岳阳地区328份阴道分泌物标本真菌培养及药敏分析

[目的]了解岳阳地区女性生殖道真菌感染率和选用有效抗真菌药物。[方法]用328份2006年岳阳市妇幼保健院妇科病人的阴道分泌物(白带)标本进行真菌培养和药敏试验。[结果]328份白带标本中共培养出真菌112株,真菌感染率为34.2%,其中白色念珠菌感染率为41%;在药敏试验所选7种抗真菌药物中二性霉素B和制霉菌素仍具有较高的抗菌活性。[结论]真菌引起的女性生殖道感染有逐年增高的趋势,白色念珠菌仍是引起女性生殖道感染的主要真菌;真菌对大多数抗真菌药物具有不同程度的耐药性,但对二性霉素B和制霉菌素的耐药率最低。

女性泌尿生殖道感染患者支原体培养及药敏分析

目的了解女性泌尿生殖道感染患者解脲(Uu)和人型支原体(Mh)感染及耐药情况。方法采用支原体培养、鉴定、药敏一体化试剂盒对825例女性泌尿生殖道感染患者进行支原体培养及药敏试验。结果825例患者中检测出支原体463例,总感染率为56.1%。其中Uu阳性389例(47.1%),Mh阳性26例(3.2%),Uu+Mh混合感染30例(5.8%)。支原体对10抗生素敏感性最高的是强力霉素、美满霉素(92%、86.8%),最低的是壮观霉素(14.7%)。结论女性泌尿生殖道感染患者支原体感染率明显增加,支原体在体外对多种抗菌药物耐药现象严重,治疗宜宜选强力霉素或美满霉素。

Empirical Study on Chemotherapeutic Susceptibility of SP Cells in Human Pulmonary Adenocarcinoma Cell Line A549 in Vitro

OBJECTIVE To explore the chemotherapeutic susceptibility of SP cells in human pulmonary adenocarcinoma cell line A549 and the possible mechanism of multidrug resistance.METHODS SP and non-SP （NSP） cells in the cell line A549 were isolated by fluorescence activated cell sorter. The susceptibility of SP and NSP cells to DDP, 5-FU, VP16, NVB and GEM was detected by a drug susceptibility test, and IC50s were calculated 24 h a er the chemotherapy; and then a er a 2-hour IC50 treatment with 5 chemotherapeutic drugs on the 2 subsets of NSP cells, the intracellular drug levels were determined and analyzed using high performance liquid chromatograph.RESULTS There was no statistical signifi cance in comparison of the di. erences in IC50s and in intracellular drug levels a er DDP treatment between the 2 subsets （P 〉 0.05）, （P 〉 0.05）. However,all IC50s of the other 4 drugs were significantly higher in the SP cells than in the NSP cells （P 〈 0.01）. A er the chemotherapy, the intracellular drug levels of the other 4 drugs were significantly lower in SP cells than in NSP cells （P 〈 0.01）.CONCLUSION Compared to NSP cells, SP cells in the cell line A549 have stronger resistance to the chemotherapeutics. The multidrug resistance of SP cells closely correlates with the function of SP cells discharging chemotherapeutic agents.