以黄山毛峰为原料,采用实时荧光定量反转录聚合酶链式反应法和蛋白免疫印迹法研究其茶多酚提取物对肝药酶Cyp3a11及孕烷X受体(pregnane X receptor,PXR)的mRNA和蛋白表达影响,在此基础上采用瞬时共转染报告基因技术进一步验证是否通过PX...以黄山毛峰为原料,采用实时荧光定量反转录聚合酶链式反应法和蛋白免疫印迹法研究其茶多酚提取物对肝药酶Cyp3a11及孕烷X受体(pregnane X receptor,PXR)的mRNA和蛋白表达影响,在此基础上采用瞬时共转染报告基因技术进一步验证是否通过PXR实现对Cyp3a11表达调控。结果表明:黄山毛峰茶多酚提取物各剂量组(75、150、300 mg/(kg·d))均能显著调控小鼠肝Cyp3a11、PXR m RNA及其蛋白表达;报告基因实验结果显示,100、200、300μg/m L黄山毛峰茶多酚提取物经PXR通路使细胞色素P4503A4基因荧光素酶活力分别增加(3.86±0.05)、(4.82±0.72)、(5.38±0.11)倍。研究表明黄山毛峰茶多酚提取物可激活PXR通路调控肝药酶Cyp3a11的表达。展开更多
Objective To observe the scopolamine on the lull ation of pentobarbital sodium of mice. Methods Took three groups in mice, then the group of chloramphenicol (25mg·kg^-1), the group of scopolamine (0.06mg·kg^...Objective To observe the scopolamine on the lull ation of pentobarbital sodium of mice. Methods Took three groups in mice, then the group of chloramphenicol (25mg·kg^-1), the group of scopolamine (0.06mg·kg^-1), and the group of 0.9 % sodium chloride, injected the equal capacity of drug for leave each other. After 30 minutes, the injection of pentobarbital sodium (40mg·kg^-1) was performed on mice, to observe the time of lull preserve on mice. Results The group injected with chloramphenicol (25mg·kg^-1) have extended the time of the lull preserve of mice compared with 0.9 % sodium chloride, it has an absolute distance. The group of injected with scopolamine (0.06mg·kg^-1) have strong ation of increased the time of lull preserve on mice compared with 0.9 % sodium chloride. It has absolute distance. Conclusions The scopolamine has inhibitory effect on the activity of hepatic microsomal enzyme, it has increase the time of lull preserve.展开更多
文摘以黄山毛峰为原料,采用实时荧光定量反转录聚合酶链式反应法和蛋白免疫印迹法研究其茶多酚提取物对肝药酶Cyp3a11及孕烷X受体(pregnane X receptor,PXR)的mRNA和蛋白表达影响,在此基础上采用瞬时共转染报告基因技术进一步验证是否通过PXR实现对Cyp3a11表达调控。结果表明:黄山毛峰茶多酚提取物各剂量组(75、150、300 mg/(kg·d))均能显著调控小鼠肝Cyp3a11、PXR m RNA及其蛋白表达;报告基因实验结果显示,100、200、300μg/m L黄山毛峰茶多酚提取物经PXR通路使细胞色素P4503A4基因荧光素酶活力分别增加(3.86±0.05)、(4.82±0.72)、(5.38±0.11)倍。研究表明黄山毛峰茶多酚提取物可激活PXR通路调控肝药酶Cyp3a11的表达。
文摘Objective To observe the scopolamine on the lull ation of pentobarbital sodium of mice. Methods Took three groups in mice, then the group of chloramphenicol (25mg·kg^-1), the group of scopolamine (0.06mg·kg^-1), and the group of 0.9 % sodium chloride, injected the equal capacity of drug for leave each other. After 30 minutes, the injection of pentobarbital sodium (40mg·kg^-1) was performed on mice, to observe the time of lull preserve on mice. Results The group injected with chloramphenicol (25mg·kg^-1) have extended the time of the lull preserve of mice compared with 0.9 % sodium chloride, it has an absolute distance. The group of injected with scopolamine (0.06mg·kg^-1) have strong ation of increased the time of lull preserve on mice compared with 0.9 % sodium chloride. It has absolute distance. Conclusions The scopolamine has inhibitory effect on the activity of hepatic microsomal enzyme, it has increase the time of lull preserve.
