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油莎草提取物对慢性应激诱导小鼠抑郁样行为的改善和作用机制初探 被引量:4
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作者 敬思群 陈菲 +2 位作者 叶俊 刘根梅 张俊艳 《中国食物与营养》 2022年第8期70-76,共7页
目的:研究油莎草提取物对慢性温和不可预见性应激(CUMS)小鼠的抗抑郁功效及机制。方法:采用CUMS程序持续6 w建立抑郁模型,造模成功后,随机将小鼠分为模型组、阳性组、油莎草须根水提物剂量组、油莎草须根醇提物剂量组、油莎草地上茎叶... 目的:研究油莎草提取物对慢性温和不可预见性应激(CUMS)小鼠的抗抑郁功效及机制。方法:采用CUMS程序持续6 w建立抑郁模型,造模成功后,随机将小鼠分为模型组、阳性组、油莎草须根水提物剂量组、油莎草须根醇提物剂量组、油莎草地上茎叶水提物剂量组、油莎草地上茎叶醇提物剂量组、油莎豆水提物剂量组、油莎豆醇提物剂量组,每组14只。灌胃给药,持续30 d,模型组和正常组灌胃等体积的双蒸水,阳性组氟西汀按20 mg/(kg·d)BW灌胃,其余6组皆按200 mg/(kg·d)BW灌胃。除正常对照组外,其余组均采用CUMS小鼠模型,并观察油莎草提取物对小鼠行为学、生化指标和皮质酮等指标的影响。每周进行体重称量,采用旷场实验、强迫游泳实验、糖水偏好实验等行为学方法评价小鼠的抑郁状态;采用试剂盒法测定小鼠血清MDA含量、SOD、GSH-PX活性等生化指标,采用酶联免疫分析(ELISA)法测定小鼠血清皮质酮含量。结果:与正常组比较,模型组小鼠生长缓慢,运动功能明显受到抑制,小鼠水平、垂直运动得分降低(P<0.01),游泳不动时间明显延长,糖水相对消耗量明显降低(P<0.01);血清SOD、GSH-Px活性降低,MDA含量明显增高(P<0.01),皮质酮水平明显升高(P<0.01);与模型组比较,油莎草提取物各组小鼠生长正常,小鼠水平、垂直运动得分提高(P<0.01),游泳不动时间显著缩短(P<0.01),蔗糖水相对消耗量明显升高(P<0.01);血清SOD、GSH-Px活力提高,MDA含量明显降低(P<0.01),小鼠血清皮质酮水平明显降低(P<0.01);与正常组比较无统计学差异。结论:油莎草提取物对慢性应激所引起的抑郁症状有一定的改善功效,其作用机制可能与抑制HPA轴功能紊乱,改善机体自由基损伤状态,增强SOD、GSH-Px活力,降低MDA含量等多种途径有关。 展开更多
关键词 莎草提取 抗抑郁作用 行为学 生化指标 皮质酮水平
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关于中国东北产薹草属(Carex L.)六种植物的修订
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作者 杨雅玲 《南京大学学报(自然科学版)》 CAS CSCD 1991年第4期804-808,共5页
本文对曾经已发表过的一些东北产莎草科薹草属植物(Carex mongolica Baranov et Skvortzov,C.cobresiaeformis Baranov et Skvortzov,C.chelungkiangnica Baranov et Skvortzov,C.yingkiliensis Baranov et Skvortzov,C.komaroviana Bar... 本文对曾经已发表过的一些东北产莎草科薹草属植物(Carex mongolica Baranov et Skvortzov,C.cobresiaeformis Baranov et Skvortzov,C.chelungkiangnica Baranov et Skvortzov,C.yingkiliensis Baranov et Skvortzov,C.komaroviana Baranov et Skvortzov)加以讨论修订,这些植物不应是新种,它们应该分别属于过去已发表过的种类:Carex lithopila Turcz.,C.gynocrates Wormskj.ex Dreier.,C.cinerascens Kaenth.,C.mollissima Christ ex Scheutz.C.angarae steud。 展开更多
关键词 薹草属 莎草物 修订
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油莎草醇提物成分分离鉴定及抗氧化活性分析 被引量:7
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作者 王赛赛 张孟凡 +3 位作者 齐蕊 敬思群 涂亦娴 王为兰 《食品工业》 北大核心 2017年第6期185-189,共5页
以油莎草醇提物为原料,利用D101大孔树脂,依次用0,30%,50%,70%和95%乙醇对油莎草醇提物进行梯度洗脱,以DPPH·自由基清除率为考察指标,确定抗氧化活性最强的洗脱部位(目标醇提物),薄层层析(TLC)定性分析,利用50目ODS RP-18反相硅胶... 以油莎草醇提物为原料,利用D101大孔树脂,依次用0,30%,50%,70%和95%乙醇对油莎草醇提物进行梯度洗脱,以DPPH·自由基清除率为考察指标,确定抗氧化活性最强的洗脱部位(目标醇提物),薄层层析(TLC)定性分析,利用50目ODS RP-18反相硅胶对目标醇提物进行分离纯化,再经Sephadex LH-20葡聚糖凝胶柱分离得到近似单体化合物。利用核磁共振(NMR)对单体化合物进行结构鉴定。结果表明,30%乙醇洗脱部位的抗氧化活性最强,其IC50为0.050 68±0.092 74 mg·m L^(-1),弱于阳性对照VC(IC50为0.011 92±0.060 mg·m L^(-1))和芦丁(IC50为0.015 42±0.081 4 mg·m L^(-1)),从目标醇提物中分离得到4个单体化合物,化合物1为7-羟基-6-甲氧基-香豆素,化合物2、3为异荭草苷,化合物4为荭草苷。 展开更多
关键词 莎草醇提 抗氧化活性 分离纯化 结构鉴定
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油莎草醇提物对微循环和抗凝血活性作用初探 被引量:8
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作者 苏乐萍 王赛赛 敬思群 《食品工业》 北大核心 2017年第11期200-203,共4页
观察油莎草醇提物对小鼠耳廓微循环和抗凝血作用的影响。以低、中、高剂量的油莎草醇提物灌胃给药7 d后,用微循环显微镜观察小鼠耳廓血管口径,以剪尾法测定出血时间(BT),玻片法测定凝血时间(CT),应用体外测定凝血酶原时间(PT),观察不同... 观察油莎草醇提物对小鼠耳廓微循环和抗凝血作用的影响。以低、中、高剂量的油莎草醇提物灌胃给药7 d后,用微循环显微镜观察小鼠耳廓血管口径,以剪尾法测定出血时间(BT),玻片法测定凝血时间(CT),应用体外测定凝血酶原时间(PT),观察不同剂量的油莎草醇提物对耳廓微循环、出血时间、凝血时间及凝血酶原时间的影响。结果表明,油莎草醇提物能明显提高小鼠毛细血管口径,交叉节点数明显增加,提高微循环血流量,高剂量的油莎草醇提物交叉节点差值、血管口径差值分别为1.4±0.5,10.0±4.6,与空白对照组相比具有极显著差异(p<0.01)。油莎草醇提物随着剂量的增加明显延长小鼠出血时间,高剂量的油莎草醇提物组与空白对照组相比具有极显著差异(p<0.01),而油莎草醇提物也显著延长了凝血时间,其中高剂量组与阳性药物组的凝血时间相接近,而中剂量的油莎草醇提物极显著的延长了小鼠凝血酶原时间的出血时间(p<0.01)。油莎草醇提物具有促进小鼠血液微循环和提高抗凝血作用。 展开更多
关键词 莎草醇提 微循环 抗凝血
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Biological Characteristies of Methane Emission of Oryzasativa,Panicumcrus-galliand Cyperusdif for misGrown on PaddySoil 被引量:2
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作者 MINHANG CHENMEI-CI 《Pedosphere》 SCIE CAS CSCD 1994年第2期165-172,共8页
Biological characteristics of methane emission were compared among Oryza sativa, Panicum crus-galli andCyperus difformis grown on paddy soil. The order of the amounts of methane emitted was P. crus-galli > O. sativ... Biological characteristics of methane emission were compared among Oryza sativa, Panicum crus-galli andCyperus difformis grown on paddy soil. The order of the amounts of methane emitted was P. crus-galli > O. sativa >C. difformis, with the former two 11 and 8 times as much as that of the latter, respectively. And it was just opposite tothat of the methane-forming activities of roots of the three plants. The diurnal changes of methane emission of thethree plants were relatively consistent with valleys during 11:00-14:00 and midnight and peaks during 8:00-11: 00,14:00- 20:00 and about 5:00 early morning. The methanogenic activites in rhizospheric soils were higher than those innonrhizospheric soils with P. crus-galli and O. sativa, but not with C. difformis. The pathways of methane release ofP. crus-galli and O . sativa were probably through the top gaps near the junctions of nodal plate and leaf sheath, how-ever in C. difformistopgaps from which methane could escape into atmosphere were not found. 展开更多
关键词 activity of methane formation Cyperus difformis methane emission Oryza sativa Panicum crus-galli
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Leaf and root phenology and biomass of Eriophorum vaginatum in response to warming in the Arctic 被引量:2
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作者 Ting Ma Thomas Parker +4 位作者 Ned Fetcher Steven L.Ungers Jon Gewirtzman Michael L.Moody Jianwu Tang 《Journal of Plant Ecology》 SCIE CSCD 2022年第5期1091-1105,共15页
The response of plant leaf and root phenology and biomass in the Arctic to global change remains unclear due to the lack of synchronous measurements of above-and belowground parts.Our objective was to determine the ph... The response of plant leaf and root phenology and biomass in the Arctic to global change remains unclear due to the lack of synchronous measurements of above-and belowground parts.Our objective was to determine the phenological dynamics of the above-and belowground parts of Eriophorum vaginatum in the Arctic and its response to warming.We established a common garden located at Toolik Lake Field Station;tussocks of E.vaginatum from three locations,Coldfoot,Toolik Lake and Sagwon,were transplanted into the common garden.Control and warming treatments for E.vaginatum were set up at the Toolik Lake during the growing seasons of 2016 and 2017.Digital cameras,a handheld sensor and minirhizotrons were used to simultaneously observe leaf greenness,normalized difference vegetation index and root length dynamics,respectively.Leaf and root growth rates of E.vaginatum were asynchronous such that the timing of maximal leaf growth(mid-july)was about 28 days earlier than that of root growth.Warming of air temperature by 1°C delayed the timing of leaf senescence and thus prolonged the growing season,but the temperature increase had no significant effect on root phenology.The seasonal dynamics of leaf biomass were affected by air temperature,whereas root biomass was correlated with soil thaw depth.Therefore,we suggest that leaf and root components should be considered comprehensively when using carbon and nutrient cycle models,as above-and belowground productivity and functional traits may have a different response to climate warming. 展开更多
关键词 Eriophorum vaginatum PHENOLOGY WARMING aboveground biomass belowground biomass
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