The concept of“pharmacophylogeny”was proposed by Peigen Xiao in the 1980s based on long-term studies of Chinese researchers since ancient times and especially the 1950s.The complicated relationships and connectivity...The concept of“pharmacophylogeny”was proposed by Peigen Xiao in the 1980s based on long-term studies of Chinese researchers since ancient times and especially the 1950s.The complicated relationships and connectivity between kinship of medicinal plants,their chemical profiles and therapeutic utilities are consistent goals of pharmacophylogeny studies,which benefit innovative drug R&D.In the present work,we reviewed the origin and a brief history of research in this field,as well as the status quo and recent progress of pharmacophylogeny.The concept“pharmacophylogenomics”is put forward to represent the expanding utility of pharmacophylogeny in botanical drug R&D.Pharmacophylogeny and pharmacophylogenomics are the synthesis of multiple disciplines,such as chemotaxonomy,plant morphology,plant biochemistry/molecular biology and omics,etc.Medicinal plants within the same phylogenetic groups may have the same or similar therapeutic compounds/effects,thus forming the core of pharmacophylogeny,which is the scientific law summed up from practice and applied to practice after refining and sublimation.In the past,pharmacophylogeny plays a big role in looking for alternative resources of imported drugs in China.At present,it continues to play an active role in expanding medicinal plant resources,quality control/identification of herbal medicines,as well as predicting the chemical constituents or active ingredients of herbal medicine and the identification and determination of chemical constituents.In the ongoing future,it will play a bigger role in the search for new drugs,sorting out,summarizing,and improving herbal medicine experiences,thus boosting the sustainable conservation and utilization of traditional/natural medicinal resources.展开更多
文摘目的：分析不同季节杨梅叶挥发油（Myrica rubra leaves volatile oils,MVO）的化学成分变化规律,探讨其抗癌抑菌等生物活性及药用价值。方法：通过水蒸气蒸馏法提取挥发油,利用GC-MS技术表征挥发油的季节差异性,并通过MTT法分析对人肺癌细胞A549增殖抑制作用,琼脂扩散法分析对6种细菌的抑菌活性。结果：春、夏、秋和冬四季杨梅叶挥发油提取得率为每100 g 0.09∽0.13 m L,分别鉴定出39、30、28和41种成分,其中有16种萜烯、醇、醛、酮、酸和酯类物质存在于所有的叶片中。β-石竹烯和α-葎草烯是杨梅叶挥发油的主要成分,β-石竹烯含量的季节变化规律为冬季（43%）〉春季（28.9%）〉秋季（22.9%）〉夏季（19.02%）,α-葎草烯含量的季节变化规律为秋季（39.8%）〉春季（31.5%）〉冬季（26.02%）〉夏季（25.1%）。另外挥发油中含量超过15%的成分有秋季挥发油中的γ-雪松烯和夏季挥发油中的喇叭烯氧化物-（Ⅱ）,此2种成分在不同的季节中含量变化差异显著,氧化石竹烯含量在春冬两季含量大于4%,秋季未检出,除此以外其他成分含量均较低。MTT实验结果表明,杨梅叶挥发油对细胞A549增殖的抑制随作用时间增加而增强,且冬季挥发油对肿瘤细胞具有较强的细胞毒活性。抑菌实验表明杨梅叶挥发油对革兰氏阳性菌的抑制作用强于对革兰氏阴性菌,而冬季挥发油抑菌效果强于其他3个季节,对变形杆菌和沙门氏菌的抑制能力较弱。结论：杨梅叶挥发油具有抑菌、抗癌等药物开发前景,且在冬季杨梅叶挥发油作用最为显著。
基金National Natural Science Foundation of China(Grant No.41977048)Natural Science Fund of Liaoning Province(Grant No.20180550190)the Scientific Research Funds Project of Liaoning Education Department(Grant No.JDL2019012)。
文摘The concept of“pharmacophylogeny”was proposed by Peigen Xiao in the 1980s based on long-term studies of Chinese researchers since ancient times and especially the 1950s.The complicated relationships and connectivity between kinship of medicinal plants,their chemical profiles and therapeutic utilities are consistent goals of pharmacophylogeny studies,which benefit innovative drug R&D.In the present work,we reviewed the origin and a brief history of research in this field,as well as the status quo and recent progress of pharmacophylogeny.The concept“pharmacophylogenomics”is put forward to represent the expanding utility of pharmacophylogeny in botanical drug R&D.Pharmacophylogeny and pharmacophylogenomics are the synthesis of multiple disciplines,such as chemotaxonomy,plant morphology,plant biochemistry/molecular biology and omics,etc.Medicinal plants within the same phylogenetic groups may have the same or similar therapeutic compounds/effects,thus forming the core of pharmacophylogeny,which is the scientific law summed up from practice and applied to practice after refining and sublimation.In the past,pharmacophylogeny plays a big role in looking for alternative resources of imported drugs in China.At present,it continues to play an active role in expanding medicinal plant resources,quality control/identification of herbal medicines,as well as predicting the chemical constituents or active ingredients of herbal medicine and the identification and determination of chemical constituents.In the ongoing future,it will play a bigger role in the search for new drugs,sorting out,summarizing,and improving herbal medicine experiences,thus boosting the sustainable conservation and utilization of traditional/natural medicinal resources.
