[Objective] North Cordyceps is as valuable as the wild Cordyceps nensis on medicinal value, nutrients, and trace element contents, which attracts lots of researchers to explore the cultivation method. In order to mas...[Objective] North Cordyceps is as valuable as the wild Cordyceps nensis on medicinal value, nutrients, and trace element contents, which attracts lots of researchers to explore the cultivation method. In order to master the best way to increase output, an experiment was conducted [Methods] To conclude the result, 100 boxes were chosen, of which 50 boxes were treated with fungiscratching in the same condition, based on comparisons of color changing and fruit body. [Results] The stroma have consistent length in good order ,but there is 1 box of scratching happened infections after operating. [Conclusion] Scratching can promote mycelium turning color better, shorter the cycle of fruiting, and prevent the phenomenon of ＂aside grass＂; and ＂no grass＂; effectively,al of this can make the North Cordyceps grow more dense and neatly.展开更多
Synergism between pesticide and fertilizer in controlling root-knot nematode was investigated by field randomized block test. The results showed that the best control efficiency was shared by 0.5% abamectin and 25% mi...Synergism between pesticide and fertilizer in controlling root-knot nematode was investigated by field randomized block test. The results showed that the best control efficiency was shared by 0.5% abamectin and 25% mixed pesticide （abamectin and carbosulfan）, 24.1% and 28.0%, respectively. The positive effect was found by synergism between pesticide and fertiUzer in controlling reot-knot nematode. The economic benefit and agricultural benefit were gained by controlling root-knot nematodes. The results suggested that the agricultural and ecological benefit could be gained through the integration of agricultural practice and chemical technology in controlling root-knot nematode.展开更多
AIM: To investigate the effects of Danshaohuaxian (DSHX), a Chinese herbal recipe, on the apoptosis and cell cycles of hepatic stellate cells (HSCs) in rat hepatic fibrosis and its possible mechanisms. METHODS: Sevent...AIM: To investigate the effects of Danshaohuaxian (DSHX), a Chinese herbal recipe, on the apoptosis and cell cycles of hepatic stellate cells (HSCs) in rat hepatic fibrosis and its possible mechanisms. METHODS: Seventy-six male Wistar rats were randomly divided into normal control group, hepatic fibrosis group, non-DSHX-treated group and DSHX-treated group. Except for the normal control group, rat hepatic fibrotic models were induced by subcutaneous injection of carbon tetrachloride (CCl4), drinking alcohol, giving diet of hyperlipid and hypoprotein for 8 wk. When the hepatic fibrotic models were produced, 12 rats of hepatic fibrosis group (15 rats survived, others died during the 8 wk) were sacrificed to collect blood and livers. HSCs were isolated from the other 3 rats to detect the apoptotic index (AI) and cell cycles by flow cytometry. DSHX was then given to the DSHX-treated group (1.0 g/kg, PO, daily) for 8 wk. At the same time, normal control group and non-DSHX-treated group were given normal saline for 8 wk. At end of the experiment, some rats in these three groups were sacrificed to collect blood and livers, the other rats were used for HSC isolation to detect the apoptotic index (AI) and cell cycles. Then the liver index, serum hyaluronic acid (HA) and alanine aminotransferase (ALT), degree of hepatic fibrosis, urinary excretion of hydroxyproline (Hyp) and expression of collagen types Ⅰ and Ⅲ (COL Ⅰ and Ⅲ) in these four groups were detected respectively. RESULTS: Compared with the indexes of the hepatic fibrosis group and non-DSHX-treated group, the DSHX-treated group revealed a liver index of (0.0267±0.0017 vs 0.0423±0.0044, 0.0295±0.0019, P<0.05), levels of serum HA (200.78±31.71 vs 316.17±78.48, 300.86±72.73, P<0.05) and ALT(93.13±5.79 vs 174.5±6.02, 104.75±6.54, P<0.01), and stage of hepatic fibrosis (1.30 vs 4.25, 2.60, P<0.01) all reduced. The urinary excretion of Hyp increased (541.09±73.39 vs 62.00±6.40, 182.44±30.83, P<0.01), the COL Ⅰ and Ⅲ expression decreased (COL I: 1.07±0.96 vs 4.18±2.26, 3.22±1.44, P<0.01; COL Ⅲ: 1.09±0.58 vs 3.04±0.62, 2.23±0.58, P<0.01), the HSCs apoptotic index of HSCs (7.81±0.47 vs 1.63±0.25, 1.78±0.4, P<0.05) and the ratio of G0-G1 phase cells increased (94.30±1.33 vs 62.27±17.96, 50.53±2.25, P<0.05). The ratios of S-phase cells (3.11±1.27 vs 9.83±1.81, 11.87±1.9, P<0.05) and G2-M phase cells (2.58±0.73 vs 23.26±10.95, 13.60±1.15, P<0.01) declined. CONCLUSION: DSHX capsule shows certain therapeutic effects on hepatic fibrosis in rats and inhibits abnormal deposition of COL I and III in rat livers by promoting the apoptosis of HSCs and preventing their proliferation.展开更多
To approach the mechanism of lipopolysaccharide (LPS) in causing acute lung injury (ALI) and the protective effect of rhubarb and dexamethasone, lung specimens were examined with macroscopy, microscopy, electron micro...To approach the mechanism of lipopolysaccharide (LPS) in causing acute lung injury (ALI) and the protective effect of rhubarb and dexamethasone, lung specimens were examined with macroscopy, microscopy, electron microscopy and the biological markers of ALI including lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary capillary permeability and pulmonary alveolar permeability index were observed. The mechanism of the ALI is mainly due to direct injury of alveolar epithelium and pulmonary vascular endothelium. Rhubarb and dexamethasone could significantly reduce the edema of the lung tissue, decrease the red blood cell exudation, neutrophil infiltration and plasma protein exudation in the alveoli and all the biological markers in comparison with the ALI model rats, indicating they have protective action on vascular endothelium and alveolar epithelium.展开更多
Jamu is traditional herbal medicine and a healthy drink considered by some Indonesians to be a primary element in their lives. The term ofjamu may be derived from ancient Javanese language ＂Djampi＂ that means a heal...Jamu is traditional herbal medicine and a healthy drink considered by some Indonesians to be a primary element in their lives. The term ofjamu may be derived from ancient Javanese language ＂Djampi＂ that means a healing method using herb during the era of Ancient Java emerged since the 9th century AD. Jamu is consume either in fresh or in processed one. Some jamu sellers still found carrying their wares, in a basket fastened to their torsos using a sling made of fabric, and visit door to door in local neighborhoods. The name is Jamu gendong. The use of the j amu remains the same as the ancestors did. The visible proof is the use of traditional herbal medicine of various type of ＂medical plants＂, either from the leaves, the fruits, the roots, the flowers or the barks, etc. It is truly back to nature. These herbal medicine had been used since the ancient time up to now, it is largely consumed by people of different levels： lower, middle and upper, in the villages and in the big cities. Jamu as a cultural heritage has a big potential asset of Indonesia and is also one of Indonsia＇s cultural products based on local resources and the creativity of the nation. Indonesia has natural resourses of plants totaling about 30,000 species of flowering plants, which is the third largest in the world after Brazil and Zaire, including 7,000 species of medicinal plants, 940 species one could buy easily readymade jamu packed modernly in the form of powder, pills, capsules, drinking liquid and ointments. Of course there are still jamu shops, which only sell for those have been identified, 283 species are listed as raw materials that are used routinely in the medicine industry. Jamu from time to time has been widely accepted in almost every country in the world. It is not only as a preventive maintenance, but also as a curative treatment for acute and chronic treatment. At present ingredients ofjamu and its preparation spot as required by buyers. Some women are roaming the street to sell jamu, which is a common view across the country. At present time, jamu has also been produced in mass by manufacturers for export, and mostly concerns on quality, consistency, and cleanliness either locally or internationally distributed.展开更多
AIM: Taraxacum officinale (TO) has been frequently used as a remedy for inflammatory diseases. The aim of this study was to investigate the effect of TO on cholecystokinin (CCK)-octapeptide-induced acute pancreatitis ...AIM: Taraxacum officinale (TO) has been frequently used as a remedy for inflammatory diseases. The aim of this study was to investigate the effect of TO on cholecystokinin (CCK)-octapeptide-induced acute pancreatitis in rats.METHODS: TO at 10 mg/kg was orally administered, followed by 75 μg/kg CCK octapeptide injected subcutaneously three times after 1, 3 and 5 h. This whole procedure was repeated for 5 d. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60 and HSP72, and the secretion of pro-inflammatory cytokines. Repeated CCK octapeptide treatment resulted in typical laboratory and morphological changes of experimentally-induced pancreatitis.RESULTS: TO significantly decreased the pancreatic weight/body weight ratio in CCK octapeptide-induced acute pancreatitis. TO also increased the pancreatic levels of HSP60 and HSP72. Additionally, the secretion of IL-6 and TNF-α decreased in the animals treated with TO.CONCLUSION: TO may have a protective effect against CCK octapeptide-induced acute pancreatitis.展开更多
文摘[Objective] North Cordyceps is as valuable as the wild Cordyceps nensis on medicinal value, nutrients, and trace element contents, which attracts lots of researchers to explore the cultivation method. In order to master the best way to increase output, an experiment was conducted [Methods] To conclude the result, 100 boxes were chosen, of which 50 boxes were treated with fungiscratching in the same condition, based on comparisons of color changing and fruit body. [Results] The stroma have consistent length in good order ,but there is 1 box of scratching happened infections after operating. [Conclusion] Scratching can promote mycelium turning color better, shorter the cycle of fruiting, and prevent the phenomenon of ＂aside grass＂; and ＂no grass＂; effectively,al of this can make the North Cordyceps grow more dense and neatly.
基金Supported by Yunnan Tobacco Company Program(2015YN192014YN25)~~
文摘Synergism between pesticide and fertilizer in controlling root-knot nematode was investigated by field randomized block test. The results showed that the best control efficiency was shared by 0.5% abamectin and 25% mixed pesticide （abamectin and carbosulfan）, 24.1% and 28.0%, respectively. The positive effect was found by synergism between pesticide and fertiUzer in controlling reot-knot nematode. The economic benefit and agricultural benefit were gained by controlling root-knot nematodes. The results suggested that the agricultural and ecological benefit could be gained through the integration of agricultural practice and chemical technology in controlling root-knot nematode.
文摘AIM: To investigate the effects of Danshaohuaxian (DSHX), a Chinese herbal recipe, on the apoptosis and cell cycles of hepatic stellate cells (HSCs) in rat hepatic fibrosis and its possible mechanisms. METHODS: Seventy-six male Wistar rats were randomly divided into normal control group, hepatic fibrosis group, non-DSHX-treated group and DSHX-treated group. Except for the normal control group, rat hepatic fibrotic models were induced by subcutaneous injection of carbon tetrachloride (CCl4), drinking alcohol, giving diet of hyperlipid and hypoprotein for 8 wk. When the hepatic fibrotic models were produced, 12 rats of hepatic fibrosis group (15 rats survived, others died during the 8 wk) were sacrificed to collect blood and livers. HSCs were isolated from the other 3 rats to detect the apoptotic index (AI) and cell cycles by flow cytometry. DSHX was then given to the DSHX-treated group (1.0 g/kg, PO, daily) for 8 wk. At the same time, normal control group and non-DSHX-treated group were given normal saline for 8 wk. At end of the experiment, some rats in these three groups were sacrificed to collect blood and livers, the other rats were used for HSC isolation to detect the apoptotic index (AI) and cell cycles. Then the liver index, serum hyaluronic acid (HA) and alanine aminotransferase (ALT), degree of hepatic fibrosis, urinary excretion of hydroxyproline (Hyp) and expression of collagen types Ⅰ and Ⅲ (COL Ⅰ and Ⅲ) in these four groups were detected respectively. RESULTS: Compared with the indexes of the hepatic fibrosis group and non-DSHX-treated group, the DSHX-treated group revealed a liver index of (0.0267±0.0017 vs 0.0423±0.0044, 0.0295±0.0019, P<0.05), levels of serum HA (200.78±31.71 vs 316.17±78.48, 300.86±72.73, P<0.05) and ALT(93.13±5.79 vs 174.5±6.02, 104.75±6.54, P<0.01), and stage of hepatic fibrosis (1.30 vs 4.25, 2.60, P<0.01) all reduced. The urinary excretion of Hyp increased (541.09±73.39 vs 62.00±6.40, 182.44±30.83, P<0.01), the COL Ⅰ and Ⅲ expression decreased (COL I: 1.07±0.96 vs 4.18±2.26, 3.22±1.44, P<0.01; COL Ⅲ: 1.09±0.58 vs 3.04±0.62, 2.23±0.58, P<0.01), the HSCs apoptotic index of HSCs (7.81±0.47 vs 1.63±0.25, 1.78±0.4, P<0.05) and the ratio of G0-G1 phase cells increased (94.30±1.33 vs 62.27±17.96, 50.53±2.25, P<0.05). The ratios of S-phase cells (3.11±1.27 vs 9.83±1.81, 11.87±1.9, P<0.05) and G2-M phase cells (2.58±0.73 vs 23.26±10.95, 13.60±1.15, P<0.01) declined. CONCLUSION: DSHX capsule shows certain therapeutic effects on hepatic fibrosis in rats and inhibits abnormal deposition of COL I and III in rat livers by promoting the apoptosis of HSCs and preventing their proliferation.
文摘To approach the mechanism of lipopolysaccharide (LPS) in causing acute lung injury (ALI) and the protective effect of rhubarb and dexamethasone, lung specimens were examined with macroscopy, microscopy, electron microscopy and the biological markers of ALI including lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary capillary permeability and pulmonary alveolar permeability index were observed. The mechanism of the ALI is mainly due to direct injury of alveolar epithelium and pulmonary vascular endothelium. Rhubarb and dexamethasone could significantly reduce the edema of the lung tissue, decrease the red blood cell exudation, neutrophil infiltration and plasma protein exudation in the alveoli and all the biological markers in comparison with the ALI model rats, indicating they have protective action on vascular endothelium and alveolar epithelium.
文摘Jamu is traditional herbal medicine and a healthy drink considered by some Indonesians to be a primary element in their lives. The term ofjamu may be derived from ancient Javanese language ＂Djampi＂ that means a healing method using herb during the era of Ancient Java emerged since the 9th century AD. Jamu is consume either in fresh or in processed one. Some jamu sellers still found carrying their wares, in a basket fastened to their torsos using a sling made of fabric, and visit door to door in local neighborhoods. The name is Jamu gendong. The use of the j amu remains the same as the ancestors did. The visible proof is the use of traditional herbal medicine of various type of ＂medical plants＂, either from the leaves, the fruits, the roots, the flowers or the barks, etc. It is truly back to nature. These herbal medicine had been used since the ancient time up to now, it is largely consumed by people of different levels： lower, middle and upper, in the villages and in the big cities. Jamu as a cultural heritage has a big potential asset of Indonesia and is also one of Indonsia＇s cultural products based on local resources and the creativity of the nation. Indonesia has natural resourses of plants totaling about 30,000 species of flowering plants, which is the third largest in the world after Brazil and Zaire, including 7,000 species of medicinal plants, 940 species one could buy easily readymade jamu packed modernly in the form of powder, pills, capsules, drinking liquid and ointments. Of course there are still jamu shops, which only sell for those have been identified, 283 species are listed as raw materials that are used routinely in the medicine industry. Jamu from time to time has been widely accepted in almost every country in the world. It is not only as a preventive maintenance, but also as a curative treatment for acute and chronic treatment. At present ingredients ofjamu and its preparation spot as required by buyers. Some women are roaming the street to sell jamu, which is a common view across the country. At present time, jamu has also been produced in mass by manufacturers for export, and mostly concerns on quality, consistency, and cleanliness either locally or internationally distributed.
文摘AIM: Taraxacum officinale (TO) has been frequently used as a remedy for inflammatory diseases. The aim of this study was to investigate the effect of TO on cholecystokinin (CCK)-octapeptide-induced acute pancreatitis in rats.METHODS: TO at 10 mg/kg was orally administered, followed by 75 μg/kg CCK octapeptide injected subcutaneously three times after 1, 3 and 5 h. This whole procedure was repeated for 5 d. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60 and HSP72, and the secretion of pro-inflammatory cytokines. Repeated CCK octapeptide treatment resulted in typical laboratory and morphological changes of experimentally-induced pancreatitis.RESULTS: TO significantly decreased the pancreatic weight/body weight ratio in CCK octapeptide-induced acute pancreatitis. TO also increased the pancreatic levels of HSP60 and HSP72. Additionally, the secretion of IL-6 and TNF-α decreased in the animals treated with TO.CONCLUSION: TO may have a protective effect against CCK octapeptide-induced acute pancreatitis.
