The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe＇s milk and consequently to draw conclusions concerning the health condition of the ...The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe＇s milk and consequently to draw conclusions concerning the health condition of the animal flocks and the current trends of antibiotic use in small ruminants. A total of 77 mountainous sheepfolds were investigated representing 10% of all transhumant sheep and goats flocks in the area. Duplicate milk samples were collected from January to February transferred to the laboratory and analyzed for the presence of Streptococcus spp. by using the serial dilution method and plating on the surface of Slanetz and Bartley agar. The isolates were tested against nine antimicrobial agents with the Kirby-Bauer disk diffusion method. The results showed that Streptococcus spp. occurred in 84.4% of the samples and in counts ranged between 1.8 log CFU/mL and 4.6 log CFU/mL. Concerning susceptibility, only 1.3% of the isolates were resistant to all antimicrobials while 27.3% were fully susceptible. Resistance to cefepime was the most common （93.1%） observation followed by resistance to ampicillin （82.1%）, ciprofloxacine （58.3%） and meropenem （51.9%）. The isolates were vancomycin susceptible at a rate of 83.6%. Multidrug resistance to at least three antibiotics was observed to be 22.1% of Streptococcus spp.. Given the prevalence of Streptococcus spp. and the elevated resistance of isolates to various antibiotics it is concluded that further examinations and even interventions are required in order to pertain the appropriate animal health and milk quality.展开更多
We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli （type I） has been exposed to the dipolar magnetic field force （400, 800, 1200 and 1600 Gausses） which pr...We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli （type I） has been exposed to the dipolar magnetic field force （400, 800, 1200 and 1600 Gausses） which prepared locally with incubation for different period times （24, 48 and 72 hrs） at 37℃. The effects were evaluated by optical density （OD） at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard （0.5）, in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups.展开更多
文摘The purpose of this study was to determine the occurrence and antimicrobial resistance of Streptococcus spp. isolated from fresh ewe＇s milk and consequently to draw conclusions concerning the health condition of the animal flocks and the current trends of antibiotic use in small ruminants. A total of 77 mountainous sheepfolds were investigated representing 10% of all transhumant sheep and goats flocks in the area. Duplicate milk samples were collected from January to February transferred to the laboratory and analyzed for the presence of Streptococcus spp. by using the serial dilution method and plating on the surface of Slanetz and Bartley agar. The isolates were tested against nine antimicrobial agents with the Kirby-Bauer disk diffusion method. The results showed that Streptococcus spp. occurred in 84.4% of the samples and in counts ranged between 1.8 log CFU/mL and 4.6 log CFU/mL. Concerning susceptibility, only 1.3% of the isolates were resistant to all antimicrobials while 27.3% were fully susceptible. Resistance to cefepime was the most common （93.1%） observation followed by resistance to ampicillin （82.1%）, ciprofloxacine （58.3%） and meropenem （51.9%）. The isolates were vancomycin susceptible at a rate of 83.6%. Multidrug resistance to at least three antibiotics was observed to be 22.1% of Streptococcus spp.. Given the prevalence of Streptococcus spp. and the elevated resistance of isolates to various antibiotics it is concluded that further examinations and even interventions are required in order to pertain the appropriate animal health and milk quality.
文摘We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli （type I） has been exposed to the dipolar magnetic field force （400, 800, 1200 and 1600 Gausses） which prepared locally with incubation for different period times （24, 48 and 72 hrs） at 37℃. The effects were evaluated by optical density （OD） at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard （0.5）, in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups.
