发根土壤杆菌对葛属药用植物的遗传转化

葛根是传统中药。利用发根土壤杆菌 (Agrobacteriumrhizogenes (Rikeretal.)Conn)R16 0 1转化野葛 (Puerarialobata (Willd .)Ohwi)、山葛 (P .lobatavar.montana)和三裂叶野葛 (P .phaseoloides (Roxb .)Benth .)离体叶片 ,在叶片表面直接形成毛状根。毛状根的诱导频率分别为 16 .6 %、16 .2 %和 2 6 .6 %。三种药用葛毛状根均表现出生长快、多分支、负向重力性的典型特征。毛状根离体培养均具有激素自主特性和抗卡那霉素特性 ,毛状根的冠瘿碱纸电泳、rol基因扩增及分子杂交实验均证实了其转基因性质。

Treatment of 65 Cases of Cervical Vertigo Treated by Acupuncture plus Medication

Objective： To investigate the efficacy of acupuncture plus puerarin and glucose injection for treating cervical vertigo. Method： 110 cases of cervical vertigo were divided into the treatment group and control group. 65 cases in the treatment group were treated by acupuncture plus puerarin and glucose injection and 45 cases in the control group were treated with Western medications. Results： In the treatment group, the results showed cure in 33 cases, effect in 29 cases and failure in 3 cases, and the total effective rate in 95.38%. In the control group, the results showed cure in 12 cases, effect in 23 cases and failure in 10 cases, and the total effective rate in 77.87%. In comparison of the total effective rate between the two groups, the treatment group was better than the control group （P〈0.01）. Conclusion： The therapeutic effect is better in the treatment of cervical vertigo by acupuncture plus herbal medicine.than that by Western medications.