. The present review aims to summarize the literature on M. officinalis bark composition, utilisation, pharmacology, and safety.
基金supported by the Ministry of Science and Technology of China (2018ZX09711003-012)the National Natural Science Foundation of China (51873206, 51673189, 51829302, 51503202, 51833010 and 51520105004)the Program of Scientific Development of Jilin Province (20190103033JH)
文摘The key to improve the therapeutic efficacy for cancer treatment is to increase the delivery of drugs to tumors.For this purpose, tumor-microenvironment stimuliresponsive materials have great potential. Here, we prepared a new nanomedicine by bonding the conjugate of honokiol(HNK) and 5,6-dimethylxanthenone-4-acetic acid(DMXAA)to a glutathione(GSH)-responsive nanocarrier, poly(α-lipoic acid) polyethylene glycol. The nanomedicine would disintegrate due to the high level of GSH at the tumor sites,achieving the co-delivery of HNK and DMXAA, and realizing the combination therapy through close-range killing by HNK and long-range striking by DMXAA together. In a murine 4T1 breast tumor model, this strategy exhibited high tumor inhibition rate of 93%, and provided a valuable therapeutic choice for cancer therapy.
